PMID- 30871098 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20201001 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 12 IP - 5 DP - 2019 Mar 12 TI - In Vitro Assessment of the Functional Dynamics of Titanium with Surface Coating of Hydroxyapatite Nanoparticles. LID - 10.3390/ma12050840 [doi] LID - 840 AB - Manipulation of implant surface characteristics constitutes a promising strategy for improving cell growth and tissue response on a variety of materials with different surface topographies. Mesenchymal progenitor cells with a capacity to respond to titanium surface stimuli and differentiate into osteoblasts were used to perform comparative tests between two different implant topographies, including their functional interaction with pre-osteoblasts directly seeded onto the implants. Functional analysis of nanostructured implant surfaces was performed by in vitro assay analysis. The machined surface of titanium implants (mach group) was used as a control and compared with a nanoparticle HA activated surface implant (nano group), developed by the deposition of pure crystalline hydroxyapatite. Cell culture on the nano group surface resulted in higher cell adhesion and cultured osteoblast viability compared with the mach group. Scanning electron microscope (SEM) images revealed a stable interaction, indicated by the presence of focal cell adhesion formation. These results together with positive mineralization assays showed the nano group to be an excellent scaffold for bone-implant integration. FAU - de Lima Cavalcanti, Jose Henrique AU - de Lima Cavalcanti JH AD - Department of Rehabilitation, Federal Fluminense University, Rio de Janeiro 20000-000, Brazil. josehenriquecavalcanti@yahoo.com.br. FAU - Matos, Patricia C AU - Matos PC AD - Oral Rehabilitation Carioca Centre, Campo Grande 79000-000, Brazil. p.robbs@hotmail.com. FAU - Depes de Gouvea, Cresus Vinicius AU - Depes de Gouvea CV AD - Department of Rehabilitation, Federal Fluminense University, Rio de Janeiro 20000-000, Brazil. cresusuff@gmail.com. FAU - Carvalho, Waldimir AU - Carvalho W AUID- ORCID: 0000-0002-2919-1125 AD - Department of Rehabilitation, Federal Fluminense University, Rio de Janeiro 20000-000, Brazil. homensdepalavra@gmail.com. FAU - Calvo-Guirado, Jose Luis AU - Calvo-Guirado JL AUID- ORCID: 0000-0002-4067-2253 AD - Department of Oral Surgery and Implantology, Universidad Catolica de Murcia, 30107 Murcia, Spain. jlcalvo@ucam.edu. FAU - Aragoneses, Juan Manuel AU - Aragoneses JM AD - Department of Dental Research, Universidad Federico Henriquez y Carvajal (UFHEC), Santo Domingo 10107, Dominican Republic. jaragoneses@ufhec.edu.do. FAU - Perez-Diaz, Leticia AU - Perez-Diaz L AD - Molecular Interactions Laboratory, Facultad de Ciencias, Universidad de la Republica, Igua 4225, Montevideo 11400, Uruguay. letperez@gmail.com. FAU - Gehrke, Sergio Alexandre AU - Gehrke SA AUID- ORCID: 0000-0002-5863-9101 AD - Department of Biotechnology, Biotecnos-Technology and Science, Cuareim 1483, Montevideo 11100, Uruguay. sergio.gehrke@hotmail.com. AD - Biotechnology Department, Catholic University of Murcia (UCAM), 30107 Murcia, Spain. sergio.gehrke@hotmail.com. LA - eng PT - Journal Article DEP - 20190312 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC6427539 OTO - NOTNLM OT - implants surface OT - mesenchymal cells OT - titanium implants COIS- The authors declare no conflict of interest. EDAT- 2019/03/16 06:00 MHDA- 2019/03/16 06:01 PMCR- 2019/03/12 CRDT- 2019/03/16 06:00 PHST- 2019/01/16 00:00 [received] PHST- 2019/03/07 00:00 [revised] PHST- 2019/03/08 00:00 [accepted] PHST- 2019/03/16 06:00 [entrez] PHST- 2019/03/16 06:00 [pubmed] PHST- 2019/03/16 06:01 [medline] PHST- 2019/03/12 00:00 [pmc-release] AID - ma12050840 [pii] AID - materials-12-00840 [pii] AID - 10.3390/ma12050840 [doi] PST - epublish SO - Materials (Basel). 2019 Mar 12;12(5):840. doi: 10.3390/ma12050840.