PMID- 30871432 OWN - NLM STAT- MEDLINE DCOM- 20210820 LR - 20210820 IS - 1476-8305 (Electronic) IS - 1028-415X (Linking) VI - 23 IP - 11 DP - 2020 Nov TI - Supplementation of dietary non-digestible oligosaccharides from birth onwards improve social and reduce anxiety-like behaviour in male BALB/c mice. PG - 896-910 LID - 10.1080/1028415X.2019.1576362 [doi] AB - Objective: The intestinal microbiota is acknowledged to be essential in brain development and behaviour. Their composition can be modulated by prebiotics such as short-chain galacto-oligosaccharides (scGOS) and long-chain fructo-oligosaccharide (lcFOS). Several studies reported potential health benefit of prebiotics on behaviour. As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice. Method: Healthy male BALB/cByJ mice received, from day of birth, a dietary supplement with or without 3% scGOS:lcFOS (9:1). Behavioural tests were performed pre-weaning, in adolescence, early adulthood and adulthood. We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels. Results: Compared to control fed mice, scGOS:lcFOS fed mice showed reduced anxiety-like and repetitive behaviour over time and improved social behaviour in adulthood. The serotonergic system in the prefrontal cortex (PFC) and somatosensory cortex (SSC) was affected by the scGOS:lcFOS. In the PFC, mRNA expression of brain-derived neurotrophic factor (Bdnf) was enhanced in scGOS:lcFOS fed mice. Although the bacterial diversity of the intestinal microbiota was unaffected by the scGOS:lcFOS diet, microbiota composition differed between the scGOS:lcFOS and the control fed mice over time. Moreover, an increased saccharolytic and decreased proteolytic fermentation activity were observed in caecum content. Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice. These data provide further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life and strengthen the insights in the interplay between the developing intestine and brain. FAU - Szklany, Kirsten AU - Szklany K AD - Division of Pharmacology, Utrecht Institute of Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, Netherlands. FAU - Wopereis, Harm AU - Wopereis H AUID- ORCID: 0000-0002-2103-1442 AD - Department of Gut Biology and Microbiology, Danone Nutricia Research, Utrecht, Netherlands. AD - Laboratory of Microbiology, Wageningen University & Research, Wageningen, Netherlands. FAU - de Waard, Cindy AU - de Waard C AD - Division of Pharmacology, Utrecht Institute of Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, Netherlands. FAU - van Wageningen, Thecla AU - van Wageningen T AD - Division of Pharmacology, Utrecht Institute of Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, Netherlands. FAU - An, Ran AU - An R AUID- ORCID: 0000-0002-5475-3353 AD - Laboratory of Microbiology, Wageningen University & Research, Wageningen, Netherlands. FAU - van Limpt, Kees AU - van Limpt K AD - Department of Gut Biology and Microbiology, Danone Nutricia Research, Utrecht, Netherlands. FAU - Knol, Jan AU - Knol J AD - Department of Gut Biology and Microbiology, Danone Nutricia Research, Utrecht, Netherlands. AD - Laboratory of Microbiology, Wageningen University & Research, Wageningen, Netherlands. FAU - Garssen, Johan AU - Garssen J AD - Division of Pharmacology, Utrecht Institute of Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, Netherlands. AD - Department of Immunology, Danone Nutricia Research, Utrecht, Netherlands. FAU - Knippels, Leon M J AU - Knippels LMJ AD - Division of Pharmacology, Utrecht Institute of Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, Netherlands. AD - Department of Immunology, Danone Nutricia Research, Utrecht, Netherlands. FAU - Belzer, Clara AU - Belzer C AUID- ORCID: 0000-0001-6922-836X AD - Laboratory of Microbiology, Wageningen University & Research, Wageningen, Netherlands. FAU - Kraneveld, Aletta D AU - Kraneveld AD AD - Division of Pharmacology, Utrecht Institute of Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, Netherlands. AD - Institute for Risk Assessment Sciences, Faculty of Veterinary Sciences, Utrecht University, Netherlands. LA - eng PT - Journal Article DEP - 20190314 PL - England TA - Nutr Neurosci JT - Nutritional neuroscience JID - 100892202 RN - 0 (Biogenic Monoamines) RN - 0 (Oligosaccharides) RN - 0 (Prebiotics) SB - IM MH - Animals MH - Anxiety/*microbiology/prevention & control MH - Behavior, Animal MH - Biogenic Monoamines/metabolism MH - Brain/metabolism MH - *Gastrointestinal Microbiome MH - Male MH - Mice, Inbred BALB C MH - Oligosaccharides/*administration & dosage MH - Prebiotics/*administration & dosage MH - *Social Behavior MH - Vocalization, Animal OTO - NOTNLM OT - Behaviour OT - SCFA OT - dietary supplementation from birth OT - early life OT - fructo-oligosaccharide OT - galacto-oligosaccharide OT - healthy mice OT - intestinal microbiota OT - prebiotics OT - serotonergic system EDAT- 2019/03/16 06:00 MHDA- 2021/08/21 06:00 CRDT- 2019/03/16 06:00 PHST- 2019/03/16 06:00 [pubmed] PHST- 2021/08/21 06:00 [medline] PHST- 2019/03/16 06:00 [entrez] AID - 10.1080/1028415X.2019.1576362 [doi] PST - ppublish SO - Nutr Neurosci. 2020 Nov;23(11):896-910. doi: 10.1080/1028415X.2019.1576362. Epub 2019 Mar 14.