PMID- 30872074
OWN - NLM
STAT- MEDLINE
DCOM- 20191105
LR  - 20221207
IS  - 1095-8274 (Electronic)
IS  - 1075-9964 (Linking)
VI  - 57
DP  - 2019 Jun
TI  - Wastewater biofilm formation on self-assembled monolayer surfaces using 
      elastomeric flow cells.
PG  - 11-18
LID - S1075-9964(19)30044-7 [pii]
LID - 10.1016/j.anaerobe.2019.03.005 [doi]
AB  - In anaerobic wastewater treatment, microbial biofilm is beneficial for efficient 
      substrate utilization and for preventing the wash-out of key microorganisms. By 
      providing solid supports, biofilm formation can be accelerated due to the early 
      initial adhesion of residing microbes. Alteration in surface properties is 
      therefore one such approach that helps us understand microbial interfacial 
      interaction. Here, self-assembled monolayers of alkanethiols with carboxyl 
      (-COOH), hydroxyl (-OH), and amine (-NH(2)) terminal moieties on gold (Au) 
      substrates were employed to study the initial adhesion of wastewater microbes. An 
      elastomeric flow cell was also utilized to simulate the environment of wastewater 
      bioreactor. Results from fluorescence in situ hybridization (FISH) portrayed more 
      enhanced microbial adhesion after 2 h on -NH(2) functional group with the 
      calculated surface coverage of 12.8 +/- 2.4% as compared to 7.7 +/- 1.6% on -COOH, 
      11.0 +/- 2.0% on -OH, and 1.2% on unmodified Au surfaces. This might be because of 
      concomitant electrostatic attraction between negatively-charged bacteria and 
      positively-charged (-NH(3)(+)) functional groups. Nevertheless, the average 
      surface coverage by individual biofilm clusters was 28.0 +/- 5.0 mum(2) and 
      32.0 +/- 9.0 mum(2) on -NH(2) and -OH surfaces, respectively, while -COOH surfaces 
      resulted in higher value (60.0 +/- 5.0 mum(2)) and no significant cluster formation 
      was observed on Au surfaces. Accordingly, the average inter-cluster distance 
      observed on -NH(2) surfaces was relatively smaller (3.0 +/- 0.6 mum) as compared to 
      that on other surfaces. Overall, these data suggest favorable initial biofilm 
      growth on more hydrophilic and positively-charged surfaces. Furthermore, the 
      analysis of the mean fluorescence intensity revealed preferred initial adhesion 
      of key microbes (archaea) on -OH and -NH(2) surfaces. Indeed, results obtained 
      from this study would be beneficial in designing selective biointerfaces for 
      certain biofilm carriers in a typical wastewater bioreactor. Importantly, our 
      elastomeric flow cell integrated with SAM-modified surfaces demonstrated an ideal 
      platform for high-throughput investigation of wastewater biofilm under controlled 
      environments.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Siddique, Arslan
AU  - Siddique A
AD  - Biotechnology Division, School of Bioresources and Technology, King Mongkut's 
      University of Technology Thonburi, Bang Khun Thian, Bangkok, 10150, Thailand.
FAU - Suraraksa, Benjaphon
AU  - Suraraksa B
AD  - Excellent Center for Waste Utilization and Management, National Center for 
      Genetic Engineering and Biotechnology, National Science and Technology 
      Development Agency, King Mongkut's University of Technology Thonburi, Bang Khun 
      Thian, Bangkok, 10150, Thailand.
FAU - Horprathum, Mati
AU  - Horprathum M
AD  - Optical Thin-Film Laboratory, National Electronics and Computer Technology 
      Center, National Science and Technology Development Agency, Pathumthani, 12120, 
      Thailand.
FAU - Oaew, Sukunya
AU  - Oaew S
AD  - Biochemical Engineering and Pilot Plant Research and Development Laboratory, 
      National Center for Genetic Engineering and Biotechnology, National Science and 
      Technology Development Agency, King Mongkut's University of Technology Thonburi, 
      Bang Khun Thian, Bangkok, 10150, Thailand.
FAU - Cheunkar, Sarawut
AU  - Cheunkar S
AD  - Biotechnology Division, School of Bioresources and Technology, King Mongkut's 
      University of Technology Thonburi, Bang Khun Thian, Bangkok, 10150, Thailand. 
      Electronic address: sarawut.che@mail.kmutt.ac.th.
LA  - eng
PT  - Journal Article
DEP - 20190312
PL  - England
TA  - Anaerobe
JT  - Anaerobe
JID - 9505216
RN  - 0 (Waste Water)
SB  - IM
MH  - Anaerobiosis
MH  - Biofilms/*growth & development
MH  - Bioreactors/*microbiology
MH  - Cell Adhesion
MH  - Surface Properties
MH  - Wastewater/*microbiology
MH  - Water Purification/*methods
OTO - NOTNLM
OT  - Biofilm
OT  - Biogas
OT  - PDMS flow cell
OT  - Self-assembled monolayers (SAMs)
OT  - Wastewater
EDAT- 2019/03/16 06:00
MHDA- 2019/11/07 06:00
CRDT- 2019/03/16 06:00
PHST- 2018/08/16 00:00 [received]
PHST- 2019/02/25 00:00 [revised]
PHST- 2019/03/05 00:00 [accepted]
PHST- 2019/03/16 06:00 [pubmed]
PHST- 2019/11/07 06:00 [medline]
PHST- 2019/03/16 06:00 [entrez]
AID - S1075-9964(19)30044-7 [pii]
AID - 10.1016/j.anaerobe.2019.03.005 [doi]
PST - ppublish
SO  - Anaerobe. 2019 Jun;57:11-18. doi: 10.1016/j.anaerobe.2019.03.005. Epub 2019 Mar 
      12.