PMID- 30873617
OWN - NLM
STAT- MEDLINE
DCOM- 20190729
LR  - 20190729
IS  - 1095-8649 (Electronic)
IS  - 0022-1112 (Linking)
VI  - 94
IP  - 5
DP  - 2019 May
TI  - Evolutionarily conserved vasa identifies embryonic and gonadal germ cells in 
      spinyhead croaker Collichthys lucidus.
PG  - 772-780
LID - 10.1111/jfb.13964 [doi]
AB  - In this study, a 2198 bp full-length cDNA of spinyhead croaker Collichthys 
      lucidus vasa gene encoding 616 amino-acid residues was obtained. Multiple 
      alignment revealed that C. lucidus vasa has eight conserved characteristic motifs 
      of the DEAD box protein family and has the highest identity to large yellow 
      croaker Larimichthys croceas. Reverse-transcription (RT)-PCR and Western blot 
      analyses indicated that the vasa messenger (m)RNA and Vasa protein are 
      specifically expressed in the gonads in both sexes. In situ hybridisation (ISH) 
      demonstrated that vasa RNA is exclusively detected in the germ cells in C. 
      lucidus gonads and its temporospatial expression reveals a dynamic pattern during 
      oogenesis. Surprisingly, C. lucidus vasa 3'UTR can direct stable and specific GFP 
      expression in the primordial germ cells (PGC) of medaka Oryzias latipes embryos. 
      Taken together, these results suggest that because C. lucidus vasa expression 
      delineates critical stages of oogenesis, it may be a useful molecular marker for 
      the identification of gonadal germ cells, facilitating the isolation and 
      utilization of germ cells in future study.
CI  - (c) 2019 The Fisheries Society of the British Isles.
FAU - Li, Yu
AU  - Li Y
AD  - Key Laboratory of East China Sea & Oceanic Fishery Resources Exploitation and 
      Utilization, Ministry of Agriculture, East China Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
AD  - Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, 
      Ministry of Education, Shanghai Ocean University, Shanghai, China.
AD  - International Research Center for Marine Biosciences, Ministry of Science and 
      Technology, Shanghai Ocean University, Shanghai, China.
AD  - National Demonstration Center for Experimental Fisheries Science Education 
      Shanghai Ocean University, Shanghai, China.
FAU - Song, Wei
AU  - Song W
AD  - Key Laboratory of East China Sea & Oceanic Fishery Resources Exploitation and 
      Utilization, Ministry of Agriculture, East China Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
FAU - Zhu, Yei Fei
AU  - Zhu YF
AD  - Key Laboratory of East China Sea & Oceanic Fishery Resources Exploitation and 
      Utilization, Ministry of Agriculture, East China Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
AD  - Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, 
      Ministry of Education, Shanghai Ocean University, Shanghai, China.
AD  - International Research Center for Marine Biosciences, Ministry of Science and 
      Technology, Shanghai Ocean University, Shanghai, China.
AD  - National Demonstration Center for Experimental Fisheries Science Education 
      Shanghai Ocean University, Shanghai, China.
FAU - Zhu, Tian Yu
AU  - Zhu TY
AD  - Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, 
      Ministry of Education, Shanghai Ocean University, Shanghai, China.
AD  - International Research Center for Marine Biosciences, Ministry of Science and 
      Technology, Shanghai Ocean University, Shanghai, China.
AD  - National Demonstration Center for Experimental Fisheries Science Education 
      Shanghai Ocean University, Shanghai, China.
FAU - Ma, Ling Bo
AU  - Ma LB
AD  - Key Laboratory of East China Sea & Oceanic Fishery Resources Exploitation and 
      Utilization, Ministry of Agriculture, East China Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
FAU - Li, Ming You
AU  - Li MY
AD  - Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, 
      Ministry of Education, Shanghai Ocean University, Shanghai, China.
AD  - International Research Center for Marine Biosciences, Ministry of Science and 
      Technology, Shanghai Ocean University, Shanghai, China.
AD  - National Demonstration Center for Experimental Fisheries Science Education 
      Shanghai Ocean University, Shanghai, China.
LA  - eng
GR  - This work was supported by the Basic Research Fund for State-Level Nonprofit 
      Research Institutes of ESCFRI, CAFS (#Dong2018Z01), the National Natural Science 
      Foundation of China (31,672,700, 31,372,520) and the "Dawn" Program of Shanghai 
      Education Commission (16SG42)./
PT  - Journal Article
DEP - 20190412
PL  - England
TA  - J Fish Biol
JT  - Journal of fish biology
JID - 0214055
RN  - 0 (DNA, Complementary)
RN  - 0 (Fish Proteins)
MH  - Animals
MH  - *Biological Evolution
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Female
MH  - Fish Proteins/chemistry/*genetics
MH  - Germ Cells/*cytology/metabolism
MH  - Gonads/metabolism
MH  - In Situ Hybridization
MH  - Male
MH  - Oogenesis
MH  - Oryzias/genetics
MH  - Perciformes/embryology/*genetics
MH  - Phylogeny
OTO - NOTNLM
OT  - Collichthys lucidus
OT  - vasa; visualisation
OT  - primordial germ cells
EDAT- 2019/03/16 06:00
MHDA- 2019/07/30 06:00
CRDT- 2019/03/16 06:00
PHST- 2018/10/23 00:00 [received]
PHST- 2019/03/13 00:00 [accepted]
PHST- 2019/03/16 06:00 [pubmed]
PHST- 2019/07/30 06:00 [medline]
PHST- 2019/03/16 06:00 [entrez]
AID - 10.1111/jfb.13964 [doi]
PST - ppublish
SO  - J Fish Biol. 2019 May;94(5):772-780. doi: 10.1111/jfb.13964. Epub 2019 Apr 12.