PMID- 30874618
OWN - NLM
STAT- MEDLINE
DCOM- 20201001
LR  - 20231006
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Mar 15
TI  - Pistacia vera L. oleoresin and levofloxacin is a synergistic combination against 
      resistant Helicobacter pylori strains.
PG  - 4646
LID - 10.1038/s41598-019-40991-y [doi]
LID - 4646
AB  - The increasing multidrug resistance in Helicobacter pylori, also correlated to 
      its biofilm-forming ability, underlines the need to search novel strategies to 
      improve the eradication rate. Natural compounds are proposed as 
      antibiotic-resistant-breakers capable to restore the efficacy of conventional 
      drugs. Aim of this work was to evaluate the capability of Pistacia vera L. 
      oleoresin (ORS) to synergize with levofloxacin (LVX) against resistant H. pylori 
      strains. The antimicrobial activity of P. vera L. ORS and LVX and their 
      combinations was determined by MIC/MBC (in neutral and acidic environments) and 
      checkerboard tests. The anti-biofilm effect was determined by biomass 
      quantification. In vivo Galleria mellonella model was used to confirm in vitro 
      data. Pistacia vera L. ORS and LVX MICs ranged respectively from 780 to 3120 mg/l 
      and from 0.12 to 2.00 mg/l, at pH 7.0 and 5.5. MBCs were similar to MICs. 
      Pistacia vera L. ORS was able to synergize with LVX, restoring its effectiveness 
      in LVX resistant strains. Pistacia vera L. ORS, LVX and their synergistic 
      combinations displayed significant biofilm reduction. Pistacia vera L. ORS and 
      LVX, showed protective effect against H. pylori infection on G. mellonella (62% 
      and 63% of survival, respectively). Pistacia vera L. ORS can be considered a 
      promising potentiator to restore the effectiveness of LVX tackling the H. pylori 
      antibiotic resistance phenomenon.
FAU - Di Lodovico, Silvia
AU  - Di Lodovico S
AD  - Department of Pharmacy, "G. d'Annunzio" University Chieti-Pescara, Chieti, Italy.
FAU - Napoli, Edoardo
AU  - Napoli E
AUID- ORCID: 0000-0003-4281-3256
AD  - Institute of Biomolecular Chemistry, National Research Council ICB-CNR, Catania, 
      Italy.
FAU - Di Campli, Emanuela
AU  - Di Campli E
AUID- ORCID: 0000-0001-5311-2848
AD  - Department of Pharmacy, "G. d'Annunzio" University Chieti-Pescara, Chieti, Italy.
FAU - Di Fermo, Paola
AU  - Di Fermo P
AD  - Department of Pharmacy, "G. d'Annunzio" University Chieti-Pescara, Chieti, Italy.
FAU - Gentile, Davide
AU  - Gentile D
AD  - Institute of Biomolecular Chemistry, National Research Council ICB-CNR, Catania, 
      Italy.
FAU - Ruberto, Giuseppe
AU  - Ruberto G
AD  - Institute of Biomolecular Chemistry, National Research Council ICB-CNR, Catania, 
      Italy.
FAU - Nostro, Antonia
AU  - Nostro A
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, Messina, Italy.
FAU - Marini, Emanuela
AU  - Marini E
AD  - Unit of Microbiology, Department of Biomedical Sciences and Public Health, 
      Polytechnic University of Marche, Ancona, Italy.
FAU - Cellini, Luigina
AU  - Cellini L
AUID- ORCID: 0000-0003-4068-9124
AD  - Department of Pharmacy, "G. d'Annunzio" University Chieti-Pescara, Chieti, Italy.
FAU - Di Giulio, Mara
AU  - Di Giulio M
AUID- ORCID: 0000-0002-8375-9404
AD  - Department of Pharmacy, "G. d'Annunzio" University Chieti-Pescara, Chieti, Italy. 
      mara.digiulio@unich.it.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190315
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Anti-Infective Agents)
RN  - 6GNT3Y5LMF (Levofloxacin)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - Anti-Infective Agents/pharmacology
MH  - Biofilms/drug effects
MH  - Drug Resistance, Bacterial/drug effects
MH  - Drug Synergism
MH  - Helicobacter Infections/drug therapy
MH  - Helicobacter pylori/*drug effects/metabolism/pathogenicity
MH  - Humans
MH  - Levofloxacin/*pharmacology
MH  - Microbial Sensitivity Tests
MH  - Pistacia/*metabolism
PMC - PMC6420558
COIS- The authors declare no competing interests.
EDAT- 2019/03/16 06:00
MHDA- 2020/10/02 06:00
PMCR- 2019/03/15
CRDT- 2019/03/16 06:00
PHST- 2018/08/30 00:00 [received]
PHST- 2019/02/19 00:00 [accepted]
PHST- 2019/03/16 06:00 [entrez]
PHST- 2019/03/16 06:00 [pubmed]
PHST- 2020/10/02 06:00 [medline]
PHST- 2019/03/15 00:00 [pmc-release]
AID - 10.1038/s41598-019-40991-y [pii]
AID - 40991 [pii]
AID - 10.1038/s41598-019-40991-y [doi]
PST - epublish
SO  - Sci Rep. 2019 Mar 15;9(1):4646. doi: 10.1038/s41598-019-40991-y.