PMID- 30877059 OWN - NLM STAT- MEDLINE DCOM- 20190725 LR - 20190725 IS - 1095-9947 (Electronic) IS - 1050-4648 (Linking) VI - 88 DP - 2019 May TI - Recombinant nodavirus vaccine produced in bacteria and administered without purification elicits humoral immunity and protects European sea bass against infection. PG - 458-463 LID - S1050-4648(19)30161-5 [pii] LID - 10.1016/j.fsi.2019.03.013 [doi] AB - Viral necrosis virus (NNV) or nodavirus causes fish viral encephalopathy and retinopathy worldwide. In some cases, mortalities in aquaculture industry can reach up to 100%, some species being especially sensitive as is the case of European sea bass (Dicentrarchus labrax), one of the main cultured species in the Mediterranean, with the consequent economical loses. Development of new vaccines against NNV is in the spotlight though few researches have focused in European sea bass. In this study we have generated a recombinant NNV (rNNV) vaccine produced in Escherichia coli expressing the capsid protein and administered it to European sea bass juveniles by two different routes (intraperitoneal and oral). The last being considered non-stressful and desired for fish farming of small fish, which in fact are the most affected by NNV. Oral vaccine was composed of feed pellets containing the recombinant whole bacteria, and injected vaccine was composed of recombinant bacteria previously lysed. Our results revealed production of specific anti-NNV IgM following the two vaccination procedures, levels that were further increased in orally-vaccinated group after challenge with NNV. Genes related to interferon (IFN), T-cell and immunoglobulin markers were scarcely regulated in head-kidney (HK), gut or brain. Vaccination by either route elicited a relative survival response of 100% after NNV challenge. To our knowledge, this is the first report of a recombinant vaccine followed by no purification steps which resulted in a complete protection in European sea bass when challenged with NNV. CI - Copyright (c) 2019 Elsevier Ltd. All rights reserved. FAU - Gonzalez-Silvera, Daniel AU - Gonzalez-Silvera D AD - Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain. FAU - Guardiola, Francisco A AU - Guardiola FA AD - Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain; Centro Interdisciplinar de Investigacao Marinha e Ambiental (CIIMAR), University of Porto, Terminal de Cruzeiros Do Porto de Leixoes, Av. General Norton de Matos s/n, 4450-208, Porto, Portugal. FAU - Espinosa, Cristobal AU - Espinosa C AD - Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain. FAU - Chaves-Pozo, Elena AU - Chaves-Pozo E AD - Centro Oceanografico de Murcia, Instituto Espanol de Oceanografia (IEO), Carretera de la Azohia s/n, Puerto de Mazarron, 30860, Murcia, Spain. FAU - Esteban, M Angeles AU - Esteban MA AD - Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain. FAU - Cuesta, Alberto AU - Cuesta A AD - Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain. Electronic address: alcuesta@um.es. LA - eng PT - Journal Article DEP - 20190313 PL - England TA - Fish Shellfish Immunol JT - Fish & shellfish immunology JID - 9505220 RN - 0 (Antibodies, Viral) RN - 0 (Vaccines, Synthetic) RN - 0 (Viral Vaccines) MH - Administration, Oral MH - Animals MH - Antibodies, Viral/blood MH - Aquaculture MH - Bass/*immunology/virology MH - Escherichia coli/genetics MH - Fish Diseases/immunology/*prevention & control MH - *Immunity, Humoral MH - Injections, Intraperitoneal MH - *Nodaviridae MH - RNA Virus Infections/prevention & control/*veterinary MH - Vaccination/veterinary MH - Vaccines, Synthetic/immunology MH - Viral Vaccines/*immunology OTO - NOTNLM OT - European sea bass OT - Immunity OT - Nodavirus OT - Oral vaccines EDAT- 2019/03/17 06:00 MHDA- 2019/07/26 06:00 CRDT- 2019/03/17 06:00 PHST- 2018/12/03 00:00 [received] PHST- 2019/03/06 00:00 [revised] PHST- 2019/03/08 00:00 [accepted] PHST- 2019/03/17 06:00 [pubmed] PHST- 2019/07/26 06:00 [medline] PHST- 2019/03/17 06:00 [entrez] AID - S1050-4648(19)30161-5 [pii] AID - 10.1016/j.fsi.2019.03.013 [doi] PST - ppublish SO - Fish Shellfish Immunol. 2019 May;88:458-463. doi: 10.1016/j.fsi.2019.03.013. Epub 2019 Mar 13.