PMID- 30879321
OWN - NLM
STAT- MEDLINE
DCOM- 20190726
LR  - 20190726
IS  - 0001-723X (Print)
IS  - 0001-723X (Linking)
VI  - 63
IP  - 1
DP  - 2019
TI  - Phospholipase C signaling is involved in porcine reproductive and respiratory 
      syndrome virus infection in cell cultures.
PG  - 117-120
LID - 10.4149/av_2019_115 [doi]
AB  - The phospholipase C (PLC) is a family of kinases that hydrolyze 
      phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] to generate two second 
      messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), which 
      stimulate distinct downstream signaling. Recently, it has been reported that PLC 
      signaling is activated by multiple viruses for efficient replication and the 
      virus-induced inflammatory response. In this study, we demonstrated that 
      PLC-specific inhibitor U73122 strongly suppressed porcine reproductive and 
      respiratory syndrome virus (PRRSV) productive infection in cell cultures. The 
      inhibitor affected both viral post-binding cell entry and post-entry processes. 
      The virus infection led to an early transient activation of PLCgamma-1 at 0.5 h 
      post-infection (hpi), and sustained event at a stage from 4 to 16 hpi in MARC-145 
      cells. In addition, U73122 inhibited the activation of p38 MAPK signaling 
      stimulated by PRRSV infection, suggesting that PLC signaling may be associated 
      with the virus infection-induced inflammatory response. Taken together, these 
      studies suggested that PLC signaling played an important role in PRRSV infection 
      or pathogenesis. Keywords: PRRSV; U73122; phospholipase C; PLCgamma-1.
FAU - Yuan, W F
AU  - Yuan WF
FAU - Chen, Q
AU  - Chen Q
FAU - Gao, X T
AU  - Gao XT
FAU - Zheng, Z M
AU  - Zheng ZM
FAU - Jia, H
AU  - Jia H
FAU - Zhu, H F
AU  - Zhu HF
FAU - Xin, T
AU  - Xin T
FAU - Sui, X K
AU  - Sui XK
FAU - Li, M
AU  - Li M
FAU - Hou, S H
AU  - Hou SH
FAU - Guo, X Y
AU  - Guo XY
LA  - eng
PT  - Journal Article
PL  - Slovakia
TA  - Acta Virol
JT  - Acta virologica
JID - 0370401
RN  - 0 (Estrenes)
RN  - 0 (Phosphodiesterase Inhibitors)
RN  - 0 (Pyrrolidinones)
RN  - 112648-68-7 
      (1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione)
RN  - EC 3.1.4.- (Type C Phospholipases)
MH  - Animals
MH  - Cell Line
MH  - Estrenes/pharmacology
MH  - Phosphodiesterase Inhibitors/pharmacology
MH  - *Porcine Reproductive and Respiratory Syndrome/physiopathology
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Pyrrolidinones/pharmacology
MH  - Signal Transduction
MH  - Swine
MH  - *Type C Phospholipases/antagonists & inhibitors/physiology
MH  - Virus Internalization/drug effects
EDAT- 2019/03/19 06:00
MHDA- 2019/07/28 06:00
CRDT- 2019/03/19 06:00
PHST- 2019/03/19 06:00 [entrez]
PHST- 2019/03/19 06:00 [pubmed]
PHST- 2019/07/28 06:00 [medline]
AID - 10.4149/av_2019_115 [doi]
PST - ppublish
SO  - Acta Virol. 2019;63(1):117-120. doi: 10.4149/av_2019_115.