PMID- 30879817
OWN - NLM
STAT- MEDLINE
DCOM- 20190606
LR  - 20211204
IS  - 1525-3198 (Electronic)
IS  - 0022-0302 (Linking)
VI  - 102
IP  - 5
DP  - 2019 May
TI  - Methionine supply during the periparturient period enhances insulin signaling, 
      amino acid transporters, and mechanistic target of rapamycin pathway proteins in 
      adipose tissue of Holstein cows.
PG  - 4403-4414
LID - S0022-0302(19)30259-0 [pii]
LID - 10.3168/jds.2018-15738 [doi]
AB  - Enhanced postruminal supply of Met during the periparturient period increases dry 
      matter intake and milk yield. In nonruminants, adipose tissue is responsive to AA 
      supply, and can use AA as fuels or for protein synthesis regulated in part via 
      insulin and mechanistic target of rapamycin (mTOR) signaling. Whether enhancing 
      supply of Met has an effect on insulin and mTOR pathways in adipose tissue in 
      peripartal cows is unknown. Multiparous Holstein cows were assigned from -28 to 
      60 d relative to parturition to a basal diet (control; 1.47 Mcal/kg of dry matter 
      and 15.3% crude protein prepartum; 1.67 Mcal/kg and 17.7% crude protein 
      postpartum) or the control plus ethyl-cellulose rumen-protected Met (RPM). The 
      RPM was fed individually at a rate of 0.09% of dry matter intake prepartum and 
      0.10% postpartum. Subcutaneous adipose tissue harvested at -10, 10, and 30 d 
      relative to parturition (days in milk) was used for quantitative PCR and Western 
      blotting. A glucose tolerance test was performed at -12 and 12 d in milk to 
      evaluate insulin sensitivity. Area under the curve for glucose in the pre- and 
      postpartum tended to be smaller in cows fed Met. Enhanced Met supply led to 
      greater overall mRNA abundance of Gln (SLC38A1), Glu (SLC1A1), l-type AA (Met, 
      Leu, Val, Phe; SLC3A2), small zwitterionic alpha-AA (SLC36A1), and neutral AA 
      (SLC1A5) transporters. Abundance of AKT1, RPS6KB1, and EIF4EBP1 was also 
      upregulated in response to Met. A diet x day interaction was observed for protein 
      abundance of insulin receptor due to Met cows having lower values at 30 d 
      postpartum compared with controls. The diet x day interaction was significant for 
      hormone-sensitive lipase due to Met cows having greater abundance at 10 d 
      postpartum compared with controls. Enhanced Met supply upregulated protein 
      abundance of insulin-responsive proteins phosphorylated (p)-AKT, peroxisome 
      proliferator-activated receptor gamma, and fatty acid synthase. Overall abundance 
      of solute carrier family 2 member 4 tended to be greater in cows fed Met. A diet 
      x day interaction was observed for mTOR protein abundance due to greater values 
      for RPM cows at 30 d postpartum compared with controls. Enhanced RPM supply 
      upregulated overall protein abundance of solute carrier family 1 member 3, 
      p-mTOR, and ribosomal protein S6. Overall, data indicate that mTOR and insulin 
      signaling pathways in adipose tissue adapt to the change in physiologic state 
      during the periparturient period. Further studies should be done to clarify 
      whether the activation of p-AKT or increased availability of AA leads to the 
      activation of mTOR.
CI  - The Authors. Published by FASS Inc. and Elsevier Inc. on behalf of the American 
      Dairy Science Association(R). This is an open access article under the CC BY-NC-ND 
      license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
FAU - Liang, Y
AU  - Liang Y
AD  - Department of Animal Sciences and Division of Nutritional Sciences, University of 
      Illinois, Urbana 61801.
FAU - Batistel, F
AU  - Batistel F
AD  - Department of Animal Sciences and Division of Nutritional Sciences, University of 
      Illinois, Urbana 61801.
FAU - Parys, C
AU  - Parys C
AD  - Evonik Nutrition & Care GmbH, Hanau-Wolfgang, 63457, Germany.
FAU - Loor, J J
AU  - Loor JJ
AD  - Department of Animal Sciences and Division of Nutritional Sciences, University of 
      Illinois, Urbana 61801. Electronic address: jloor@illinois.edu.
LA  - eng
PT  - Journal Article
DEP - 20190314
PL  - United States
TA  - J Dairy Sci
JT  - Journal of dairy science
JID - 2985126R
RN  - 0 (Amino Acid Transport Systems)
RN  - 0 (Insulin)
RN  - AE28F7PNPL (Methionine)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Adipose Tissue/metabolism
MH  - Amino Acid Transport Systems/*metabolism
MH  - Animals
MH  - Cattle/*physiology
MH  - Insulin/*physiology
MH  - Methionine/administration & dosage/*metabolism
MH  - Peripartum Period/*physiology
MH  - Random Allocation
MH  - Signal Transduction/physiology
MH  - TOR Serine-Threonine Kinases/*metabolism
OTO - NOTNLM
OT  - amino acid transporter
OT  - insulin response
OT  - mechanistic target of rapamycin
OT  - transition period
EDAT- 2019/03/19 06:00
MHDA- 2019/06/07 06:00
CRDT- 2019/03/19 06:00
PHST- 2018/09/22 00:00 [received]
PHST- 2019/01/11 00:00 [accepted]
PHST- 2019/03/19 06:00 [pubmed]
PHST- 2019/06/07 06:00 [medline]
PHST- 2019/03/19 06:00 [entrez]
AID - S0022-0302(19)30259-0 [pii]
AID - 10.3168/jds.2018-15738 [doi]
PST - ppublish
SO  - J Dairy Sci. 2019 May;102(5):4403-4414. doi: 10.3168/jds.2018-15738. Epub 2019 
      Mar 14.