PMID- 30881472
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1741-427X (Print)
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Linking)
VI  - 2019
DP  - 2019
TI  - The Tissue Distribution of Four Major Coumarins after Oral Administration of 
      Angelicae Pubescentis Radix Extract to Rats Using Ultra-High-Performance Liquid 
      Chromatography.
PG  - 2365697
LID - 10.1155/2019/2365697 [doi]
LID - 2365697
AB  - Angelicae pubescentis radix (APR) is widely applied in treating rheumatoid 
      arthritis in China. Coumarins are the major active compounds of APR extract 
      including columbianetin, columbianetin acetate, osthole, and columbianadin. The 
      in vivo behavior of the four major coumarins of APR has not been systematically 
      reported. A feasible and reliable ultra-performance liquid chromatography (UPLC) 
      method was established and validated for the quantification of the above four 
      coumarins in rat various tissues (including heart, liver, spleen, lung, kidney, 
      uterus, ovary, and muscle) after oral administration of APR extract. The 
      separation was implemented on a Waters ACQUITY BEH C18 column (4.6 mm x 100 mm, 
      1.7 mum) with gradient mobile phase comprising acetonitrile-water (with 1mM formic 
      acid) at a flow rate of 0.3 mL/min. The tissue homogenate samples were prepared 
      by liquid-liquid extraction with ethyl acetate. The calibration curves were 
      linear in the range of 1.6-20000 ng/mL for four coumarins with the lower limit of 
      quantitation of 1.6 ng/mL in rat tissues. The intraday and interday precisions 
      and recoveries were all within 80-100% with the relative standard deviations 
      (RSDs) which were all less than 10.9%. The method was successfully applied to the 
      tissue distribution research after oral administration of 6.0 g/kg APR extract to 
      rat. The results revealed that the tissues distributions of four coumarins were 
      in the liver, followed by the ovary, uterus, kidney, lung, heart, spleen, and 
      muscle.
FAU - Ge, Yuanyuan
AU  - Ge Y
AD  - Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin 
      University of Traditional Chinese Medicine, Tianjin, 300193, China.
AD  - Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of 
      Traditional Chinese Medicine, Tianjin, 300193, China.
FAU - Chen, Shujing
AU  - Chen S
AD  - Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin 
      University of Traditional Chinese Medicine, Tianjin, 300193, China.
AD  - Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of 
      Traditional Chinese Medicine, Tianjin, 300193, China.
FAU - Luo, Qian
AU  - Luo Q
AD  - Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin 
      University of Traditional Chinese Medicine, Tianjin, 300193, China.
AD  - Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of 
      Traditional Chinese Medicine, Tianjin, 300193, China.
FAU - Wang, Chun-Peng
AU  - Wang CP
AD  - Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin 
      University of Traditional Chinese Medicine, Tianjin, 300193, China.
AD  - Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of 
      Traditional Chinese Medicine, Tianjin, 300193, China.
FAU - Hao, Jia
AU  - Hao J
AD  - Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin 
      University of Traditional Chinese Medicine, Tianjin, 300193, China.
AD  - Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of 
      Traditional Chinese Medicine, Tianjin, 300193, China.
FAU - He, Jun
AU  - He J
AUID- ORCID: 0000-0002-3781-9234
AD  - Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin 
      University of Traditional Chinese Medicine, Tianjin, 300193, China.
AD  - Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of 
      Traditional Chinese Medicine, Tianjin, 300193, China.
FAU - Chen, Xiaopeng
AU  - Chen X
AUID- ORCID: 0000-0001-7713-1680
AD  - Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin 
      University of Traditional Chinese Medicine, Tianjin, 300193, China.
AD  - Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of 
      Traditional Chinese Medicine, Tianjin, 300193, China.
FAU - Yang, Xuejing
AU  - Yang X
AD  - Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin 
      University of Traditional Chinese Medicine, Tianjin, 300193, China.
AD  - School of Pharmacy, Harbin University of Commerce, Harbin 150076, China.
FAU - Li, Jin
AU  - Li J
AUID- ORCID: 0000-0003-2829-1631
AD  - Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of 
      Traditional Chinese Medicine, Tianjin, 300193, China.
AD  - Key Laboratory of Formula of Traditional Chinese Medicine (Tianjin University of 
      Traditional Chinese Medicine), Ministry of Education, Tianjin, 300193, China.
FAU - Chang, Yan-Xu
AU  - Chang YX
AUID- ORCID: 0000-0002-4198-374X
AD  - Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin 
      University of Traditional Chinese Medicine, Tianjin, 300193, China.
AD  - Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of 
      Traditional Chinese Medicine, Tianjin, 300193, China.
AD  - Key Laboratory of Formula of Traditional Chinese Medicine (Tianjin University of 
      Traditional Chinese Medicine), Ministry of Education, Tianjin, 300193, China.
LA  - eng
PT  - Journal Article
DEP - 20190207
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC6383424
EDAT- 2019/03/19 06:00
MHDA- 2019/03/19 06:01
PMCR- 2019/02/07
CRDT- 2019/03/19 06:00
PHST- 2018/11/09 00:00 [received]
PHST- 2018/12/31 00:00 [revised]
PHST- 2019/01/14 00:00 [accepted]
PHST- 2019/03/19 06:00 [entrez]
PHST- 2019/03/19 06:00 [pubmed]
PHST- 2019/03/19 06:01 [medline]
PHST- 2019/02/07 00:00 [pmc-release]
AID - 10.1155/2019/2365697 [doi]
PST - epublish
SO  - Evid Based Complement Alternat Med. 2019 Feb 7;2019:2365697. doi: 
      10.1155/2019/2365697. eCollection 2019.