PMID- 30882002
OWN - NLM
STAT- MEDLINE
DCOM- 20190530
LR  - 20200225
IS  - 2314-7156 (Electronic)
IS  - 2314-8861 (Print)
IS  - 2314-7156 (Linking)
VI  - 2019
DP  - 2019
TI  - Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human 
      Phagocytic Cells against Paracoccidioides brasiliensis.
PG  - 1529189
LID - 10.1155/2019/1529189 [doi]
LID - 1529189
AB  - Paracoccidioidomycosis (PCM), a systemic mycosis endemic in Latin America, occurs 
      after inhalation of mycelial components of Paracoccidioides spp. When the fungus 
      reaches the lungs and interacts with the alveolar macrophages and other cells, 
      phagocytic cells such as neutrophils and monocytes are immediately recruited to 
      the injured site. The interaction between surface molecules of pathogens and 
      homologous receptors, present on the surface membrane of phagocytes, modulates 
      the innate immune cell activation. Studies have shown the importance of fungal 
      recognition by the Dectin-1 receptor, which can induce a series of cellular 
      protective responses against fungi. The objective of the present study was to 
      evaluate Dectin-1 receptor expression and the effector mechanisms of human 
      monocytes and neutrophils activated or not with different cytokines, such as 
      IFN-gamma, TNF-alpha, and GM-CSF, followed by the challenge with Paracoccidioides 
      brasiliensis (P. brasiliensis or Pb265). Therefore, analysis of Dectin-1 receptor 
      expression was done by flow cytometry whereas the effector mechanisms were 
      evaluated by fungal recovery by colony-forming unit (CFU) counting and hydrogen 
      peroxide (H(2)O(2)) production. Our results showed that, after treatment with 
      IFN-gamma, TNF-alpha, and GM-CSF and challenge with Pb265, cells, especially monocytes, 
      demonstrated an increase in Dectin-1 expression. Both types of cells treated with 
      the cytokines exhibited a decreased fungal recovery and, conversely, an increased 
      production of H(2)O(2). However, when cultures were treated with an anti-Dectin-1 
      monoclonal antibody, to block the P. brasiliensis binding, a decrease in H(2)O(2) 
      production and an increase in fungal recovery were detected. This effect was 
      observed in all cultures treated with the specific monoclonal antibody. These 
      results show the involvement of the Dectin-1 receptor in fungal recognition and 
      its consequent participation in the induction of the killing mechanisms against 
      P. brasiliensis.
FAU - de Quaglia E Silva, Juliana Carvalho
AU  - de Quaglia E Silva JC
AD  - Sao Paulo State University (UNESP), Medical School of Botucatu, Laboratory of 
      Immunopathology and Infectious Agents - LIAI, UNIPEX - Experimental Research 
      Unity, Sector 5, Botucatu SP, Brazil.
FAU - Della Coletta, Amanda Manoel
AU  - Della Coletta AM
AD  - Sao Paulo State University (UNESP), Medical School of Botucatu, Laboratory of 
      Immunopathology and Infectious Agents - LIAI, UNIPEX - Experimental Research 
      Unity, Sector 5, Botucatu SP, Brazil.
FAU - Gardizani, Taiane Priscila
AU  - Gardizani TP
AD  - Sao Paulo State University (UNESP), Medical School of Botucatu, Laboratory of 
      Immunopathology and Infectious Agents - LIAI, UNIPEX - Experimental Research 
      Unity, Sector 5, Botucatu SP, Brazil.
FAU - Romagnoli, Graziela Gorete
AU  - Romagnoli GG
AD  - Sao Paulo State University (UNESP), Institute of Biosciences, Department of 
      Microbiology and Immunology, Botucatu SP, Brazil.
FAU - Kaneno, Ramon
AU  - Kaneno R
AD  - Sao Paulo State University (UNESP), Institute of Biosciences, Department of 
      Microbiology and Immunology, Botucatu SP, Brazil.
FAU - Dias-Melicio, Luciane Alarcao
AU  - Dias-Melicio LA
AUID- ORCID: 0000-0001-9254-2074
AD  - Sao Paulo State University (UNESP), Medical School of Botucatu, Laboratory of 
      Immunopathology and Infectious Agents - LIAI, UNIPEX - Experimental Research 
      Unity, Sector 5, Botucatu SP, Brazil.
AD  - Sao Paulo State University (UNESP), Medical School of Botucatu, Department of 
      Pathology, Botucatu SP, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20190206
PL  - Egypt
TA  - J Immunol Res
JT  - Journal of immunology research
JID - 101627166
RN  - 0 (CLEC7A protein, human)
RN  - 0 (Cytokines)
RN  - 0 (Lectins, C-Type)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Colony Count, Microbial
MH  - Cytokines/*pharmacology
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Hydrogen Peroxide/analysis
MH  - Interferon-gamma/pharmacology
MH  - Lectins, C-Type/genetics/*metabolism
MH  - Male
MH  - Monocytes/drug effects/immunology
MH  - Neutrophils/drug effects/immunology
MH  - Paracoccidioides
MH  - Paracoccidioidomycosis/*immunology
MH  - Phagocytes/drug effects/*immunology
PMC - PMC6381556
EDAT- 2019/03/19 06:00
MHDA- 2019/05/31 06:00
PMCR- 2019/02/06
CRDT- 2019/03/19 06:00
PHST- 2018/08/01 00:00 [received]
PHST- 2018/12/20 00:00 [accepted]
PHST- 2019/03/19 06:00 [entrez]
PHST- 2019/03/19 06:00 [pubmed]
PHST- 2019/05/31 06:00 [medline]
PHST- 2019/02/06 00:00 [pmc-release]
AID - 10.1155/2019/1529189 [doi]
PST - epublish
SO  - J Immunol Res. 2019 Feb 6;2019:1529189. doi: 10.1155/2019/1529189. eCollection 
      2019.