PMID- 30886127
OWN - NLM
STAT- MEDLINE
DCOM- 20200330
LR  - 20211023
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Print)
IS  - 1040-4651 (Linking)
VI  - 31
IP  - 4
DP  - 2019 Apr
TI  - PRX9 and PRX40 Are Extensin Peroxidases Essential for Maintaining Tapetum and 
      Microspore Cell Wall Integrity during Arabidopsis Anther Development.
PG  - 848-861
LID - 10.1105/tpc.18.00907 [doi]
AB  - Pollen and microspore development are essential steps in the life cycle of all 
      land plants that generate male gametes. Within flowering plants, pollen 
      development occurs inside of the anther. Here, we report the identification of 
      two class III peroxidase-encoding genes, PEROXIDASE9 (PRX9) and PRX40, that are 
      genetically redundant and essential for proper anther and pollen development in 
      Arabidopsis (Arabidopsis thaliana). Arabidopsis double mutants devoid of 
      functional PRX9 and PRX40 are male sterile. The mutant anthers display swollen, 
      hypertrophic tapetal cells and pollen grains, suggesting disrupted cell wall 
      integrity. These phenotypes lead to nearly 100%-penetrant pollen degeneration 
      upon anther maturation. Using immunochemical and biochemical approaches, we show 
      that PRX9 and PRX40 likely cross-link extensins to contribute to tapetal cell 
      wall integrity during anther development. This work suggests that PRX9 and PRX40 
      encode Arabidopsis extensin peroxidases and highlights the importance of extensin 
      cross-linking during pollen development.
CI  - (c) 2019 American Society of Plant Biologists. All rights reserved.
FAU - Jacobowitz, Joseph R
AU  - Jacobowitz JR
AUID- ORCID: 0000-0001-6289-8889
AD  - Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
AD  - Department of Biology, Massachusetts Institute of Technology, Cambridge, 
      Massachusetts, 02139.
FAU - Doyle, William C
AU  - Doyle WC
AUID- ORCID: 0000-0002-2777-8037
AD  - Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
FAU - Weng, Jing-Ke
AU  - Weng JK
AUID- ORCID: 0000-0003-3059-0075
AD  - Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142 
      wengj@wi.mit.edu.
AD  - Department of Biology, Massachusetts Institute of Technology, Cambridge, 
      Massachusetts, 02139.
LA  - eng
GR  - T32 GM007287/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20190318
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Arabidopsis Proteins)
SB  - IM
MH  - Arabidopsis/genetics/*metabolism/*physiology
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Cell Wall/genetics/metabolism/physiology
MH  - Gene Expression Regulation, Plant/genetics/physiology
MH  - Plants, Genetically Modified/genetics/*metabolism/physiology
PMC - PMC6501601
EDAT- 2019/03/20 06:00
MHDA- 2020/03/31 06:00
PMCR- 2019/03/18
CRDT- 2019/03/20 06:00
PHST- 2018/11/28 00:00 [received]
PHST- 2019/02/06 00:00 [revised]
PHST- 2019/03/13 00:00 [accepted]
PHST- 2019/03/20 06:00 [pubmed]
PHST- 2020/03/31 06:00 [medline]
PHST- 2019/03/20 06:00 [entrez]
PHST- 2019/03/18 00:00 [pmc-release]
AID - tpc.18.00907 [pii]
AID - 201800907DR1 [pii]
AID - 10.1105/tpc.18.00907 [doi]
PST - ppublish
SO  - Plant Cell. 2019 Apr;31(4):848-861. doi: 10.1105/tpc.18.00907. Epub 2019 Mar 18.