PMID- 30887556 OWN - NLM STAT- MEDLINE DCOM- 20200831 LR - 20200831 IS - 1097-4644 (Electronic) IS - 0730-2312 (Linking) VI - 120 IP - 8 DP - 2019 Aug TI - Elevated expression of ICAM-1 in synovium is associated with early inflammatory response for cartilage degeneration in type 2 diabetes mellitus. PG - 13177-13186 LID - 10.1002/jcb.28592 [doi] AB - Type 2 diabetes mellitus (T2DM) is increasingly being recognized as an independent risk factor for the onset and progression of osteoarthritis (OA). Extensive studies have focused on the contribution of obesity (excessive mechanical stress), comorbidity frequently found in T2DM, to cartilage destruction during OA development. However, a little is known about how diabetes-related inflammation may affect the local cartilage in a diabetic objective. In the present study, we were able to establish a T2DM rat model using a combination of a low dose of streptozotocin with high-fat and high-sugar diet. Although the cartilage integrity was comparable between the control and T2DM groups, the expression of matrix metalloproteinases-13 (MMP-13) was significantly upregulated in T2DM, indicating the initiation of an early cascade of cartilage degeneration. In parallel, an obvious alteration of subchondral bone remodeling (inhibition of bone formation) was observed, as evidenced by the reduction of osterix-expressing positive cells. Moreover, we demonstrated that the expression of intercellular adhesion molecule-1 (ICAM-1) in the serum and synovium of T2DM rats was elevated, accompanied by an increase of synovitis score. We also noticed that the number of F4/80-positive macrophage cells was significantly increased in the T2DM group. Mechanistically, the expression of ICAM-1 in fibroblast-like synoviocytes can be triggered by glucose and interleukin-1beta, which are the two important factors within the joint of T2DM. Given that MMP-13 expression was significantly upregulated in the T2DM cartilage, and that ICAM-1-mediated filtration of macrophage was associated with synovitis, we propose that ICAM-1 is essential for triggering a vicious cycle of inflammation within the joint, which together subsequently drivers the cartilage degradation. CI - (c) 2019 Wiley Periodicals, Inc. FAU - Gui, Tao AU - Gui T AD - Department of Bone and Joint Surgery, Institute of Orthopedic Diseases, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China. FAU - Lin, Yong-Kai AU - Lin YK AD - Department of Endocrinology, The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China. FAU - Huan, Song-Wei AU - Huan SW AD - Department of Bone and Joint Surgery, Institute of Orthopedic Diseases, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China. FAU - Li, Yu-Hang AU - Li YH AD - Department of Bone and Joint Surgery, Institute of Orthopedic Diseases, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China. FAU - Wang, Bao-Hua AU - Wang BH AD - Department of Endocrinology, The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China. FAU - Yang, Jie AU - Yang J AD - Department of Bone and Joint Surgery, Institute of Orthopedic Diseases, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China. FAU - Gu, Rong-He AU - Gu RH AD - Department of Orthopedics, The First People's Hospital of Nanning, Nanning, Guangxi, China. FAU - Liu, Qing AU - Liu Q AD - Department of Endocrinology, The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China. FAU - Li, Zhen-Yan AU - Li ZY AD - Department of Bone and Joint Surgery, Institute of Orthopedic Diseases, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China. FAU - Li, Sai-Mei AU - Li SM AD - Department of Endocrinology, The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China. FAU - Chen, Yuan-Feng AU - Chen YF AD - Department of Bone and Joint Surgery, Institute of Orthopedic Diseases, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China. FAU - Zhang, Huan-Tian AU - Zhang HT AUID- ORCID: 0000-0002-7391-436X AD - Department of Bone and Joint Surgery, Institute of Orthopedic Diseases, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China. FAU - Zha, Zhen-Gang AU - Zha ZG AD - Department of Bone and Joint Surgery, Institute of Orthopedic Diseases, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190319 PL - United States TA - J Cell Biochem JT - Journal of cellular biochemistry JID - 8205768 RN - 0 (Cytokines) RN - 126547-89-5 (Intercellular Adhesion Molecule-1) SB - IM MH - Animals MH - Cytokines/metabolism MH - Diabetes Mellitus, Type 2/*metabolism MH - Immunohistochemistry MH - Inflammation/*immunology/*metabolism MH - Intercellular Adhesion Molecule-1/*metabolism MH - Male MH - Osteoarthritis/immunology/metabolism MH - Rats MH - Rats, Sprague-Dawley MH - Real-Time Polymerase Chain Reaction MH - Synovial Membrane/*immunology/*metabolism MH - X-Ray Microtomography OTO - NOTNLM OT - cartilage OT - inflammation OT - intercellular adhesion molecule-1 OT - matrix metalloproteinases-13 OT - synovium OT - type 2 diabetes mellitus EDAT- 2019/03/20 06:00 MHDA- 2020/09/01 06:00 CRDT- 2019/03/20 06:00 PHST- 2018/11/29 00:00 [received] PHST- 2019/02/13 00:00 [revised] PHST- 2019/02/21 00:00 [accepted] PHST- 2019/03/20 06:00 [pubmed] PHST- 2020/09/01 06:00 [medline] PHST- 2019/03/20 06:00 [entrez] AID - 10.1002/jcb.28592 [doi] PST - ppublish SO - J Cell Biochem. 2019 Aug;120(8):13177-13186. doi: 10.1002/jcb.28592. Epub 2019 Mar 19.