PMID- 30888537 OWN - NLM STAT- MEDLINE DCOM- 20190530 LR - 20190530 IS - 1432-5233 (Electronic) IS - 0940-5429 (Linking) VI - 56 IP - 5 DP - 2019 May TI - Understanding the gut-kidney axis among biopsy-proven diabetic nephropathy, type 2 diabetes mellitus and healthy controls: an analysis of the gut microbiota composition. PG - 581-592 LID - 10.1007/s00592-019-01316-7 [doi] AB - AIMS: Type 2 diabetes mellitus (T(2)DM) has a rising prevalence and gut microbiota involvement is increasingly recognized. Diabetic nephropathy (DN) is a major complication of T(2)DM. The aim of the study was to understand the gut-kidney axis by an analysis of gut microbiota composition among biopsy-proven DN, T(2)DM without kidney disease, and healthy control. METHODS: Fecal samples were collected from 14 DNs, 14 age/gender-matched T(2)DMs without renal diseases (DM), 14 age and gender-matched healthy controls (HC) and household contacts (HH) of DM group. The microbiota composition was analyzed by 16sRNA microbial profiling approach. RESULTS: Substantial differences were found in the richness of gut microbiota and the variation of bacteria population in DM compared to HC, and DN compared to DM, respectively. DM could be accurately distinguished from age/gender-matched healthy controls by the variable of genus g_Prevotella_9 (AUC = 0.9), and DN patients could be accurately distinguished from age/gender-matched DM by the variables of two genera (g_Escherichia-Shigella and g_Prevotella_9, AUC = 0.86). The microbiota composition of HH group was close to that of HC group, and was different from DM group. Under the same diet, DM could be more accurately detected by the same genus (g_Prevotella_9, AUC = 0.92). CONCLUSION: Gut microbiota composition was explored to be related to the occurrence of biopsy-proven DN from DM. DM could be distinguished from HC by detecting g_Prevotella_9 level in feces, while DN was different from DM by the variables of g_Escherichia-Shigella and g_Prevotella_9, which potentially contributed to the physiopathological diagnosis of DN from DM. FAU - Tao, Sibei AU - Tao S AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Li, Lingzhi AU - Li L AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Li, Ling AU - Li L AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Liu, Yuan AU - Liu Y AD - Chinese Health Service Management Department, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Ren, Qian AU - Ren Q AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Shi, Min AU - Shi M AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Liu, Jing AU - Liu J AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Jiang, Jing AU - Jiang J AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Ma, Huichao AU - Ma H AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Huang, Zhuo AU - Huang Z AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Xia, Zijing AU - Xia Z AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Pan, Jing AU - Pan J AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Wei, Tiantian AU - Wei T AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Wang, Yan AU - Wang Y AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Li, Peiyun AU - Li P AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Lan, Tian AU - Lan T AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Tang, Xi AU - Tang X AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Zeng, Xiaoxi AU - Zeng X AD - West China Biomedical Big Data Center, Sichuan University, Chengdu, 610041, China. FAU - Lei, Song AU - Lei S AD - Department of Pathology, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Tang, Huairong AU - Tang H AD - Chinese Health Service Management Department, West China Hospital of Sichuan University, Chengdu, 610041, China. FAU - Ma, Liang AU - Ma L AUID- ORCID: 0000-0001-8327-7969 AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. liang_m@scu.edu.cn. AD - Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Beijing, 10000, China. liang_m@scu.edu.cn. FAU - Fu, Ping AU - Fu P AD - Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, 610041, China. fupinghx@163.com. LA - eng GR - 2016YFC1305403/National Key Research & Development Program of China/ GR - 81700634/National Natural Science Foundation of China/ GR - 2016HH0069/International cooperation project funded by Science and Technology Department of Sichuan Province/ PT - Journal Article DEP - 20190319 PL - Germany TA - Acta Diabetol JT - Acta diabetologica JID - 9200299 RN - 0 (DNA, Bacterial) SB - IM MH - Adult MH - Bacteria/genetics/isolation & purification MH - Biopsy MH - DNA, Bacterial/analysis MH - Diabetes Mellitus, Type 2/complications/*microbiology MH - Diabetic Nephropathies/*microbiology/pathology MH - Diet MH - Feces/microbiology MH - Female MH - Gastrointestinal Microbiome/*physiology MH - Humans MH - Kidney/physiopathology MH - Male MH - Middle Aged MH - Prevotella/isolation & purification OTO - NOTNLM OT - Diabetic nephropathy OT - Gut microbiota OT - Gut-kidney axis OT - Type 2 diabetes mellitus EDAT- 2019/03/20 06:00 MHDA- 2019/05/31 06:00 CRDT- 2019/03/20 06:00 PHST- 2018/10/23 00:00 [received] PHST- 2019/02/23 00:00 [accepted] PHST- 2019/03/20 06:00 [pubmed] PHST- 2019/05/31 06:00 [medline] PHST- 2019/03/20 06:00 [entrez] AID - 10.1007/s00592-019-01316-7 [pii] AID - 10.1007/s00592-019-01316-7 [doi] PST - ppublish SO - Acta Diabetol. 2019 May;56(5):581-592. doi: 10.1007/s00592-019-01316-7. Epub 2019 Mar 19.