PMID- 30890499
OWN - NLM
STAT- MEDLINE
DCOM- 20190403
LR  - 20231213
IS  - 1673-4254 (Print)
IS  - 2663-0842 (Electronic)
IS  - 1673-4254 (Linking)
VI  - 39
IP  - 2
DP  - 2019 Feb 28
TI  - [Targeted binding of estradiol with ESR1 promotes proliferation of human 
      chondrocytes in vitro by inhibiting activation of ERK signaling pathway].
PG  - 134-143
LID - 10.12122/j.issn.1673-4254.2019.09.02 [doi]
AB  - OBJECTIVE: To investigate the effect of estradiol (E2)/estrogen receptor 1 (ESR1) 
      on the proliferation of human chondrocytes in vitro and explore the molecular 
      mechanism. METHODS: The Ad-Easy adenovirus packaging system was used to construct 
      and package the ESR1-overexpressing adenovirus Ad-ESR1. Western blotting and qPCR 
      were used to detect the expression of ESR1 protein and mRNA in human chondrocyte 
      C28I2 cells. In the cells treated with different adenoviruses, the effects of E2 
      were tested on the expressions of proteins related with cell autophagy and 
      apoptosis and the phosphorylation of ERK signaling pathway using Western 
      blotting. Immunofluorescence assay was used to observe the intracellular 
      autophagic flow, flow cytometry was performed to analyze the cell apoptosis rate 
      and the cell cycle changes, and qPCR was used to detect the expressions of PCNA, 
      cyclin B1 and cyclin D1 mRNAs. The inhibitory effect of the specific inhibitor of 
      ERK on the expressions of autophagy- and apoptosis-related genes at both the 
      protein and mRNA levels were detected using Western blotting and qPCR. RESULTS: 
      Transfection with the recombinant adenovirus overexpressing ESR1 and E2 treatment 
      of C28I2 cells significantly enhanced the expressions of autophagy-related 
      proteins LC3, ATG7, promoted the colocalization of LC3 and LAMP1 in the 
      cytoplasm, increased the expressions of the proliferation-related marker genes 
      PCNA, cyclin B1 and cyclin D1, and supressed the expressions of cleaved 
      caspase-3, caspase-12 and pERK. RNA interference of ESR1 obviously lowered the 
      expression levels of autophagy-related proteins in C28I2 cells, causing also 
      suppression of the autophagic flow, increments of the expressions of 
      apoptosis-related proteins and pERK, and down-regulated the expressions of the 
      proliferation marker genes. Blocking ERK activation with the ERK inhibitor 
      obviously inhibited the effects of E2/ESR1 on autophagy, proliferationrelated 
      gene expressions and cell apoptosis. CONCLUSIONS: The targeted binding of E2 with 
      ESR1 promotes the proliferation of human chondrocytes in vitro possibly by 
      inhibiting the activation of ERK signaling pathway to promote cell autophagy and 
      induce cell apoptosis.
FAU - Liu, Min
AU  - Liu M
AD  - Department of Cell Biology and Genetics, Core Facility of Development Biology, 
      Basic Medical Science of Chongqing Medical University, Chongqing 400016, China.
FAU - Xie, Weiwei
AU  - Xie W
AD  - Department of Cell Biology and Genetics, Core Facility of Development Biology, 
      Basic Medical Science of Chongqing Medical University, Chongqing 400016, China.
FAU - Zheng, Wei
AU  - Zheng W
AD  - Department of Cell Biology and Genetics, Core Facility of Development Biology, 
      Basic Medical Science of Chongqing Medical University, Chongqing 400016, China.
FAU - Yin, Danyang
AU  - Yin D
AD  - Department of Cell Biology and Genetics, Core Facility of Development Biology, 
      Basic Medical Science of Chongqing Medical University, Chongqing 400016, China.
FAU - Luo, Rui
AU  - Luo R
AD  - Department of Cell Biology and Genetics, Core Facility of Development Biology, 
      Basic Medical Science of Chongqing Medical University, Chongqing 400016, China.
FAU - Guo, Fengjin
AU  - Guo F
AD  - Department of Cell Biology and Genetics, Core Facility of Development Biology, 
      Basic Medical Science of Chongqing Medical University, Chongqing 400016, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (ESR1 protein, human)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (LAMP1 protein, human)
RN  - 0 (Lysosomal Membrane Proteins)
RN  - 0 (MAP1LC3A protein, human)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 6.2.1.45 (ATG7 protein, human)
RN  - EC 6.2.1.45 (Autophagy-Related Protein 7)
SB  - IM
MH  - Adenoviridae/metabolism
MH  - *Apoptosis
MH  - *Autophagy
MH  - Autophagy-Related Protein 7/metabolism
MH  - Cell Line
MH  - *Cell Proliferation
MH  - Chondrocytes/*cytology/metabolism
MH  - Estradiol/*metabolism
MH  - Estrogen Receptor alpha/*metabolism
MH  - Humans
MH  - Lysosomal Membrane Proteins/metabolism
MH  - *MAP Kinase Signaling System
MH  - Microtubule-Associated Proteins/metabolism
MH  - Transfection
PMC - PMC6765635
OTO - NOTNLM
OT  - ERK signaling pathway
OT  - ESR1
OT  - apoptosis
OT  - autophagy
OT  - estradiol
OT  - proliferation
EDAT- 2019/03/21 06:00
MHDA- 2019/04/04 06:00
PMCR- 2019/02/20
CRDT- 2019/03/21 06:00
PHST- 2019/03/21 06:00 [entrez]
PHST- 2019/03/21 06:00 [pubmed]
PHST- 2019/04/04 06:00 [medline]
PHST- 2019/02/20 00:00 [pmc-release]
AID - nfykdxxb-39-2-134 [pii]
AID - 10.12122/j.issn.1673-4254.2019.09.02 [doi]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2019 Feb 28;39(2):134-143. doi: 
      10.12122/j.issn.1673-4254.2019.09.02.