PMID- 30890956
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 10
DP  - 2019
TI  - Ganoderma triterpenes Protect Against Hyperhomocysteinemia Induced 
      Endothelial-Mesenchymal Transition via TGF-beta Signaling Inhibition.
PG  - 192
LID - 10.3389/fphys.2019.00192 [doi]
LID - 192
AB  - Endothelial dysfunction is one of the most important pathological status in 
      hyperhomocysteinemia (HHcy) related cardiovascular diseases. Whereas, the 
      underlying mechanisms have not been fully elucidated yet, concomitant with the 
      absence of effective treatment. The purpose of this study was to explore the main 
      mechanisms involved in HHcy-induced endothelial injury and identify the 
      protective effect of Ganoderma triterpenes (GT). Bovine aortic endothelial cells 
      (BAECs) were applied as in vitro experimental model. The small molecular 
      inhibitors were used to explore the signalings involved in HHcy-induced 
      endothelial injury. The experimental results provided initial evidence that HHcy 
      led to endothelial-mesenchymal transition (EndMT). Meanwhile, TGF-beta/Smad, 
      PI3K/AKT and MAPK pathways were activated in this process, which was demonstrated 
      by pretreatment with TGF-beta RI kinase inhibitor VI SB431542, PI3K inhibitor 
      LY294002, p38 inhibitor SB203580, and ERK inhibitor PD98059. Furthermore, it was 
      found that GT restrained the process of HHcy-induced EndMT via reducing oxidative 
      stress and suppressing fore mentioned pathways with further inhibiting the 
      activity of Snail. These results implicate that there is an untapped potential 
      for GT as a novel therapeutic candidate for HHcy-induced EndMT through 
      alleviating oxidative stress and canonical TGF-beta/Smad and non-Smad dependent 
      signaling pathways.
FAU - He, Jinzhao
AU  - He J
AD  - Key Laboratory of Molecular Cardiovascular Sciences, State Key Laboratory of 
      Natural and Biomimetic Drugs, Department of Pharmacology, School of Basic Medical 
      Sciences, Peking University, Beijing, China.
FAU - Sun, Yi
AU  - Sun Y
AD  - Key Laboratory of Molecular Cardiovascular Sciences, State Key Laboratory of 
      Natural and Biomimetic Drugs, Department of Pharmacology, School of Basic Medical 
      Sciences, Peking University, Beijing, China.
FAU - Jia, Yingli
AU  - Jia Y
AD  - Key Laboratory of Molecular Cardiovascular Sciences, State Key Laboratory of 
      Natural and Biomimetic Drugs, Department of Pharmacology, School of Basic Medical 
      Sciences, Peking University, Beijing, China.
FAU - Geng, Xiaoqiang
AU  - Geng X
AD  - Key Laboratory of Molecular Cardiovascular Sciences, State Key Laboratory of 
      Natural and Biomimetic Drugs, Department of Pharmacology, School of Basic Medical 
      Sciences, Peking University, Beijing, China.
FAU - Chen, Ruoyun
AU  - Chen R
AD  - State Key Laboratory of Bioactive Substance and Function of Natural Medicines, 
      Chinese Academy of Medical Sciences, Institute of Materia Medica, Peking Union 
      Medical College, Beijing, China.
FAU - Zhou, Hong
AU  - Zhou H
AD  - Key Laboratory of Molecular Cardiovascular Sciences, State Key Laboratory of 
      Natural and Biomimetic Drugs, Department of Pharmacology, School of Basic Medical 
      Sciences, Peking University, Beijing, China.
FAU - Yang, Baoxue
AU  - Yang B
AD  - Key Laboratory of Molecular Cardiovascular Sciences, State Key Laboratory of 
      Natural and Biomimetic Drugs, Department of Pharmacology, School of Basic Medical 
      Sciences, Peking University, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20190305
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC6412081
OTO - NOTNLM
OT  - Ganoderma triterpenes
OT  - TGF-beta
OT  - endothelial cells
OT  - endothelial-mesenchymal transition
OT  - homocysteine
OT  - oxidative stress
EDAT- 2019/03/21 06:00
MHDA- 2019/03/21 06:01
PMCR- 2019/03/05
CRDT- 2019/03/21 06:00
PHST- 2018/10/29 00:00 [received]
PHST- 2019/02/15 00:00 [accepted]
PHST- 2019/03/21 06:00 [entrez]
PHST- 2019/03/21 06:00 [pubmed]
PHST- 2019/03/21 06:01 [medline]
PHST- 2019/03/05 00:00 [pmc-release]
AID - 10.3389/fphys.2019.00192 [doi]
PST - epublish
SO  - Front Physiol. 2019 Mar 5;10:192. doi: 10.3389/fphys.2019.00192. eCollection 
      2019.