PMID- 30891574
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231006
IS  - 2576-2095 (Electronic)
IS  - 2096-5451 (Print)
IS  - 2576-2095 (Linking)
VI  - 1
IP  - 4
DP  - 2018 Dec
TI  - Reference values for selected hematological, biochemical and physiological 
      parameters of Sprague-Dawley rats at the Animal House, Faculty of Medicine, 
      University of Colombo, Sri Lanka.
PG  - 250-254
LID - 10.1002/ame2.12041 [doi]
AB  - BACKGROUND: Lack of available reference values in a research setting under local 
      conditions can be a drawback for beginners, as the accuracy of data from control 
      samples cannot be checked at the beginning of a research project. This affects 
      comparisons with data from test samples. To avoid these complications in their 
      research projects, beginners tend to have a greater number of animals in the 
      control group compared to test groups in order to have control group measurements 
      within 2 SDs of the mean. METHODS: As non-availability of reference values was a 
      long-felt need, the described project was conducted in order to establish a 
      reference database for selected haematological, biochemical and physiological 
      parameters using apparently healthy Sprague-Dawley rats bred in the Animal House 
      of Faculty of Medicine, University of Colombo (UCFM). RESULTS: Differences in 
      mean values of packed cell volume (PCV), mean corpuscular volume (MCV) and mean 
      corpuscular hemoglobin concentration (MCHC), serum creatinine and blood glucose 
      levels between the two genders were statistically significant. Lipid profile 
      measurements did not differ significantly between genders, but mean and median 
      values of triglycerides (TG) between male and female rats showed a difference of 
      more than 10 mg/dL. The liver enzymes alkaline phosphatase (AP) and aspartate 
      aminotransferase (AST) were also statistically significantly different between 
      sexes. Despite wide variation in mean alanine aminotransferase (ALT) between 
      sexes, the difference was not statistically significant. CONCLUSION: The findings 
      of this project should support to a certain extent the "Reduction" aspect of the 
      3Rs concept of Russell and Burch by reducing the number of Sprague-Dawley rats 
      used in future research projects at UCFM.
FAU - Delwatta, Shehani L
AU  - Delwatta SL
AD  - Faculty of Medical Sciences University of Sri Jayawardanepura Gangodawila 
      Nugegoda Sri Lanka.
FAU - Gunatilake, Mangala
AU  - Gunatilake M
AUID- ORCID: 0000-0002-5753-5167
AD  - Faculty of Medicine University of Colombo Colombo Sri Lanka.
FAU - Baumans, Vera
AU  - Baumans V
AD  - Division of Laboratory Animal Science Utrecht University Utrecht The Netherlands.
FAU - Seneviratne, Melanie D
AU  - Seneviratne MD
AD  - Faculty of Medicine University of Colombo Colombo Sri Lanka.
FAU - Dissanayaka, Manjula L B
AU  - Dissanayaka MLB
AD  - Faculty of Medicine University of Colombo Colombo Sri Lanka.
FAU - Batagoda, Siyani S
AU  - Batagoda SS
AD  - Faculty of Medicine University of Colombo Colombo Sri Lanka.
FAU - Udagedara, Asanga H
AU  - Udagedara AH
AD  - Faculty of Medicine University of Colombo Colombo Sri Lanka.
FAU - Walpola, Prasad B
AU  - Walpola PB
AD  - Faculty of Medicine University of Colombo Colombo Sri Lanka.
LA  - eng
PT  - Journal Article
DEP - 20181121
PL  - United States
TA  - Animal Model Exp Med
JT  - Animal models and experimental medicine
JID - 101726292
PMC - PMC6388088
OTO - NOTNLM
OT  - 3Rs concept
OT  - Sprague-Dawley
OT  - rat
OT  - reference values
COIS- None.
EDAT- 2019/03/21 06:00
MHDA- 2019/03/21 06:01
PMCR- 2018/11/21
CRDT- 2019/03/21 06:00
PHST- 2018/03/05 00:00 [received]
PHST- 2018/09/24 00:00 [revised]
PHST- 2018/10/21 00:00 [accepted]
PHST- 2019/03/21 06:00 [entrez]
PHST- 2019/03/21 06:00 [pubmed]
PHST- 2019/03/21 06:01 [medline]
PHST- 2018/11/21 00:00 [pmc-release]
AID - AME212041 [pii]
AID - 10.1002/ame2.12041 [doi]
PST - epublish
SO  - Animal Model Exp Med. 2018 Nov 21;1(4):250-254. doi: 10.1002/ame2.12041. 
      eCollection 2018 Dec.