PMID- 30893293 OWN - NLM STAT- MEDLINE DCOM- 20190826 LR - 20200225 IS - 1643-3750 (Electronic) IS - 1234-1010 (Print) IS - 1234-1010 (Linking) VI - 25 DP - 2019 Mar 20 TI - Long Noncoding RNA (lncRNA) Small Nucleolar RNA Host Gene 16 (SNHG16) Predicts Poor Prognosis and Sorafenib Resistance in Hepatocellular Carcinoma. PG - 2079-2086 LID - 10.12659/MSM.915541 [doi] AB - BACKGROUND Long noncoding RNAs (lncRNAs) play important roles in cancer development and therapeutic resistance. However, the role of small nucleolar RNA host gene 16 (SNHG16) in the development of hepatocellular carcinoma (HCC) remains largely unknown. MATERIAL AND METHODS In situ hybridization (ISH) staining was performed to detect the expression level of SNHG16 in HCC tissues and adjacent non-cancerous tissues. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the level of SNHG16 in HCC samples, adjacent non-cancerous tissues and HCC cell lines. Transwell assay was performed to investigate the migration and invasion ability of HCC cells. Cell viability assays were performed to determine the ability of proliferation and sorafenib resistance of HCC cells. RESULTS We found that SNHG16 was upregulated in HCC tissues and cell lines and that it was negatively correlated with survival time in HCC patients. Univariate and multivariate analyses revealed that SNHG16 was a significant and independent predictor for the overall survival of HCC patients. Furthermore, downregulation of SNHG16 inhibited proliferation, migration, invasion, and sorafenib resistance in hepatocellular carcinoma cell lines. CONCLUSIONS Our findings revealed that lncRNA SNHG16 could be used as an oncogene to predict the outcome of hepatocellular carcinoma. FAU - Guo, Zhenli AU - Guo Z AD - Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland). AD - Department of Pathology, The First Affiliated Hospital of University of Science and Technology of China, Hefei, Anhui, China (mainland). FAU - Zhang, Ju AU - Zhang J AD - Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland). AD - Department of Laboratory Medicine, The First Affiliated Hospital of University of Science and Technology of China, Hefei, Anhui, China (mainland). FAU - Fan, Lulu AU - Fan L AD - Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland). FAU - Liu, Jiatao AU - Liu J AD - Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland). FAU - Yu, Hanqing AU - Yu H AD - Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland). FAU - Li, Xiaoqiu AU - Li X AD - Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland). FAU - Sun, Guoping AU - Sun G AD - Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland). LA - eng PT - Journal Article DEP - 20190320 PL - United States TA - Med Sci Monit JT - Medical science monitor : international medical journal of experimental and clinical research JID - 9609063 RN - 0 (Antineoplastic Agents) RN - 0 (RNA, Long Noncoding) RN - 0 (RNA, Small Nucleolar) RN - 0 (SNHG16 lncRNA, human) RN - 9ZOQ3TZI87 (Sorafenib) MH - Aged MH - Antineoplastic Agents/pharmacology/therapeutic use MH - Carcinoma, Hepatocellular/*drug therapy/*genetics/pathology MH - Cell Line, Tumor MH - Cell Proliferation/genetics MH - Down-Regulation MH - Drug Resistance, Neoplasm MH - Female MH - Humans MH - In Situ Hybridization MH - Liver Neoplasms/*drug therapy/*genetics/pathology MH - Male MH - Middle Aged MH - Prognosis MH - RNA Interference MH - RNA, Long Noncoding/biosynthesis/*genetics/metabolism MH - RNA, Small Nucleolar/genetics MH - Sorafenib/*pharmacology/therapeutic use MH - Transcriptome PMC - PMC6437716 COIS- Conflict of interest None. EDAT- 2019/03/21 06:00 MHDA- 2019/08/27 06:00 PMCR- 2019/03/20 CRDT- 2019/03/21 06:00 PHST- 2019/03/21 06:00 [entrez] PHST- 2019/03/21 06:00 [pubmed] PHST- 2019/08/27 06:00 [medline] PHST- 2019/03/20 00:00 [pmc-release] AID - 915541 [pii] AID - 10.12659/MSM.915541 [doi] PST - epublish SO - Med Sci Monit. 2019 Mar 20;25:2079-2086. doi: 10.12659/MSM.915541.