PMID- 30893621 OWN - NLM STAT- MEDLINE DCOM- 20190425 LR - 20200615 IS - 1879-1026 (Electronic) IS - 0048-9697 (Print) IS - 0048-9697 (Linking) VI - 669 DP - 2019 Jun 15 TI - Transcriptomic analysis of Anabas testudineus and its defensive mechanisms in response to persistent organic pollutants exposure. PG - 621-630 LID - S0048-9697(19)30952-0 [pii] LID - 10.1016/j.scitotenv.2019.02.440 [doi] AB - The freshwater climbing perch (Anabas testudineus) can tolerate water environments contaminated with persistent organic pollutants (POPs). The mechanisms underlying this tolerance are unknown. We used de novo transcriptomic analysis to investigate the defensive mechanisms of A. testudineus against POPs based on its genetic features and biological responses to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure. Our results revealed a specific expansion of cytochrome P450 (CYP) 3A subfamily, which may be involved in the elimination of certain POPs. In xenobiotic responses, the aryl-hydrocarbon receptor (AhR) pathway represents a critical signaling mechanism, and we characterized four AhR and two AhR nuclear translocator homologs and one AhR repressor (AhRR) gene in A. testudineus. TCDD-induced AhRR and CYP1A mRNA upregulation suggests that negative-feedback regulation of AhR signaling through AhRR helps avoid excessive xenobiotic responses. Furthermore, liver and gill transcriptomic profiles were markedly altered after TCDD exposure, with some of the altered genes being related to common defensive responses reported in other species. Based on the newly identified TCDD-altered genes, several A. testudineus-specific responses are proposed, such as enhanced fatty acid beta-oxidation. The genetic features of CYP3A subfamily and AhR pathway and the TCDD-induced defensive biological processes elucidated here enhance our understanding of A. testudineus defensive responses against POPs. CI - Copyright (c) 2019 Elsevier B.V. All rights reserved. FAU - Zhang, Wanglong AU - Zhang W AD - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center of Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Xie, Heidi Qunhui AU - Xie HQ AD - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center of Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Li, Yunping AU - Li Y AD - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center of Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Jin, Tao AU - Jin T AD - China National Genebank-Shenzhen, BGI-Shenzhen, Shenzhen 518083, China; BGI-Qingdao, Qingdao 266510, China. FAU - Li, Jiao AU - Li J AD - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center of Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Xu, Li AU - Xu L AD - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center of Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Zhou, Zhiguang AU - Zhou Z AD - State Environmental Protection Key Laboratory of Dioxin Pollution Control, National Research Center for Environmental Analysis and Measurement, Beijing 100029, China. FAU - Zhang, Songyan AU - Zhang S AD - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center of Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Ma, Dan AU - Ma D AD - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center of Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Hahn, Mark E AU - Hahn ME AD - Woods Hole Oceanographic Institution (WHOI), Woods Hole, MA 02543, USA; Boston University Superfund Research Program, Boston University, Boston, MA 02118, USA. FAU - Zhao, Bin AU - Zhao B AD - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center of Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: binzhao@rcees.ac.cn. LA - eng GR - P42 ES007381/ES/NIEHS NIH HHS/United States PT - Journal Article DEP - 20190228 PL - Netherlands TA - Sci Total Environ JT - The Science of the total environment JID - 0330500 RN - 0 (Polychlorinated Dibenzodioxins) RN - 0 (RNA, Messenger) RN - 0 (Receptors, Aryl Hydrocarbon) RN - 0 (Water Pollutants, Chemical) RN - 0 (Xenobiotics) RN - 138391-32-9 (Aryl Hydrocarbon Receptor Nuclear Translocator) SB - IM MH - Animals MH - Aryl Hydrocarbon Receptor Nuclear Translocator MH - Fishes/genetics MH - Gene Expression Profiling MH - Polychlorinated Dibenzodioxins MH - RNA, Messenger MH - Receptors, Aryl Hydrocarbon MH - Transcriptional Activation MH - Transcriptome/*physiology MH - Water Pollutants, Chemical/*toxicity MH - Xenobiotics/*toxicity PMC - PMC6581032 MID - NIHMS1524225 OTO - NOTNLM OT - Aryl-hydrocarbon receptor OT - CYP3A OT - Comparative transcriptomics OT - Gene family expansion OT - POPs toxicology OT - Pollution adaptation COIS- All authors wrote and revised this paper. The authors declare no competing financial interests. EDAT- 2019/03/21 06:00 MHDA- 2019/04/26 06:00 PMCR- 2020/06/15 CRDT- 2019/03/21 06:00 PHST- 2019/01/27 00:00 [received] PHST- 2019/02/26 00:00 [revised] PHST- 2019/02/27 00:00 [accepted] PHST- 2019/03/21 06:00 [pubmed] PHST- 2019/04/26 06:00 [medline] PHST- 2019/03/21 06:00 [entrez] PHST- 2020/06/15 00:00 [pmc-release] AID - S0048-9697(19)30952-0 [pii] AID - 10.1016/j.scitotenv.2019.02.440 [doi] PST - ppublish SO - Sci Total Environ. 2019 Jun 15;669:621-630. doi: 10.1016/j.scitotenv.2019.02.440. Epub 2019 Feb 28.