PMID- 30893908
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2077-0383 (Print)
IS  - 2077-0383 (Electronic)
IS  - 2077-0383 (Linking)
VI  - 8
IP  - 3
DP  - 2019 Mar 19
TI  - Increased Inflammation and Cardiometabolic Risk in Individuals with Low AMY1 Copy 
      Numbers.
LID - 10.3390/jcm8030382 [doi]
LID - 382
AB  - Lower copy number variations (CNVs) in the salivary amylase gene (AMY1) have been 
      associated with obesity and insulin resistance; however, the relationship between 
      AMY1 and cardiometabolic risk has not been fully elucidated. Using gold-standard 
      measures, we aimed to examine whether AMY1 CNVs are associated with 
      cardiometabolic risk factors in an overweight or obese, otherwise healthy 
      population. Fifty-seven adults (58% male) aged 31.17 +/- 8.44 years with a body 
      mass index (BMI) >/=25 kg/m(2) were included in the study. We measured AMY1 CNVs 
      (qPCR); anthropometry (BMI; body composition by dual-energy X-ray 
      absorptiometry); cardiovascular parameters (blood pressure, serum lipids by 
      ELISA); insulin sensitivity (hyperinsulinaemic(-)euglycaemic clamp), insulin 
      secretion (intravenous glucose tolerance test), and serum inflammation markers 
      (multiplex assays). Based on previous studies and median values, participants 
      were divided into low (</=4) and high (>4) AMY1 CNV groups. Low AMY1 carriers (n = 
      29) had a higher fat mass (40.76 +/- 12.11 versus 33.33 +/- 8.50 kg, p = 0.009) and 
      LDL-cholesterol (3.27 +/- 0.80 versus 2.87 +/- 0.69 mmol/L, p = 0.038), and higher 
      serum levels of interleukin [IL]-6, IL-1beta, tumour necrosis factor-alpha and 
      monocyte chemoattractant protein-1 (MCP-1) (all p < 0.05) compared with high AMY1 
      carriers (n = 28), but there were no differences in glycaemic measures, including 
      insulin sensitivity or secretion (all p > 0.1). Except for MCP-1, the results 
      remained significant in multivariable models adjusted for age, sex, and fat mass 
      (all p < 0.05). Our findings suggest that low AMY1 CNVs are associated with 
      increased cardiovascular disease risk and inflammation, but not glucose 
      metabolism, in overweight or obese adults.
FAU - Marquina, Clara
AU  - Marquina C
AUID- ORCID: 0000-0003-2582-0374
AD  - Monash Centre for Health Research and Implementation, School of Public Health and 
      Preventive Medicine, Monash University, Melbourne 3004, Australia. 
      clara.marquina@monash.edu.
FAU - Mousa, Aya
AU  - Mousa A
AUID- ORCID: 0000-0002-7356-4523
AD  - Monash Centre for Health Research and Implementation, School of Public Health and 
      Preventive Medicine, Monash University, Melbourne 3004, Australia. 
      aya.mousa@monash.edu.
FAU - Belski, Regina
AU  - Belski R
AD  - Faculty of Health, Arts and Design, Swinburne University of Technology, Melbourne 
      3122, Australia. rbelski@swin.edu.au.
FAU - Banaharis, Harry
AU  - Banaharis H
AUID- ORCID: 0000-0003-4445-5376
AD  - Department of Medicine, University of Melbourne, Melbourne 3010, Australia. 
      hbanaharis@me.com.
FAU - Naderpoor, Negar
AU  - Naderpoor N
AD  - Monash Centre for Health Research and Implementation, School of Public Health and 
      Preventive Medicine, Monash University, Melbourne 3004, Australia. 
      negar.naderpoor@monash.edu.
FAU - de Courten, Barbora
AU  - de Courten B
AUID- ORCID: 0000-0001-8760-2511
AD  - Monash Centre for Health Research and Implementation, School of Public Health and 
      Preventive Medicine, Monash University, Melbourne 3004, Australia. 
      barbora.decourten@monash.edu.
LA  - eng
GR  - 1047897/National Health and Medical Research Council/
PT  - Journal Article
DEP - 20190319
PL  - Switzerland
TA  - J Clin Med
JT  - Journal of clinical medicine
JID - 101606588
PMC - PMC6463254
OTO - NOTNLM
OT  - AMY1 copy numbers
OT  - amylase
OT  - cardiometabolic risk
OT  - inflammation
OT  - insulin secretion
OT  - insulin sensitivity
OT  - obesity
OT  - salivary amylase gene
COIS- The authors declare no conflict of interest.
EDAT- 2019/03/22 06:00
MHDA- 2019/03/22 06:01
PMCR- 2019/03/19
CRDT- 2019/03/22 06:00
PHST- 2019/02/12 00:00 [received]
PHST- 2019/03/08 00:00 [revised]
PHST- 2019/03/14 00:00 [accepted]
PHST- 2019/03/22 06:00 [entrez]
PHST- 2019/03/22 06:00 [pubmed]
PHST- 2019/03/22 06:01 [medline]
PHST- 2019/03/19 00:00 [pmc-release]
AID - jcm8030382 [pii]
AID - jcm-08-00382 [pii]
AID - 10.3390/jcm8030382 [doi]
PST - epublish
SO  - J Clin Med. 2019 Mar 19;8(3):382. doi: 10.3390/jcm8030382.