PMID- 30894323
OWN - NLM
STAT- MEDLINE
DCOM- 20200521
LR  - 20200521
IS  - 1873-4596 (Electronic)
IS  - 0891-5849 (Linking)
VI  - 135
DP  - 2019 May 1
TI  - Differential induction of nuclear factor-like 2 signature genes with toll-like 
      receptor stimulation.
PG  - 245-250
LID - S0891-5849(18)32341-4 [pii]
LID - 10.1016/j.freeradbiomed.2019.03.018 [doi]
AB  - Inflammation is associated with production of reactive oxygen species (ROS) and 
      results in the induction of thioredoxin (TXN) and peroxiredoxins (PRDXs) and 
      activation of nuclear factor-like 2 (Nrf2). In this study we have used the mouse 
      RAW 264.7 macrophage and the human THP-1 monocyte cell line to investigate the 
      pattern of expression of three Nrf2 target genes, PRDX1, TXN reductase (TXNRD1) 
      and heme oxygenase (HMOX1), by activation of different Toll-like receptors 
      (TLRs). We found that, while the TLR4 agonist lipopolysaccharide (LPS) induces 
      all three genes, the pattern of induction with agonists for TLR1/2, TLR3, TLR2/6 
      and TLR7/8 differs depending on the gene and the cell line. In all cases, the 
      extent of induction was HMOX1>TXNRD1>PRDX1. Since LPS was a good inducer of all 
      genes in both cell lines, we studied the mechanisms mediating LPS induction of 
      the three genes using mouse RAW 264.7 cells. To assess the role of ROS we used 
      the antioxidant N-acetylcysteine (NAC). Only LPS induction of HMOX1 was inhibited 
      by NAC while that of TXNRD1 and PRDX1 was unaffected. These three genes were also 
      induced by phorbol myristate acetate (PMA), a ROS-inducer acting by activation of 
      protein kinase C (PKC). The protein kinase inhibitor staurosporine inhibited the 
      induction of all three genes by PMA but only that of HMOX1 by LPS. This indicates 
      that activation of these genes by inflammatory agents is regulated by different 
      mechanisms involving either ROS or protein kinases, or both.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Ingram, Sonia
AU  - Ingram S
AD  - Brighton and Sussex Medical School, Falmer, BN1 9PS, United Kingdom.
FAU - Mengozzi, Manuela
AU  - Mengozzi M
AD  - Brighton and Sussex Medical School, Falmer, BN1 9PS, United Kingdom.
FAU - Sacre, Sandra
AU  - Sacre S
AD  - Brighton and Sussex Medical School, Falmer, BN1 9PS, United Kingdom.
FAU - Mullen, Lisa
AU  - Mullen L
AD  - Brighton and Sussex Medical School, Falmer, BN1 9PS, United Kingdom.
FAU - Ghezzi, Pietro
AU  - Ghezzi P
AD  - Brighton and Sussex Medical School, Falmer, BN1 9PS, United Kingdom. Electronic 
      address: p.ghezzi@bsms.ac.uk.
LA  - eng
PT  - Journal Article
DEP - 20190317
PL  - United States
TA  - Free Radic Biol Med
JT  - Free radical biology & medicine
JID - 8709159
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, mouse)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (TLR3 protein, mouse)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Tlr7 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 3)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptor 7)
RN  - EC 1.11.1.15 (Peroxiredoxins)
RN  - EC 1.11.1.15 (Prdx1 protein, mouse)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.14.14.18 (Hmox1 protein, mouse)
RN  - EC 1.8.1.9 (Thioredoxin Reductase 1)
RN  - EC 1.8.1.9 (Txnrd1 protein, mouse)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - H88EPA0A3N (Staurosporine)
SB  - IM
MH  - Animals
MH  - Gene Expression Regulation/drug effects
MH  - Heme Oxygenase-1/genetics
MH  - Inflammation/*genetics/metabolism/pathology
MH  - Lipopolysaccharides/pharmacology
MH  - Membrane Glycoproteins/genetics
MH  - Membrane Proteins/genetics
MH  - Mice
MH  - NF-E2-Related Factor 2/*genetics
MH  - Peroxiredoxins/*genetics
MH  - Protein Kinase C/antagonists & inhibitors/genetics
MH  - RAW 264.7 Cells
MH  - Reactive Oxygen Species/metabolism
MH  - Staurosporine/pharmacology
MH  - Thioredoxin Reductase 1/*genetics
MH  - Toll-Like Receptor 2/genetics
MH  - Toll-Like Receptor 3/genetics
MH  - Toll-Like Receptor 4/genetics
MH  - Toll-Like Receptor 7/genetics
OTO - NOTNLM
OT  - Antioxidant
OT  - Inflammation
OT  - Nrf2
OT  - Peroxiredoxin
OT  - Thioredoxin
OT  - Toll-like receptors
EDAT- 2019/03/22 06:00
MHDA- 2020/05/22 06:00
CRDT- 2019/03/22 06:00
PHST- 2018/10/26 00:00 [received]
PHST- 2019/03/11 00:00 [revised]
PHST- 2019/03/13 00:00 [accepted]
PHST- 2019/03/22 06:00 [pubmed]
PHST- 2020/05/22 06:00 [medline]
PHST- 2019/03/22 06:00 [entrez]
AID - S0891-5849(18)32341-4 [pii]
AID - 10.1016/j.freeradbiomed.2019.03.018 [doi]
PST - ppublish
SO  - Free Radic Biol Med. 2019 May 1;135:245-250. doi: 
      10.1016/j.freeradbiomed.2019.03.018. Epub 2019 Mar 17.