PMID- 30896869
OWN - NLM
STAT- MEDLINE
DCOM- 20190801
LR  - 20211204
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 19
IP  - 5
DP  - 2019 May
TI  - Overexpression of ribonuclease inhibitor induces autophagy in human colorectal 
      cancer cells via the Akt/mTOR/ULK1 pathway.
PG  - 3519-3526
LID - 10.3892/mmr.2019.10030 [doi]
AB  - Ribonuclease inhibitor (RI), also termed angiogenin inhibitor, acts as the 
      inhibitor of ribonucleolytic activity of RNase A and angiogenin. The expression 
      of RI has been investigated in melanoma and bladder cancer cells. However, the 
      precise role of RI in tumorigenesis, in addition to RI‑induced autophagy, remains 
      poorly understood. In the present study, it was demonstrated that RI positively 
      regulated autophagy in human colorectal cancer (CRC) cells as indicated by an 
      increase in light chain 3 (LC3)‑II levels. Furthermore, RI regulated cell 
      survival in HT29 cells. In addition, autophagy‑associated proteins, including 
      beclin‑1 and autophagy‑related protein 13, were increased in response to 
      RI‑induced autophagy, and the protein kinase B (Akt)/mechanistic target of 
      rapamycin (mTOR)/Unc‑51 like autophagy activating kinase (ULK1) pathway may be 
      involved in the activation of autophagy induced by RI overexpression. Taken 
      together, the evidence of the present study indicated that the overexpression of 
      RI induced ATG‑dependent autophagy in CRC cells via the Akt/mTOR/ULK1 pathway, 
      suggesting that the upregulation of RI activity may constitute a novel approach 
      for the treatment of human colorectal carcinoma.
FAU - Tang, Ying
AU  - Tang Y
AD  - Department of Pathology, The Second Affiliated Hospital of Dalian Medical 
      University, Dalian, Liaoning 116023, P.R. China.
FAU - Ren, Feng
AU  - Ren F
AD  - Department of Biochemistry and Molecular Biology, Dalian Medical University, 
      Dalian, Liaoning 116044, P.R. China.
FAU - Cong, Xi
AU  - Cong X
AD  - Department of Biochemistry and Molecular Biology, Dalian Medical University, 
      Dalian, Liaoning 116044, P.R. China.
FAU - Kong, Ying
AU  - Kong Y
AD  - Department of Biochemistry and Molecular Biology, Dalian Medical University, 
      Dalian, Liaoning 116044, P.R. China.
FAU - Tian, Yuxiang
AU  - Tian Y
AD  - Department of Biochemistry and Molecular Biology, Dalian Medical University, 
      Dalian, Liaoning 116044, P.R. China.
FAU - Xu, Yuefei
AU  - Xu Y
AD  - Department of Biochemistry and Molecular Biology, Dalian Medical University, 
      Dalian, Liaoning 116044, P.R. China.
FAU - Fan, Jianhui
AU  - Fan J
AD  - Department of Biochemistry and Molecular Biology, Dalian Medical University, 
      Dalian, Liaoning 116044, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190314
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Placental Hormones)
RN  - 120178-77-0 (placental ribonuclease inhibitor)
RN  - EC 2.7.11.1 (Autophagy-Related Protein-1 Homolog)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (ULK1 protein, human)
MH  - Autophagy/genetics
MH  - Autophagy-Related Protein-1 Homolog/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Survival/genetics
MH  - Colorectal Neoplasms/*genetics/*metabolism/pathology
MH  - *Gene Expression
MH  - HT29 Cells
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/*metabolism
MH  - Placental Hormones/*genetics
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - *Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
PMC - PMC6472130
EDAT- 2019/03/22 06:00
MHDA- 2019/08/02 06:00
PMCR- 2019/03/14
CRDT- 2019/03/22 06:00
PHST- 2018/06/19 00:00 [received]
PHST- 2019/03/06 00:00 [accepted]
PHST- 2019/03/22 06:00 [pubmed]
PHST- 2019/08/02 06:00 [medline]
PHST- 2019/03/22 06:00 [entrez]
PHST- 2019/03/14 00:00 [pmc-release]
AID - mmr-19-05-3519 [pii]
AID - 10.3892/mmr.2019.10030 [doi]
PST - ppublish
SO  - Mol Med Rep. 2019 May;19(5):3519-3526. doi: 10.3892/mmr.2019.10030. Epub 2019 Mar 
      14.