PMID- 30899394
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 11
IP  - 2
DP  - 2019
TI  - Long noncoding RNA HCP5 contributes to epithelial-mesenchymal transition in 
      colorectal cancer through ZEB1 activation and interacting with miR-139-5p.
PG  - 953-963
AB  - Long noncoding RNAs (lncRNAs) play key roles in various malignancy pathogenesis. 
      However, the mechanisms remain poorly understood in the development and 
      progression of colorectal cancer (CRC). Here, we focused on the specific role of 
      human leukocyte antigen (HLA) Complex P5 (HCP5) in CRC. Quantitative real-time 
      PCR (qRT-PCR) analysis and western blot were used to assess the expression of 
      HCP5 in CRC tissues. The association between the expressions of HCP5 and 
      miR-139-5p was assessed by Pearson's correlation analysis. The prognosis of CRC 
      patients was analyzed by Kaplan-Meier survival analysis. Specific siRNAs were 
      stably transfected into CRC cells with lentivirus approaches. The proliferative, 
      migrative and invasive capacities of CRC cells were detected by Transwell, MTT 
      and scratch assay, respectively. Dual-luciferase assay was performed to measure 
      miR-139-5p-targeted relationship with lncRNA HCP5. HCP5 overexpression and of 
      miR-139-5p downregulation were dramatically correlated with low TNM stage, poor 
      differentiation, low tumor depth invasion in CRC patients (P < 0.05). Besides, 
      HCP5 overexpression or ZEB1 knockdown repressed Snail family transcriptional 
      repressor (SNAI) and vimentin expressions, upregulated E-cadherin expression, and 
      inhibited cell proliferation and metastasis (P < 0.05). Moreover, luciferase 
      reporter assay demonstrated that miR-139-5p was a directly target of HCP5 (P < 
      0.05). Overall, the present study indicated that HCP5 played a key regulator in 
      CRC development and progression by targeting HCP5/miR-139-5p/ZEB1 axis, which may 
      serve as a novel therapeutic target for CRC therapy.
FAU - Yang, Cheng
AU  - Yang C
AD  - Department of Hepatobiliary, Henan University of Science and Technology First 
      Affiliated Hospital Luoyang 471000, Henan Province, China.
FAU - Sun, Junjun
AU  - Sun J
AD  - Department of Hepatobiliary, Henan University of Science and Technology First 
      Affiliated Hospital Luoyang 471000, Henan Province, China.
FAU - Liu, Weifeng
AU  - Liu W
AD  - Department of Hepatobiliary, Henan University of Science and Technology First 
      Affiliated Hospital Luoyang 471000, Henan Province, China.
FAU - Yang, Yanhui
AU  - Yang Y
AD  - Department of Hepatobiliary, Henan University of Science and Technology First 
      Affiliated Hospital Luoyang 471000, Henan Province, China.
FAU - Chu, Zhijie
AU  - Chu Z
AD  - Department of Hepatobiliary, Henan University of Science and Technology First 
      Affiliated Hospital Luoyang 471000, Henan Province, China.
FAU - Yang, Tianbao
AU  - Yang T
AD  - Department of Hepatobiliary, Henan University of Science and Technology First 
      Affiliated Hospital Luoyang 471000, Henan Province, China.
FAU - Gui, Yang
AU  - Gui Y
AD  - Department of Hepatobiliary, Henan University of Science and Technology First 
      Affiliated Hospital Luoyang 471000, Henan Province, China.
FAU - Wang, Du
AU  - Wang D
AD  - Department of Hepatobiliary, Henan University of Science and Technology First 
      Affiliated Hospital Luoyang 471000, Henan Province, China.
LA  - eng
PT  - Journal Article
DEP - 20190215
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC6413275
OTO - NOTNLM
OT  - Colorectal cancer (CRC)
OT  - human leukocyte antigen (HLA) complex P5 (HCP5)
OT  - long noncoding RNAs (lncRNA)
OT  - metastasis
OT  - miR-139-5p
OT  - proliferation
COIS- None.
EDAT- 2019/03/23 06:00
MHDA- 2019/03/23 06:01
PMCR- 2019/02/15
CRDT- 2019/03/23 06:00
PHST- 2018/11/19 00:00 [received]
PHST- 2018/12/24 00:00 [accepted]
PHST- 2019/03/23 06:00 [entrez]
PHST- 2019/03/23 06:00 [pubmed]
PHST- 2019/03/23 06:01 [medline]
PHST- 2019/02/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2019 Feb 15;11(2):953-963. eCollection 2019.