PMID- 30902570
OWN - NLM
STAT- MEDLINE
DCOM- 20191011
LR  - 20200611
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 703
DP  - 2019 Jun 11
TI  - Differential regulation of thyrotropin-releasing hormone mRNA expression in the 
      paraventricular nucleus and dorsomedial hypothalamus in OLETF rats.
PG  - 79-85
LID - S0304-3940(19)30186-7 [pii]
LID - 10.1016/j.neulet.2019.03.030 [doi]
AB  - Thyrotropin-releasing hormone (TRH) plays an important role in the regulation of 
      energy balance. While the regulation of TRH in the paraventricular nucleus (PVN) 
      in response to changes of energy balance has been well studied, how TRH is 
      regulated in the dorsomedial hypothalamus (DMH) in maintaining energy homeostasis 
      remains unclear. Here, we assessed the effects of food restriction and exercise 
      on hypothalamic Trh expression using Otsuka Long-Evens Tokushima Fatty (OLETF) 
      rats. Sedentary ad lib fed OLETF rats (OLETF-SED) became hyperphagic and obese. 
      These alterations were prevented in OLETF rats with running wheel access 
      (OLETF-RW) or food restriction in which their food was pair-fed (OLETF-PF) to the 
      intake of lean control rats (LETO-SED). Evaluation of hypothalamic gene 
      expression revealed that Trh mRNA expression was increased in the PVN of 
      OLETF-SED rats and normalized in OLETF-RW and OLETF-PF rats compared to LETO-SED 
      rats. In contrast, the expression of Trh in the DMH was decreased in OLETF-SED 
      rats relative to LETO-SED rats. This alteration was reversed in OLETF-RW rats as 
      seen in LETO-SED rats, but food restriction resulted in a significant increase in 
      DMH Trh expression in OLETF-PF rats compared to LETO-SED rats. Strikingly, while 
      Trh mRNA expression was decreased in the PVN of intact rats in response to acute 
      food deprivation, food deprivation resulted in increased expression of Trh in the 
      DMH. Together, these results demonstrate the differential regulation of Trh 
      expression in the PVN and DMH in OLETF rats and suggest that DMH TRH also 
      contributes to hypothalamic regulation of energy balance.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Zhang, Ni
AU  - Zhang N
AD  - Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School 
      of Medicine, Baltimore, MD, USA; Department of Psychiatry, West China Hospital, 
      Sichuan University, Chengdu, Sichuan, China.
FAU - Zhang, Hai-Ying
AU  - Zhang HY
AD  - Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School 
      of Medicine, Baltimore, MD, USA.
FAU - Bi, Sophia A
AU  - Bi SA
AD  - Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School 
      of Medicine, Baltimore, MD, USA.
FAU - Moran, Timothy H
AU  - Moran TH
AD  - Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School 
      of Medicine, Baltimore, MD, USA.
FAU - Bi, Sheng
AU  - Bi S
AD  - Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School 
      of Medicine, Baltimore, MD, USA. Electronic address: sbi@jhmi.edu.
LA  - eng
GR  - R01 DK103710/DK/NIDDK NIH HHS/United States
GR  - R01 DK104867/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20190319
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Blood Glucose)
RN  - 0 (Leptin)
RN  - 0 (RNA, Messenger)
RN  - 5Y5F15120W (Thyrotropin-Releasing Hormone)
SB  - IM
MH  - Animals
MH  - Blood Glucose
MH  - Body Weight
MH  - Diet, Reducing
MH  - Dorsomedial Hypothalamic Nucleus/*metabolism
MH  - Eating
MH  - Leptin/blood
MH  - Male
MH  - Obesity/physiopathology/psychology
MH  - Paraventricular Hypothalamic Nucleus/*metabolism
MH  - Physical Conditioning, Animal
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Thyrotropin-Releasing Hormone/genetics/*metabolism
PMC - PMC6604803
MID - NIHMS1525719
OTO - NOTNLM
OT  - Dorsomedial hypothalamic nucleus
OT  - Exercise
OT  - Food restriction
OT  - Neuropeptide Y
OT  - Obesity
OT  - Thyrotropin-releasing hormone
COIS- Declarations of interest All authors declare no conflict of interest related to 
      this manuscript.
EDAT- 2019/03/25 06:00
MHDA- 2019/10/12 06:00
PMCR- 2020/06/11
CRDT- 2019/03/24 06:00
PHST- 2019/01/04 00:00 [received]
PHST- 2019/03/17 00:00 [revised]
PHST- 2019/03/18 00:00 [accepted]
PHST- 2019/03/25 06:00 [pubmed]
PHST- 2019/10/12 06:00 [medline]
PHST- 2019/03/24 06:00 [entrez]
PHST- 2020/06/11 00:00 [pmc-release]
AID - S0304-3940(19)30186-7 [pii]
AID - 10.1016/j.neulet.2019.03.030 [doi]
PST - ppublish
SO  - Neurosci Lett. 2019 Jun 11;703:79-85. doi: 10.1016/j.neulet.2019.03.030. Epub 
      2019 Mar 19.