PMID- 30903388
OWN - NLM
STAT- MEDLINE
DCOM- 20191223
LR  - 20200309
IS  - 1436-5073 (Electronic)
IS  - 0026-3672 (Linking)
VI  - 186
IP  - 4
DP  - 2019 Mar 22
TI  - An integrated magnetic microfluidic chip for rapid immunodetection of the 
      prostate specific antigen using immunomagnetic beads.
PG  - 252
LID - 10.1007/s00604-019-3349-1 [doi]
AB  - The authors describe an integrated microfluidic chip for immunodetection of the 
      prostate specific antigen (PSA) by using giant magnetoimpedance (GMI) sensor. 
      This chip contains an immunoreaction platform and a biomarker detection system. 
      The immunoreaction platform contains an incubation chamber and a reactive chamber 
      to implement immunological reaction in microfluidics. The system can detect PSA 
      rapidly with ultra-high sensitivity. Both are fabricated by MEMS technology. 
      Immunomagnetic beads (If PSA binds to its antibody (that is labeled with 
      immunomagnetic beads; IMBs) it will be trapped on the surface of self-assembled 
      film. Trapped IMBs generate a stray magnetic field under the magnetization of the 
      external applied magnetic field and can be detected by the GMI sensor. The chip 
      can detect PSA with a detection limit as low as 0.1 ng ∙ mL(-1) and works in the 
      0.1 ng ∙ mL(-1) to 20 ng ∙ mL(-1 )concentration range. Compared to established 
      GMI biosensors, the magnetic microfluidic chip reduces assay time, and lends 
      itself to fast detection. It also avoids complex handling steps, enhances 
      reaction efficiency and decreases experimental errors. Graphical abstract An 
      integrated magnetic microfluidic chip which contains immunoreaction platform and 
      biomarker detection system was designed and microfabricated by 
      micro-electromechanical systems (MEMS) technology to detect prostate specific 
      antigen (PSA) rapidly, and has promise in Point-of-care (PoC) diagnostic 
      applications.
FAU - Feng, Zhu
AU  - Feng Z
AUID- ORCID: 0000-0002-4305-1700
AD  - Key Laboratory of Thin Film and Microfabrication (Ministry of Education), 
      Department of Micro-Nano Electronics, School of Electronic Information and 
      Electrical Engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 
      Shanghai, 200240, China. feng.z@sjtu.edu.cn.
FAU - Zhi, Shaotao
AU  - Zhi S
AD  - Key Laboratory of Thin Film and Microfabrication (Ministry of Education), 
      Department of Micro-Nano Electronics, School of Electronic Information and 
      Electrical Engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 
      Shanghai, 200240, China.
FAU - Guo, Lei
AU  - Guo L
AD  - Key Laboratory of Thin Film and Microfabrication (Ministry of Education), 
      Department of Micro-Nano Electronics, School of Electronic Information and 
      Electrical Engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 
      Shanghai, 200240, China.
FAU - Zhou, Yong
AU  - Zhou Y
AD  - Key Laboratory of Thin Film and Microfabrication (Ministry of Education), 
      Department of Micro-Nano Electronics, School of Electronic Information and 
      Electrical Engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 
      Shanghai, 200240, China.
FAU - Lei, Chong
AU  - Lei C
AD  - Key Laboratory of Thin Film and Microfabrication (Ministry of Education), 
      Department of Micro-Nano Electronics, School of Electronic Information and 
      Electrical Engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 
      Shanghai, 200240, China. leiqhd@sjtu.edu.cn.
LA  - eng
GR  - 61273065/National Natural Science Foundation of China/International
GR  - 2012BAK08B05/National Science and Technology Support Program/International
GR  - AgriX2015005/Support fund of Shanghai Jiao Tong University/International
GR  - USCAST2015-2/Support fund of Joint research center for advanced aerospace 
      technology of Shanghai Academy of Spaceflight Technology-Shanghai Jiao Tong 
      University/International
GR  - 15GFZ-JJ02-05/Support fund of aerospace technology/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190322
PL  - Austria
TA  - Mikrochim Acta
JT  - Mikrochimica acta
JID - 7808782
RN  - 0 (Antibodies)
RN  - 0 (Biomarkers)
RN  - EC 3.4.21.77 (Prostate-Specific Antigen)
SB  - IM
MH  - Animals
MH  - Antibodies/immunology
MH  - Biomarkers/analysis
MH  - Equipment Design
MH  - Humans
MH  - Immunoassay/*instrumentation/methods
MH  - Immunomagnetic Separation/*instrumentation/methods
MH  - *Lab-On-A-Chip Devices
MH  - Limit of Detection
MH  - Mice
MH  - Microfluidic Analytical Techniques/*instrumentation/methods
MH  - Prostate-Specific Antigen/*analysis/immunology
OTO - NOTNLM
OT  - Biomarker detection
OT  - Biosensor
OT  - Giant magnetoimpedance
OT  - Immunomagnetic beads
OT  - Immunoreaction platform
OT  - Magnetic sensor
OT  - Micro-electromechanical systems
OT  - Microfluidic chip
OT  - Point-of-care diagnostics
OT  - Prostate specific antigen
EDAT- 2019/03/25 06:00
MHDA- 2019/12/24 06:00
CRDT- 2019/03/24 06:00
PHST- 2019/01/23 00:00 [received]
PHST- 2019/02/27 00:00 [accepted]
PHST- 2019/03/24 06:00 [entrez]
PHST- 2019/03/25 06:00 [pubmed]
PHST- 2019/12/24 06:00 [medline]
AID - 10.1007/s00604-019-3349-1 [pii]
AID - 10.1007/s00604-019-3349-1 [doi]
PST - epublish
SO  - Mikrochim Acta. 2019 Mar 22;186(4):252. doi: 10.1007/s00604-019-3349-1.