PMID- 30904528
OWN - NLM
STAT- MEDLINE
DCOM- 20190909
LR  - 20190909
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 131
DP  - 2019 Jun 15
TI  - Dissecting the effect of polyethylene glycol on the enzymatic hydrolysis of 
      diverse lignocellulose.
PG  - 676-681
LID - S0141-8130(19)30499-4 [pii]
LID - 10.1016/j.ijbiomac.2019.03.131 [doi]
AB  - Natural lignocellulose is used as raw material to produce chemicals through 
      biological transformation. The accessibility of cellulase to substrate was also 
      one of the limiting factors of industrial production. Polyethylene glycol (PEG) 
      can be used as additive in enzymatic hydrolysis of lignocellulose. In this study, 
      enzymatic activity on simultaneous or non-simultaneous addition of PEG 4000 was 
      investigated, and the partly delignified rice straw, the rice straw and filter 
      paper were used as substrates, respectively. Enzyme activity was characterized by 
      reducing sugar concentration in supernatant which was quantified through 
      3,5-dinitrosalicylic acid (DNS) method. Addition of PEG has been proven to 
      facilitate enzymatic hydrolysis of lignocellulosic materials. Furthermore, PEG 
      had the positive effect on hydrolytic enzyme activity of pure cellulose materials 
      without lignin. Changes in lignocellulose materials have been observed by 
      inverted microscope and Scanning electron microscope (SEM), and no chemical 
      changes were shown by Fourier transform infrared spectroscopy (FTIR). The 
      promotion of PEG on enzymatic hydrolysis of pure cellulose materials may be due 
      to its loose physical structure and similar phenomenon in natural lignin 
      materials. PEG loosens the physical structure of lignocellulose, thus 
      facilitating enzymatic hydrolysis. This may be a new idea to optimize the 
      lignocellulosic enzymatic hydrolysis process.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Li, Huanan
AU  - Li H
AD  - State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life 
      Sciences, Hubei University, Wuhan 430062, PR China; Hubei Key Laboratory of 
      Industrial Biotechnology, School of Life Science, Hubei University, Wuhan 430062, 
      PR China.
FAU - Wang, Chaoying
AU  - Wang C
AD  - State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life 
      Sciences, Hubei University, Wuhan 430062, PR China; Hubei Key Laboratory of 
      Industrial Biotechnology, School of Life Science, Hubei University, Wuhan 430062, 
      PR China.
FAU - Xiao, Wenjing
AU  - Xiao W
AD  - State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life 
      Sciences, Hubei University, Wuhan 430062, PR China.
FAU - Yang, Yuxian
AU  - Yang Y
AD  - State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life 
      Sciences, Hubei University, Wuhan 430062, PR China.
FAU - Hu, Pan
AU  - Hu P
AD  - State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life 
      Sciences, Hubei University, Wuhan 430062, PR China.
FAU - Dai, Yujun
AU  - Dai Y
AD  - Hubei Province Research Center of Engineering Technology for Utilization of 
      Botanical Functional Ingredients, Hubei Engineering University, Xiaogan 432000, 
      PR China.
FAU - Jiang, Zhengbing
AU  - Jiang Z
AD  - State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life 
      Sciences, Hubei University, Wuhan 430062, PR China; Hubei Key Laboratory of 
      Industrial Biotechnology, School of Life Science, Hubei University, Wuhan 430062, 
      PR China. Electronic address: zhbjiang@hubu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190320
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Carbohydrates)
RN  - 11132-73-3 (lignocellulose)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9005-53-2 (Lignin)
RN  - EC 3.2.1.4 (Cellulase)
SB  - IM
MH  - Carbohydrates/chemistry
MH  - Cellulase/*chemistry
MH  - Hydrolysis
MH  - Lignin/*chemistry
MH  - Molecular Structure
MH  - Polyethylene Glycols/*chemistry
MH  - Substrate Specificity
OTO - NOTNLM
OT  - Cellulase
OT  - Lignocellulose
OT  - PEG 4000
EDAT- 2019/03/25 06:00
MHDA- 2019/09/10 06:00
CRDT- 2019/03/25 06:00
PHST- 2019/01/22 00:00 [received]
PHST- 2019/03/06 00:00 [revised]
PHST- 2019/03/19 00:00 [accepted]
PHST- 2019/03/25 06:00 [pubmed]
PHST- 2019/09/10 06:00 [medline]
PHST- 2019/03/25 06:00 [entrez]
AID - S0141-8130(19)30499-4 [pii]
AID - 10.1016/j.ijbiomac.2019.03.131 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2019 Jun 15;131:676-681. doi: 
      10.1016/j.ijbiomac.2019.03.131. Epub 2019 Mar 20.