PMID- 3090524
OWN - NLM
STAT- MEDLINE
DCOM- 19860916
LR  - 20191029
IS  - 0344-0338 (Print)
IS  - 0344-0338 (Linking)
VI  - 181
IP  - 2
DP  - 1986 May
TI  - Effects of methylnitrosourea on visualization of acridine orange binding to DNA 
      in mouse lymphoma L-1210 cells.
PG  - 206-12
AB  - The purpose of this study is to examine effects of methylnitrosourea (MNU) on 
      electron microscopic visualization of acridine orange (AO) binding to DNA in 
      mouse lymphoma L-1210 cells and to demonstrate alteration of the 
      euchromatin/heterochromatin ratio by morphometry. [3H] uridine uptake into RNA 
      molecule is inhibited and percentage of AO positive cells is decreased to 
      approximately 20% of that of the untreated control cells by treatment of the 
      lymphoma cells with MNU. When the MNU-treated cells are cultured in the presence 
      of 3-amino-benzamide, a specific inhibitor of poly(ADP-ribose) synthetase, the 
      depression in RNA synthesis is prevented, and percentage of AO positive cells as 
      well as numbers of AO chromatin interaction products per single cell nucleus are 
      also completely recovered as compared to those in the untreated cells. In the 
      MNU-treated cells the decreased number of AO positive cells coincides with a 
      reduced [3H] uridine uptake as well as with a lowered euchromatin/heterochromatin 
      ratio. The results suggest that visualization of AO chromatin interaction 
      products in the proliferating cells is related not only to RNA synthesis in the 
      cells, but also to the euchromatin/heterochromatin ratio.
FAU - Moriki, T
AU  - Moriki T
FAU - Hiroi, M
AU  - Hiroi M
FAU - Hara, H
AU  - Hara H
FAU - Taniguchi, T
AU  - Taniguchi T
FAU - Shizuta, Y
AU  - Shizuta Y
FAU - Yamane, T
AU  - Yamane T
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 0 (Benzamides)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Poly(ADP-ribose) Polymerase Inhibitors)
RN  - 684-93-5 (Methylnitrosourea)
RN  - 8J365YF1YH (3-aminobenzamide)
RN  - F30N4O6XVV (Acridine Orange)
RN  - WHI7HQ7H85 (Uridine)
SB  - IM
MH  - *Acridine Orange
MH  - Animals
MH  - Benzamides/pharmacology
MH  - DNA, Neoplasm/*analysis
MH  - Leukemia L1210/*metabolism
MH  - Methylnitrosourea/*pharmacology
MH  - Mice
MH  - Microscopy, Electron
MH  - Poly(ADP-ribose) Polymerase Inhibitors
MH  - Transcription, Genetic/drug effects
MH  - Uridine/metabolism
EDAT- 1986/05/01 00:00
MHDA- 1986/05/01 00:01
CRDT- 1986/05/01 00:00
PHST- 1986/05/01 00:00 [pubmed]
PHST- 1986/05/01 00:01 [medline]
PHST- 1986/05/01 00:00 [entrez]
AID - 10.1016/s0344-0338(86)80012-7 [doi]
PST - ppublish
SO  - Pathol Res Pract. 1986 May;181(2):206-12. doi: 10.1016/s0344-0338(86)80012-7.