PMID- 30905893
OWN - NLM
STAT- MEDLINE
DCOM- 20190802
LR  - 20190802
IS  - 1347-3700 (Electronic)
IS  - 0386-7196 (Linking)
VI  - 44
IP  - 1
DP  - 2019
TI  - Long Noncoding RNA LINC00202 Promotes Tumor Progression by Sponging miR-3619-5p 
      in Retinoblastoma.
PG  - 51-60
LID - 10.1247/csf.18033 [doi]
AB  - Retinoblastoma (RB) is the most common intraocular malignancy in childhood, and 
      the prognosis in the advanced RB is poor. It is urgent to find novel therapeutic 
      targets. Long noncoding RNAs (lncRNAs) have critical functions in cancer 
      progression, and lncRNA LINC00202 is found associated with poor prognosis in RB. 
      However, the functions of LINC00202 in RB remain unclear. We employed qRT-PCR and 
      immunoblot to detect the expression levels of mRNAs and proteins, respectively. 
      Cell proliferation was determined by CCK-8 assay and colony formation assay. 
      Transwell assays were applied to evaluate the cell abilities of migration and 
      invasion. Luciferase reporter assay was applied to examine RNA stability, and RNA 
      pulldown assays were used to detect interaction between lncRNA and microRNA 
      (miRNA). LINC00202 expression in RB tissues is higher than that in the paired 
      adjacent normal tissues, which has correlation with poor prognosis in RB. RB cell 
      proliferation, migration and invasion were weakened by LINC00202 depletion, but 
      enhanced by LINC00202 overexpression. MiR-3619-5p was identified to directly bind 
      and mediate LINC00202-promoted RB progression, meanwhile, miR-3619-5p directly 
      regulated expression of an oncongene, RIN1. Moreover, RIN1 knockdown completely 
      blocked miR-3619-5p-enhanced RB progression. In summary, high LINC00202 levels 
      are correlated with poor prognosis in RB, and it promotes RB progression by 
      sponging miR-3619-5p and therefore up-regulating RIN1 expression.Key words: 
      LINC00202, miR-3619-5p, retinoblastoma, progression, RIN1.
FAU - Yan, Guigang
AU  - Yan G
AD  - Department of Ophthalmology, the Affiliated Yantai Yuhuangding Hospital of 
      Qingdao University.
FAU - Su, Yi
AU  - Su Y
AD  - Department of Radiation Oncology, the Affiliated Yantai Yuhuangding Hospital of 
      Qingdao University.
FAU - Ma, Zhao
AU  - Ma Z
AD  - Department of Radiation Oncology, the Affiliated Yantai Yuhuangding Hospital of 
      Qingdao University.
FAU - Yu, Lianzhi
AU  - Yu L
AD  - Department of Physical Examination, the Affiliated Yantai Yuhuangding Hospital of 
      Qingdao University.
FAU - Chen, Ning
AU  - Chen N
AD  - Department of Ophthalmology, the Affiliated Yantai Yuhuangding Hospital of 
      Qingdao University.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Cell Struct Funct
JT  - Cell structure and function
JID - 7608465
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (MIRN-3619 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RIN1 protein, human)
RN  - 0 (RNA, Long Noncoding)
MH  - Base Sequence
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - *Disease Progression
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/genetics
MH  - MicroRNAs/*genetics
MH  - Neoplasm Invasiveness/genetics
MH  - Prognosis
MH  - RNA, Long Noncoding/*genetics
MH  - Retinal Neoplasms/diagnosis/*genetics/*pathology
MH  - Retinoblastoma/diagnosis/*genetics/*pathology
MH  - Up-Regulation/genetics
OTO - NOTNLM
OT  - LINC00202
OT  - RIN1
OT  - miR-3619-5p
OT  - progression
OT  - retinoblastoma
EDAT- 2019/03/25 06:00
MHDA- 2019/08/03 06:00
CRDT- 2019/03/26 06:00
PHST- 2019/03/26 06:00 [entrez]
PHST- 2019/03/25 06:00 [pubmed]
PHST- 2019/08/03 06:00 [medline]
AID - 10.1247/csf.18033 [doi]
PST - ppublish
SO  - Cell Struct Funct. 2019;44(1):51-60. doi: 10.1247/csf.18033.