PMID- 30906464
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 17
IP  - 4
DP  - 2019 Apr
TI  - Correlation of cardiomyocyte apoptosis with duration of hypertension, severity of 
      hypertension and caspase-3 expression in hypertensive rats.
PG  - 2741-2745
LID - 10.3892/etm.2019.7249 [doi]
AB  - Correlation of cardiomyocyte apoptosis with duration of hypertension, severity of 
      hypertension and caspase-3 expression in hypertensive rats was analyzed. Sixty 
      male Sprague-Dawley (SD) rats were selected and randomly divided into the 
      observation group (n=30) and control group (n=30), and the rat models of 
      hypertension were established by virtue of transverse aortic constriction (TAC). 
      The rats in the two groups were further divided into the 7-day subgroup (n=10), 
      14-day subgroup (n=10) and 28-day subgroup (n=10), respectively according to 
      their survival time after TAC. The blood pressure values of the rats in each 
      group were measured through intubation of carotid artery to calculate the mean 
      arterial pressure (MAP). The conditions of cardiomyocyte apoptosis were detected 
      using terminal dexynucleotidyl transferase-mediated dUTP nick end labeling 
      (TUNEL) assay. Enzyme-linked immunosorbent assay (ELISA) was applied to measure 
      the expression of caspase-3 in the myocardial tissues, and correlation analysis 
      was performed. The MAPs in 7-, 14- and 28-day subgroups of the observation group 
      were significantly higher than those in the corresponding subgroups of the 
      control group (P<0.05). The 7-, 14- and 28-day subgroups of the observation group 
      had remarkably elevated myocardial caspase-3 expression levels compared with the 
      subgroups of the control group (P<0.05). The apoptosis rates of myocardial cells 
      in the three subgroups of the observation group were obviously higher than those 
      in the corresponding subgroups of the control group (P<0.05). Pearson's 
      correlation analysis indicated that the cardiomyocyte apoptosis rate of 
      hypertensive rats was positively correlated with the duration of hypertension, 
      severity of hypertension and caspase-3 expression (P<0.05). Hypertension can 
      induce apoptosis of myocardial cells, and the apoptosis becomes more serious with 
      the constantly elevated level and prolonged duration of hypertension. In 
      addition, the activity of caspase-3 has a close correlation with cardiomyocyte 
      apoptosis.
FAU - Wang, Qinglei
AU  - Wang Q
AD  - Intracardiac Intensive Care Unit, Affiliated Hospital of Jining Medical 
      University, Jining, Shandong 272000, P.R. China.
FAU - Cui, Yinghua
AU  - Cui Y
AD  - Intracardiac Intensive Care Unit, Affiliated Hospital of Jining Medical 
      University, Jining, Shandong 272000, P.R. China.
FAU - Lin, Nan
AU  - Lin N
AD  - Department of Cardiology (I), Affiliated Hospital of Jining Medical University, 
      Jining, Shandong 272000, P.R. China.
FAU - Pang, Shuchao
AU  - Pang S
AD  - Central Laboratory, Affiliated Hospital of Jining Medical University, Jining, 
      Shandong 272000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190207
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6425263
OTO - NOTNLM
OT  - apoptosis
OT  - caspase-3
OT  - hypertension
OT  - myocardial cells
EDAT- 2019/03/25 06:00
MHDA- 2019/03/25 06:01
PMCR- 2019/02/07
CRDT- 2019/03/26 06:00
PHST- 2018/03/30 00:00 [received]
PHST- 2019/02/01 00:00 [accepted]
PHST- 2019/03/26 06:00 [entrez]
PHST- 2019/03/25 06:00 [pubmed]
PHST- 2019/03/25 06:01 [medline]
PHST- 2019/02/07 00:00 [pmc-release]
AID - ETM-0-0-7249 [pii]
AID - 10.3892/etm.2019.7249 [doi]
PST - ppublish
SO  - Exp Ther Med. 2019 Apr;17(4):2741-2745. doi: 10.3892/etm.2019.7249. Epub 2019 Feb 
      7.