PMID- 30908739
OWN - NLM
STAT- MEDLINE
DCOM- 20190613
LR  - 20220408
IS  - 1365-3164 (Electronic)
IS  - 0959-4493 (Linking)
VI  - 30
IP  - 3
DP  - 2019 Jun
TI  - Agreement of serum allergen test results with unblocked and blocked IgE against 
      cross-reactive carbohydrate determinants (CCD) and intradermal test results in 
      atopic dogs.
PG  - 195-e61
LID - 10.1111/vde.12742 [doi]
AB  - BACKGROUND: Tests for allergen-specific immunoglobulin E (IgE) are used to select 
      allergens for immunotherapy in atopic dogs. Antibodies against cross-reactive 
      carbohydrate determinants (anti-CCD IgE) have been identified in serum samples of 
      atopic dogs. Their presence in humans is a known cause of clinically irrelevant 
      polysensitization to plant allergens. OBJECTIVES: To compare the results of an 
      intradermal test (IDT) and a serum test for allergen-specific IgE, with and 
      without blocking anti-CCD IgE, before testing in dogs. ANIMALS: Thirty-one 
      privately owned dogs with atopic dermatitis. METHODS AND MATERIALS: Dogs were 
      prospectively skin tested and their serum samples were analysed for anti-CCD IgE. 
      An Fc-epsilon receptor-based serum test for allergen-specific IgE was performed with 
      and without blocking anti-CCD IgE. RESULTS: In dogs with negative anti-CCD IgE 
      samples, the agreement between the results of the serum test and the IDT was 
      substantial (kappa = 0.71). Dogs with positive anti-CCD IgE samples (38.7%) showed no 
      agreement between serum and skin testing (kappa = -0.35), blocking anti-CCD IgE in 
      those samples resulted in a fair agreement (kappa = 0.43). Anti-CCD IgE positive sera 
      had multiple positive results for grass and weed allergens, and blocking 
      decreased them markedly. CONCLUSION AND CLINICAL IMPORTANCE: Intradermal testing 
      agreed best with serum testing in dogs with no detectable anti-CCD IgE. Sera 
      containing anti-CCD IgE had no agreement with IDT. Test agreement was improved by 
      blocking the anti-CCD IgE. Apparent serum test polysensitization to plant 
      allergens was associated with anti-CCD IgE.
CI  - (c) 2019 ESVD and ACVD.
FAU - Gedon, Natalie K Y
AU  - Gedon NKY
AUID- ORCID: 0000-0003-0224-9144
AD  - Centre for Clinical Veterinary Medicine, LMU Munich, Veterinaerstrasse 13, 80539, 
      Muenchen, Germany.
FAU - Boehm, Teresa M S A
AU  - Boehm TMSA
AUID- ORCID: 0000-0001-7886-0017
AD  - Centre for Clinical Veterinary Medicine, LMU Munich, Veterinaerstrasse 13, 80539, 
      Muenchen, Germany.
FAU - Klinger, Christoph J
AU  - Klinger CJ
AUID- ORCID: 0000-0003-1570-3078
AD  - Centre for Clinical Veterinary Medicine, LMU Munich, Veterinaerstrasse 13, 80539, 
      Muenchen, Germany.
FAU - Udraite, Laura
AU  - Udraite L
AD  - Centre for Clinical Veterinary Medicine, LMU Munich, Veterinaerstrasse 13, 80539, 
      Muenchen, Germany.
FAU - Mueller, Ralf S
AU  - Mueller RS
AUID- ORCID: 0000-0001-5835-5910
AD  - Centre for Clinical Veterinary Medicine, LMU Munich, Veterinaerstrasse 13, 80539, 
      Muenchen, Germany.
LA  - eng
GR  - Heska AG; Fribourg, Switzerland/
PT  - Comparative Study
PT  - Journal Article
DEP - 20190324
PL  - England
TA  - Vet Dermatol
JT  - Veterinary dermatology
JID - 9426187
RN  - 0 (Allergens)
RN  - 0 (Carbohydrates)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*immunology
MH  - Animals
MH  - Carbohydrates/*immunology
MH  - Cross Reactions
MH  - Dermatitis, Atopic/diagnosis/*veterinary
MH  - Dog Diseases/diagnosis/immunology
MH  - Dogs
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Immunoglobulin E/*blood
MH  - Intradermal Tests/methods/*veterinary
MH  - Prospective Studies
MH  - Serologic Tests/methods/veterinary
MH  - Skin Tests/*veterinary
EDAT- 2019/03/26 06:00
MHDA- 2019/06/14 06:00
CRDT- 2019/03/26 06:00
PHST- 2019/01/22 00:00 [accepted]
PHST- 2019/03/26 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
PHST- 2019/03/26 06:00 [entrez]
AID - 10.1111/vde.12742 [doi]
PST - ppublish
SO  - Vet Dermatol. 2019 Jun;30(3):195-e61. doi: 10.1111/vde.12742. Epub 2019 Mar 24.