PMID- 30909557
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 8
IP  - 3
DP  - 2019 Mar 23
TI  - The Strong Antioxidant Sheep/Goat Whey Protein Protects Against mTOR 
      Overactivation in Rats: A Mode of Action Mimicking Fasting.
LID - 10.3390/antiox8030071 [doi]
LID - 71
AB  - Whey protein, a by-product of the cheese industry, can be putatively used as a 
      functional food due to its beneficial health properties. The main objective of 
      the present study was to assess in vivo the effect of a sheep/goat whey protein 
      on the plasma amino acid profile and mammalian target of rapamycin (mTOR), a 
      regulator of skeletal myogenesis. A control group was fed with a standard 
      commercial diet while the experimental group received a standard commercial diet 
      plus sheep/goat whey protein for 28 days. Liquid chromatography-tandem mass 
      spectrometry (LC-MS/MS) was conducted to determine plasma amino acid levels while 
      the expression of p70-S6 Kinase 1 (p70-S6K1) in liver and quadriceps muscles was 
      quantified and used as a biomarker of mTOR activity. The results obtained showed 
      a decrease in the levels of essential and branched-chain amino acids (BCAAs) in 
      the experimental group. Furthermore, p70-S6K1 expression was decreased in the 
      liver of rats consumed whey protein. In conclusion, the reduction of amino acid 
      levels and the concomitant inactivation of mTOR imply that whey could potentially 
      act protectively against disorders induced by mTOR overactivation. Intriguingly, 
      this mode of action mimics fasting, an approach with established advantageous 
      health effects.
FAU - Kerasioti, Efthalia
AU  - Kerasioti E
AD  - Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, 
      Mezourlo, 41500 Larissa, Greece. e-f-thalia@hotmail.com.
FAU - Veskoukis, Aristidis
AU  - Veskoukis A
AD  - Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, 
      Mezourlo, 41500 Larissa, Greece. veskoukis@uth.gr.
FAU - Virgiliou, Christina
AU  - Virgiliou C
AD  - Laboratory of Analytical Chemistry, School of Chemistry, Aristotle University of 
      Thessaloniki, 54124 Thessaloniki, Greece. cr_virgi@hotmail.com.
AD  - Biomic_AUTh, Center for Interdisciplinary Research and Innovation (CIRI-AUTH), 
      Balkan Center, 57001 Thessaloniki, Greece. cr_virgi@hotmail.com.
FAU - Theodoridis, Georgios
AU  - Theodoridis G
AUID- ORCID: 0000-0002-2015-108X
AD  - Laboratory of Analytical Chemistry, School of Chemistry, Aristotle University of 
      Thessaloniki, 54124 Thessaloniki, Greece. gtheodor@chem.auth.gr.
AD  - Biomic_AUTh, Center for Interdisciplinary Research and Innovation (CIRI-AUTH), 
      Balkan Center, 57001 Thessaloniki, Greece. gtheodor@chem.auth.gr.
FAU - Taitzoglou, Ioannis
AU  - Taitzoglou I
AD  - School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 
      Thessaloniki, Greece. jotai@vet.auth.gr.
FAU - Kouretas, Dimitrios
AU  - Kouretas D
AD  - Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, 
      Mezourlo, 41500 Larissa, Greece. dkouret@uth.gr.
LA  - eng
GR  - 2016-050-0503-7344/State Scholaship Foundation/
PT  - Journal Article
DEP - 20190323
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC6466539
OTO - NOTNLM
OT  - amino acids
OT  - disease
OT  - fasting
OT  - mTOR
OT  - sheep/goat whey protein
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2019/03/27 06:00
MHDA- 2019/03/27 06:01
PMCR- 2019/03/23
CRDT- 2019/03/27 06:00
PHST- 2019/02/18 00:00 [received]
PHST- 2019/03/21 00:00 [revised]
PHST- 2019/03/21 00:00 [accepted]
PHST- 2019/03/27 06:00 [entrez]
PHST- 2019/03/27 06:00 [pubmed]
PHST- 2019/03/27 06:01 [medline]
PHST- 2019/03/23 00:00 [pmc-release]
AID - antiox8030071 [pii]
AID - antioxidants-08-00071 [pii]
AID - 10.3390/antiox8030071 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2019 Mar 23;8(3):71. doi: 10.3390/antiox8030071.