PMID- 30909643
OWN - NLM
STAT- MEDLINE
DCOM- 20190717
LR  - 20200225
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 20
IP  - 6
DP  - 2019 Mar 24
TI  - Increased Brain-Derived Neurotrophic Factor in Lumbar Dorsal Root Ganglia 
      Contributes to the Enhanced Exercise Pressor Reflex in Heart Failure.
LID - 10.3390/ijms20061480 [doi]
LID - 1480
AB  - An exaggerated exercise pressor reflex (EPR) is associated with excessive 
      sympatho-excitation and exercise intolerance in the chronic heart failure (CHF) 
      state. We hypothesized that brain-derived neurotrophic factor (BDNF) causes the 
      exaggerated EPR via sensitizing muscle mechanosensitive afferents in CHF. 
      Increased BDNF expression was observed in lumbar dorsal root ganglia (DRGs) from 
      CHF rats compared to sham rats. Immunofluorescence data showed a greater increase 
      in the number of BDNF-positive neurons in medium and large-sized DRG 
      subpopulations from CHF rats. Patch clamp data showed that incubation with BDNF 
      for 4(-)6 h, significantly decreased the current threshold-inducing action 
      potential (AP), threshold potential and the number of APs during current 
      injection in Dil-labeled isolectin B4 (IB4)-negative medium-sized DRG neurons 
      (mainly mechano-sensitive) from sham rats. Compared to sham rats, CHF rats 
      exhibited an increased number of APs during current injection in the same DRG 
      subpopulation, which was significantly attenuated by 4-h incubation with 
      anti-BDNF. Finally, chronic epidural delivery of anti-BDNF attenuated the 
      exaggerated pressor response to either static contraction or passive stretch in 
      CHF rats whereas this intervention had no effect on the pressor response to 
      hindlimb arterial injection of capsaicin. These data suggest that increased BDNF 
      in lumbar DRGs contributes to the exaggerated EPR in CHF.
FAU - Schiller, Alicia M
AU  - Schiller AM
AD  - Department of Anesthesiology, University of Nebraska Medical Center, Omaha, NE 
      68198-5850, USA. alicia.schiller@unmc.edu.
FAU - Hong, Juan
AU  - Hong J
AD  - Department of Anesthesiology, University of Nebraska Medical Center, Omaha, NE 
      68198-5850, USA. juan.hong@unmc.edu.
FAU - Xia, Zhiqiu
AU  - Xia Z
AD  - Department of Anesthesiology, University of Nebraska Medical Center, Omaha, NE 
      68198-5850, USA. Zhiqiu.xia@unmc.edu.
FAU - Wang, Han-Jun
AU  - Wang HJ
AUID- ORCID: 0000-0001-5906-8980
AD  - Department of Anesthesiology, University of Nebraska Medical Center, Omaha, NE 
      68198-5850, USA. hanjunwang@unmc.edu.
LA  - eng
GR  - R01 HL121012/HL/NHLBI NIH HHS/United States
GR  - R01 HL126796/HL/NHLBI NIH HHS/United States
GR  - R01HL121012/NH/NIH HHS/United States
PT  - Journal Article
DEP - 20190324
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/pharmacology
MH  - Body Weight
MH  - Brain-Derived Neurotrophic Factor/antagonists & inhibitors/genetics/*metabolism
MH  - Disease Models, Animal
MH  - Ganglia, Spinal/*metabolism
MH  - Heart Failure/diagnosis/*etiology/*physiopathology
MH  - Hemodynamics
MH  - Immunohistochemistry
MH  - Lumbosacral Region
MH  - Male
MH  - Organ Size
MH  - *Physical Conditioning, Animal
MH  - Rats
MH  - *Reflex/drug effects
MH  - Sensory Receptor Cells/metabolism
PMC - PMC6471760
OTO - NOTNLM
OT  - cardiovascular reflexes
OT  - exercise
OT  - mechanoreflex
OT  - neurotrophic factors
COIS- The authors declare no conflicts of interest.
EDAT- 2019/03/27 06:00
MHDA- 2019/07/18 06:00
PMCR- 2019/03/01
CRDT- 2019/03/27 06:00
PHST- 2019/01/19 00:00 [received]
PHST- 2019/03/20 00:00 [revised]
PHST- 2019/03/21 00:00 [accepted]
PHST- 2019/03/27 06:00 [entrez]
PHST- 2019/03/27 06:00 [pubmed]
PHST- 2019/07/18 06:00 [medline]
PHST- 2019/03/01 00:00 [pmc-release]
AID - ijms20061480 [pii]
AID - ijms-20-01480 [pii]
AID - 10.3390/ijms20061480 [doi]
PST - epublish
SO  - Int J Mol Sci. 2019 Mar 24;20(6):1480. doi: 10.3390/ijms20061480.