PMID- 30909752
OWN - NLM
STAT- MEDLINE
DCOM- 20200828
LR  - 20200828
IS  - 1460-2202 (Electronic)
IS  - 0271-3683 (Linking)
VI  - 44
IP  - 8
DP  - 2019 Aug
TI  - Correlation between Guttata Severity and Thickness of Descemet's Membrane and the 
      Central Cornea.
PG  - 849-855
LID - 10.1080/02713683.2019.1600194 [doi]
AB  - Purpose: To characterize and correlate guttata severity, Descemet's membrane 
      thickness (DMT), central cornea thickness (CCT) in corneas with guttae using 
      specular microscopy and spectral-domain optical coherence tomography (SD-OCT) and 
      test the Doheny Image Reading Center (DIRC) specular microscopy-based corneal 
      guttata severity scale. Methods: Forty-nine eyes of 49 patients with guttata and 
      36 eyes of age-matched of 36 normal controls were enrolled in the study. Three 
      images of the central cornea and four of the peripheral cornea (inferior, 
      superior, nasal and temporal) of each eye were taken using the Konan NSP-9900 
      specular microscope. A volume scan of the central cornea cross-section was 
      collected on each eye using the Heidelberg Spectralis SD-OCT. The density of 
      endothelial guttata based on specular images was graded on a 0-4 scale, and the 
      Descemet's membrane thickness (DMT) and central corneal thickness (CCT) were 
      manually measured by two trained graders. Results: The DIRC corneal guttata 
      severity scale showed good reproducibility of all corneal endothelial images 
      (weighted Kappa = 0.87). Mean DMT was 16.1 +/- 2.4 microm in controls and 25.5 +/- 10.9 
      microm in corneas with guttata (P < 0.001). Mean CCT was 552 +/- 26 microm in controls and 
      603 +/- 55 microm in corneas with guttata (P < 0.001). Guttata severity was 
      significantly correlated with both DMT (r = 0.743, P < 0.001) and CCT (r = 0.569, 
      P < 0.001). Age was moderately correlated with DMT (r = 0.472, P = 0.003) and 
      mildly correlated with guttata severity (r = 0.285, P = 0.031), but was not 
      correlated with CCT (r = 0.058, P = 0.681). Guttatta grade 3 corneas displayed an 
      increase in DMT and guttata grade 4 was associated with a significant increase in 
      CCT. Conclusions: The DMT and CCT are increased in corneas with guttata. The 
      higher density of guttae is correlated with increased thickness. Specular 
      microscopy combined with SD-OCT can be used as a good approach to assess the 
      severity of FECD.
FAU - Huang, Jianyan
AU  - Huang J
AD  - a Doheny Image Reading Center, Doheny Eye Institute , Los Angeles , California , 
      USA.
AD  - b Department of Ophthalmology, David Geffen School of Medicine at UCLA , Los 
      Angeles , California , USA.
FAU - Tepelus, Tudor C
AU  - Tepelus TC
AD  - a Doheny Image Reading Center, Doheny Eye Institute , Los Angeles , California , 
      USA.
AD  - b Department of Ophthalmology, David Geffen School of Medicine at UCLA , Los 
      Angeles , California , USA.
FAU - Baghdasaryan, Elmira
AU  - Baghdasaryan E
AD  - a Doheny Image Reading Center, Doheny Eye Institute , Los Angeles , California , 
      USA.
AD  - b Department of Ophthalmology, David Geffen School of Medicine at UCLA , Los 
      Angeles , California , USA.
FAU - Huang, Ping
AU  - Huang P
AD  - a Doheny Image Reading Center, Doheny Eye Institute , Los Angeles , California , 
      USA.
AD  - b Department of Ophthalmology, David Geffen School of Medicine at UCLA , Los 
      Angeles , California , USA.
FAU - Shi, Yue
AU  - Shi Y
AD  - a Doheny Image Reading Center, Doheny Eye Institute , Los Angeles , California , 
      USA.
AD  - b Department of Ophthalmology, David Geffen School of Medicine at UCLA , Los 
      Angeles , California , USA.
FAU - Hsu, Hugo Y
AU  - Hsu HY
AD  - a Doheny Image Reading Center, Doheny Eye Institute , Los Angeles , California , 
      USA.
AD  - b Department of Ophthalmology, David Geffen School of Medicine at UCLA , Los 
      Angeles , California , USA.
FAU - Sadda, Srinivas R
AU  - Sadda SR
AD  - a Doheny Image Reading Center, Doheny Eye Institute , Los Angeles , California , 
      USA.
AD  - b Department of Ophthalmology, David Geffen School of Medicine at UCLA , Los 
      Angeles , California , USA.
FAU - Lee, Olivia L
AU  - Lee OL
AD  - a Doheny Image Reading Center, Doheny Eye Institute , Los Angeles , California , 
      USA.
AD  - b Department of Ophthalmology, David Geffen School of Medicine at UCLA , Los 
      Angeles , California , USA.
LA  - eng
PT  - Journal Article
DEP - 20190405
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Cornea/*pathology
MH  - Corneal Diseases/diagnostic imaging/*pathology
MH  - Descemet Membrane/diagnostic imaging/*pathology
MH  - Endothelium, Corneal/diagnostic imaging/*pathology
MH  - Female
MH  - Humans
MH  - Male
MH  - Microscopy
MH  - Middle Aged
MH  - Organ Size
MH  - Reproducibility of Results
MH  - Tomography, Optical Coherence
OTO - NOTNLM
OT  - Descemet's membrane thickness
OT  - Fuchs' endothelial corneal dystrophy
OT  - Guttata density
OT  - corneal guttata
OT  - spectral-domain optical coherence tomography
OT  - specular microscopy
EDAT- 2019/03/27 06:00
MHDA- 2020/08/29 06:00
CRDT- 2019/03/27 06:00
PHST- 2019/03/27 06:00 [pubmed]
PHST- 2020/08/29 06:00 [medline]
PHST- 2019/03/27 06:00 [entrez]
AID - 10.1080/02713683.2019.1600194 [doi]
PST - ppublish
SO  - Curr Eye Res. 2019 Aug;44(8):849-855. doi: 10.1080/02713683.2019.1600194. Epub 
      2019 Apr 5.