PMID- 30910720
OWN - NLM
STAT- MEDLINE
DCOM- 20190729
LR  - 20190729
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 131
DP  - 2019 Jun
TI  - Rumen-derived lipopolysaccharide induced ruminal epithelium barrier damage in 
      goats fed a high-concentrate diet.
PG  - 81-86
LID - S0882-4010(18)31084-2 [pii]
LID - 10.1016/j.micpath.2019.02.007 [doi]
AB  - This study aimed to investigate the mechanism of lipopolysaccharide (LPS) 
      released in the rumen on epithelium barrier function of goats fed a HC diet. 
      Twelve Boer goats were randomly divided into two groups: low-concentrate(LC) diet 
      and high-concentrate(HC) diet treatment. We found that the pH of rumen fluid in 
      the HC group was lower than in the LC group (P < 0.05). The mRNA and protein 
      expression levels of p38 mitogen-activated protein kinase (MAPK), extracellular 
      regulated protein kinases (ERK), and c-Jun N-terminal kinase (JNK) in the rumen 
      epithelium were lower in the LC group than the HC group (P < 0.05). Gene 
      expression and protein levels of the tight junction proteins claudin-1, 
      claudin-4, occludin, and Zona occludin-1 were all greater in the LC group than 
      the HC group (P < 0.05). Staining of claudin-1, occludin and ZO-1 was became 
      irregular. In conclusion, high concentrate diet feeding can impair rumen 
      epithelium function and decrease tight junction protein expression through MAPK 
      signaling pathway.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Zhang, Kai
AU  - Zhang K
AD  - Circular Agriculture Research Center, Jiangsu Academy of Agricultural Sciences, 
      Nanjing, China; Key Laboratory of Crop and Livestock Integrated Farming, Ministry 
      of Agriculture, Nanjing, China.
FAU - Meng, Meijuan
AU  - Meng M
AD  - Circular Agriculture Research Center, Jiangsu Academy of Agricultural Sciences, 
      Nanjing, China; Key Laboratory of Crop and Livestock Integrated Farming, Ministry 
      of Agriculture, Nanjing, China.
FAU - Gao, Lipeng
AU  - Gao L
AD  - Circular Agriculture Research Center, Jiangsu Academy of Agricultural Sciences, 
      Nanjing, China; Key Laboratory of Crop and Livestock Integrated Farming, Ministry 
      of Agriculture, Nanjing, China.
FAU - Tu, Yuanlu
AU  - Tu Y
AD  - Circular Agriculture Research Center, Jiangsu Academy of Agricultural Sciences, 
      Nanjing, China; Key Laboratory of Crop and Livestock Integrated Farming, Ministry 
      of Agriculture, Nanjing, China.
FAU - Bai, Yunfeng
AU  - Bai Y
AD  - Circular Agriculture Research Center, Jiangsu Academy of Agricultural Sciences, 
      Nanjing, China; Key Laboratory of Crop and Livestock Integrated Farming, Ministry 
      of Agriculture, Nanjing, China. Electronic address: blinkeye@126.com.
LA  - eng
PT  - Clinical Trial, Veterinary
PT  - Journal Article
DEP - 20190322
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Claudin-1)
RN  - 0 (Claudin-4)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Occludin)
RN  - 0 (Tight Junction Proteins)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
MH  - Animal Feed/analysis
MH  - Animals
MH  - Claudin-1/genetics/metabolism
MH  - Claudin-4/genetics/metabolism
MH  - Diet/*veterinary
MH  - Epithelium/*metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Gene Expression Regulation
MH  - Goats
MH  - Hydrogen-Ion Concentration
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - Lipopolysaccharides/*metabolism
MH  - Occludin/genetics/metabolism
MH  - Random Allocation
MH  - Rumen/*metabolism
MH  - Signal Transduction
MH  - Tight Junction Proteins/genetics/metabolism
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
OTO - NOTNLM
OT  - Goat
OT  - Lipopolysaccharide
OT  - Rumen epithelium
OT  - Subacute rumen acidosis
OT  - Tight junction proteins
EDAT- 2019/03/27 06:00
MHDA- 2019/07/30 06:00
CRDT- 2019/03/27 06:00
PHST- 2018/06/11 00:00 [received]
PHST- 2019/02/01 00:00 [revised]
PHST- 2019/02/04 00:00 [accepted]
PHST- 2019/03/27 06:00 [pubmed]
PHST- 2019/07/30 06:00 [medline]
PHST- 2019/03/27 06:00 [entrez]
AID - S0882-4010(18)31084-2 [pii]
AID - 10.1016/j.micpath.2019.02.007 [doi]
PST - ppublish
SO  - Microb Pathog. 2019 Jun;131:81-86. doi: 10.1016/j.micpath.2019.02.007. Epub 2019 
      Mar 22.