PMID- 30911071
OWN - NLM
STAT- MEDLINE
DCOM- 20200930
LR  - 20210109
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Mar 25
TI  - Kisspeptin/kisspeptin receptor system in pseudopregnant rabbit corpora lutea: 
      presence and function.
PG  - 5044
LID - 10.1038/s41598-019-41623-1 [doi]
LID - 5044
AB  - Kisspeptin (KiSS) and its related receptors (KiSS1R) have a critical role in the 
      reproduction of mammals. The KiSS/KiSS1R system is expressed in numerous 
      reproductive organs including the ovary. Here, we studied the expression of the 
      KiSS/KiSS1R system and its functional role in rabbit corpora lutea (CL) at days 4 
      (early-), 9 (mid-), and 13 (late-stage) of pseudopregnancy. In vitro 
      progesterone, prostaglandin (PG) F2alpha (PGF2alpha) and E2 (PGE2) productions and 
      prostaglandin-endoperoxide synthase 1 (PTGS1) and 2 (PTGS2) activities were 
      evaluated. Immune reactivity (IR) for KiSS and KiSS1R were detected in luteal 
      cells at nuclear and cytoplasmic level at all luteal stage for KiSS and only at 
      early- and mid-stage for KiSS1R; IR decreased from early- to later stages of 
      pseudopregnancy. The KiSS-10 augmented progesterone and PGE2 and diminished PGF2alpha 
      secretions by early- and mid-CL; KiSS-10 reduced PTGS2 activity at early- and 
      mid-stages, but did not affect PTGS1 at any luteal stages. The antagonist 
      KiSS-234 counteracted all KiSS-10 effects. This study shows that the KiSS/KiSS1R 
      system is expressed in CL of pseudopregnant rabbits and exerts a luteotropic 
      action by down-regulating PTGS2, which decreases PGF2alpha and increases PGE2 and 
      progesterone.
FAU - Maranesi, Margherita
AU  - Maranesi M
AD  - Dipartimento di Medicina veterinaria, Universita di Perugia, via San Costanzo 4, 
      Perugia, IT, 06126, Italy.
FAU - Petrucci, Linda
AU  - Petrucci L
AD  - Dipartimento di Medicina veterinaria, Universita di Perugia, via San Costanzo 4, 
      Perugia, IT, 06126, Italy.
FAU - Leonardi, Leonardo
AU  - Leonardi L
AD  - Dipartimento di Medicina veterinaria, Universita di Perugia, via San Costanzo 4, 
      Perugia, IT, 06126, Italy.
FAU - Bufalari, Antonello
AU  - Bufalari A
AD  - Dipartimento di Medicina veterinaria, Universita di Perugia, via San Costanzo 4, 
      Perugia, IT, 06126, Italy.
FAU - Parillo, Francesco
AU  - Parillo F
AD  - Scuola di Bioscienze e Medicina veterinaria, Universita di Camerino, via 
      Circonvallazione 93, Matelica, IT, 62024, Italy.
FAU - Boiti, Cristiano
AU  - Boiti C
AD  - Dipartimento di Medicina veterinaria, Universita di Perugia, via San Costanzo 4, 
      Perugia, IT, 06126, Italy.
FAU - Zerani, Massimo
AU  - Zerani M
AUID- ORCID: 0000-0002-2476-5097
AD  - Dipartimento di Medicina veterinaria, Universita di Perugia, via San Costanzo 4, 
      Perugia, IT, 06126, Italy. massimo.zerani@unicam.it.
AD  - Scuola di Bioscienze e Medicina veterinaria, Universita di Camerino, via 
      Circonvallazione 93, Matelica, IT, 62024, Italy. massimo.zerani@unicam.it.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190325
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Kisspeptins)
RN  - 0 (Receptors, Kisspeptin-1)
RN  - B7IN85G1HY (Dinoprost)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Cyclooxygenase 2/metabolism
MH  - Dinoprost/metabolism
MH  - Dinoprostone/metabolism
MH  - Female
MH  - Kisspeptins/*biosynthesis
MH  - Luteal Cells/*metabolism/pathology
MH  - Pregnancy
MH  - Pseudopregnancy/*metabolism/pathology
MH  - Rabbits
MH  - Receptors, Kisspeptin-1/*biosynthesis
PMC - PMC6433948
COIS- The authors declare no competing interests.
EDAT- 2019/03/27 06:00
MHDA- 2020/10/02 06:00
PMCR- 2019/03/25
CRDT- 2019/03/27 06:00
PHST- 2018/07/09 00:00 [received]
PHST- 2019/03/11 00:00 [accepted]
PHST- 2019/03/27 06:00 [entrez]
PHST- 2019/03/27 06:00 [pubmed]
PHST- 2020/10/02 06:00 [medline]
PHST- 2019/03/25 00:00 [pmc-release]
AID - 10.1038/s41598-019-41623-1 [pii]
AID - 41623 [pii]
AID - 10.1038/s41598-019-41623-1 [doi]
PST - epublish
SO  - Sci Rep. 2019 Mar 25;9(1):5044. doi: 10.1038/s41598-019-41623-1.