PMID- 30911278
OWN - NLM
STAT- MEDLINE
DCOM- 20190724
LR  - 20200225
IS  - 1449-1907 (Electronic)
IS  - 1449-1907 (Linking)
VI  - 16
IP  - 3
DP  - 2019
TI  - Decreased Ambient Oxygen Tension Alters the Expression of Endothelin-1, iNOS and 
      cGMP in Rat Alveolar Macrophages.
PG  - 443-449
LID - 10.7150/ijms.28353 [doi]
AB  - Background: Hypoxia plays an important role in the vascular tone of pulmonary 
      circulation via the vasculature and parenchymal tissue. Endothelin-1 (ET-1), a 
      potent vasoconstrictive peptide, plays a role in inflammation in mononuclear 
      cells. Nitric oxide synthase (NOS), which generates nitric oxide (NO)/cyclic 3', 
      5'-monophosphate (cGMP), is coexpressed with ET-1 in many cell types. The aim of 
      this study was to assess whether hypoxia induces the production of ET-1 and 
      associated expression of NOS, NO/cGMP and chemokines in rat alveolar macrophages 
      (AMs). Methods: NR8383 cells were cultured under hypoxic (1% oxygen) conditions 
      for 0, 2, 4, 8 and 12 hours. Levels of ET-1, inducible NOS (iNOS), phosphorylated 
      iNOS (p-iNOS), nitrite/nitrate (NOx), cGMP and monocyte chemoattractant protein-1 
      (MCP-1) were measured. Results: ET-1, p-iNOS, NOx, and cGMP increased 
      significantly in AMs after 4 hours of hypoxia (p < 0.05). ET-1 and MCP-1 mRNA 
      increased after 8 hours (p < 0.05). The protein expression of ET-1, MCP-1, and 
      p-iNOS increased in a time-dependent manner, while iNOS expression decreased with 
      time. Conclusions: The changes in ET-1, p-iNOS, and the NO/cGMP pathway in AMs 
      may help elucidate the mechanisms in the hypoxic lung. Understanding changes in 
      the endothelin axis in hypoxic AMs is a crucial first step to unravel its role in 
      pulmonary circulation.
FAU - Chen, I-Chen
AU  - Chen IC
AD  - Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung, 
      Taiwan.
AD  - Department of Pediatrics, School of Medicine, College of Medicine, Kaohsiung 
      Medical University, Kaohsiung, Taiwan.
AD  - Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical 
      University, Kaohsiung, Taiwan.
FAU - Lin, Yu-Tsai
AU  - Lin YT
AD  - Department of Otolaryngology, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, 
      Taiwan.
FAU - Huang, Jhy-Shrian
AU  - Huang JS
AD  - Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung, 
      Taiwan.
FAU - Wu, Bin-Nan
AU  - Wu BN
AD  - Department of Pharmacology, School of Medicine, College of Medicine, Kaohsiung 
      Medical University, Kaohsiung, Taiwan.
FAU - Hsu, Jong-Hau
AU  - Hsu JH
AD  - Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung, 
      Taiwan.
AD  - Department of Pediatrics, School of Medicine, College of Medicine, Kaohsiung 
      Medical University, Kaohsiung, Taiwan.
FAU - Tan, Mian-Shin
AU  - Tan MS
AD  - Department of Biomedical Science and Environmental Biology, College of Life 
      Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan.
FAU - Dai, Zen-Kong
AU  - Dai ZK
AD  - Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung, 
      Taiwan.
AD  - Department of Pediatrics, School of Medicine, College of Medicine, Kaohsiung 
      Medical University, Kaohsiung, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20190228
PL  - Australia
TA  - Int J Med Sci
JT  - International journal of medical sciences
JID - 101213954
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Endothelin-1)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, rat)
RN  - H2D2X058MU (Cyclic GMP)
MH  - Animals
MH  - Cell Hypoxia
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/metabolism
MH  - Cyclic GMP/*metabolism
MH  - Endothelin-1/genetics/*metabolism
MH  - Gene Expression Regulation
MH  - Macrophages, Alveolar/*metabolism
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase Type II/genetics/*metabolism
MH  - Rats, Sprague-Dawley
PMC - PMC6428981
OTO - NOTNLM
OT  - alveolar macrophage
OT  - endothelin-1
OT  - hypoxia
OT  - nitric oxide synthase
COIS- Competing Interests: The authors have declared that no competing interest exists.
EDAT- 2019/03/27 06:00
MHDA- 2019/07/25 06:00
PMCR- 2019/01/01
CRDT- 2019/03/27 06:00
PHST- 2018/07/06 00:00 [received]
PHST- 2018/12/28 00:00 [accepted]
PHST- 2019/03/27 06:00 [entrez]
PHST- 2019/03/27 06:00 [pubmed]
PHST- 2019/07/25 06:00 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - ijmsv16p0443 [pii]
AID - 10.7150/ijms.28353 [doi]
PST - epublish
SO  - Int J Med Sci. 2019 Feb 28;16(3):443-449. doi: 10.7150/ijms.28353. eCollection 
      2019.