PMID- 30912030
OWN - NLM
STAT- MEDLINE
DCOM- 20190717
LR  - 20190717
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1953
DP  - 2019
TI  - Chromogenic Tissue-Based Methods for Detection of Gene Amplifications (or 
      Rearrangements) Combined with Protein Overexpression in Clinical Samples.
PG  - 301-314
LID - 10.1007/978-1-4939-9145-7_19 [doi]
AB  - Immunohistochemistry (IHC) is a well-established, tissue-based assay for the 
      visualization of target proteins. For analysis of DNA targets, chromogenic in 
      situ hybridization (CISH) applications have significant advantages over 
      traditional fluorescence in situ hybridization (FISH). CISH slides can be 
      analyzed using a regular light microscope, while FISH slides require the use of a 
      specialized fluorescence microscope in a dark room. CISH slides allow observers 
      to correlate the gene status (gene amplifications, gene rearrangements, and gene 
      deletions) in the context of tissue morphology better than FISH slides. Recently, 
      a combination of IHC and CISH assays (gene-protein assay, GPA) was developed to 
      study the relationship between gene status and protein expression on the same 
      tissue section. CISH and GPA applications can be optimized using an automated 
      tissue slide processing system to generate reproducible results for a long and 
      complex assay protocol. GPA applications are an ideal approach for tumor status 
      and heterogeneity analyses for research and clinical investigations.
FAU - Nitta, Hiroaki
AU  - Nitta H
AD  - Roche Tissue Diagnostics, Tucson, AZ, USA. hiro.nitta@roche.com.
FAU - Kelly, Brian
AU  - Kelly B
AD  - Roche Tissue Diagnostics, Tucson, AZ, USA.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Chromogenic Compounds)
RN  - 0 (Proteins)
SB  - IM
MH  - Chromogenic Compounds/analysis
MH  - *Gene Amplification
MH  - *Gene Rearrangement
MH  - Humans
MH  - Immunohistochemistry/*methods
MH  - In Situ Hybridization/*methods
MH  - Neoplasms/*genetics
MH  - Proteins/genetics
MH  - *Up-Regulation
OTO - NOTNLM
OT  - Cancer
OT  - Gene amplification
OT  - Gene rearrangement
OT  - Gene-protein assay
OT  - Personalized health care
OT  - Protein overexpression
EDAT- 2019/03/27 06:00
MHDA- 2019/07/18 06:00
CRDT- 2019/03/27 06:00
PHST- 2019/03/27 06:00 [entrez]
PHST- 2019/03/27 06:00 [pubmed]
PHST- 2019/07/18 06:00 [medline]
AID - 10.1007/978-1-4939-9145-7_19 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2019;1953:301-314. doi: 10.1007/978-1-4939-9145-7_19.