PMID- 30916574
OWN - NLM
STAT- MEDLINE
DCOM- 20200206
LR  - 20231213
IS  - 1557-8852 (Electronic)
IS  - 1084-9785 (Linking)
VI  - 34
IP  - 6
DP  - 2019 Aug
TI  - Pyrroline-5-Carboxylate Reductase 1 Accelerates the Migration and Invasion of 
      Nonsmall Cell Lung Cancer In Vitro.
PG  - 380-387
LID - 10.1089/cbr.2019.2782 [doi]
AB  - Background: Pyrroline-5-carboxylate reductase 1 (PYCR1) is involved in tumor 
      progression, for instance, breast cancer and prostate cancer. However, its role 
      in tumor metastasis, especially in nonsmall cell lung cancer (NSCLC), is still 
      elusive. Materials and Methods: The messenger RNA (mRNA) expression of PYCR1 
      between NSCLC and normal lung specimens was compared using Oncomine database. The 
      endogenous PYCR1 expressions in NSCLC cell lines 95C and H1299 were knocked down 
      by lentiviral-mediated delivery of short hairpin RNA (shRNA). Then the effects of 
      PYCR1 on the migration and invasion of NSCLC cells were studied by wound healing 
      assay and transwell assay. Results: PYCR1 mRNA expression was significantly 
      higher in NSCLC specimens than that in normal lung tissues. Depletion of PYCR1 in 
      NSCLC cell significantly repressed the cell migration and invasion. Moreover, 
      depletion of PYCR1 influenced the expression of epithelial-mesenchymal transition 
      molecules E-cadherin, Vimentin, N-cadherin, and Snail1. Conclusions: Our data 
      suggested that PYCR1 plays a positive role in NSCLC metastasis in vitro and might 
      be a promising target for treating NSCLC.
FAU - Sang, Senhua
AU  - Sang S
AD  - 1College of Life Science and Technology, Guangxi University, Nanning, China.
AD  - 2Shanghai Linger Biotechnology Co., Ltd., Shanghai, China.
FAU - Zhang, Cuicui
AU  - Zhang C
AD  - 1College of Life Science and Technology, Guangxi University, Nanning, China.
FAU - Shan, Jianwei
AU  - Shan J
AD  - 1College of Life Science and Technology, Guangxi University, Nanning, China.
AD  - 3Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 
      China.
AD  - 4Guangdong Provincial Key Laboratory of Crop Genetic Improvement, Guangzhou, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20190327
PL  - United States
TA  - Cancer Biother Radiopharm
JT  - Cancer biotherapy & radiopharmaceuticals
JID - 9605408
RN  - EC 1.5.1.- (Pyrroline Carboxylate Reductases)
SB  - IM
MH  - Apoptosis
MH  - Carcinoma, Non-Small-Cell Lung/genetics/metabolism/*pathology
MH  - *Cell Movement
MH  - Cell Proliferation
MH  - *Epithelial-Mesenchymal Transition
MH  - Humans
MH  - In Vitro Techniques
MH  - Lung Neoplasms/genetics/metabolism/*pathology
MH  - Neoplasm Invasiveness
MH  - Pyrroline Carboxylate Reductases/genetics/*metabolism
MH  - Tumor Cells, Cultured
MH  - delta-1-Pyrroline-5-Carboxylate Reductase
OTO - NOTNLM
OT  - PYCR1
OT  - invasion
OT  - migration
OT  - nonsmall cell lung cancer
OT  - shRNA
EDAT- 2019/03/28 06:00
MHDA- 2020/02/07 06:00
CRDT- 2019/03/28 06:00
PHST- 2019/03/28 06:00 [pubmed]
PHST- 2020/02/07 06:00 [medline]
PHST- 2019/03/28 06:00 [entrez]
AID - 10.1089/cbr.2019.2782 [doi]
PST - ppublish
SO  - Cancer Biother Radiopharm. 2019 Aug;34(6):380-387. doi: 10.1089/cbr.2019.2782. 
      Epub 2019 Mar 27.