PMID- 30916577
OWN - NLM
STAT- MEDLINE
DCOM- 20200219
LR  - 20200930
IS  - 1522-1490 (Electronic)
IS  - 0363-6119 (Linking)
VI  - 316
IP  - 6
DP  - 2019 Jun 1
TI  - Evidence for a role of arginine vasotocin receptors in the gill during salinity 
      acclimation by a euryhaline teleost fish.
PG  - R735-R750
LID - 10.1152/ajpregu.00328.2018 [doi]
AB  - The nonapeptide arginine vasotocin (AVT) regulates osmotic balance in teleost 
      fishes, but its mechanisms of action are not fully understood. Recently, it was 
      discovered that nonapeptide receptors in teleost fishes are differentiated into 
      two V1a-type, several V2-type, and two isotocin (IT) receptors, but it remains 
      unclear which receptors mediate AVT's effects on gill osmoregulation. Here, we 
      examined the role of nonapeptide receptors in the gill of the euryhaline Amargosa 
      pupfish (Cyprinodon nevadensis amargosae) during osmotic acclimation. Transcripts 
      for the teleost V1a-type receptor v1a2 were upregulated over fourfold in gill 24 
      h after transferring pupfish from 7.5 ppt to seawater (35 ppt) or hypersaline (55 
      ppt) conditions and downregulated after transfer to freshwater (0.3 ppt). Gill 
      transcripts for the nonapeptide degradation enzyme leucyl-cystinyl aminopeptidase 
      (LNPEP) also increased in fish acclimating to 35 ppt. To test whether the effects 
      of AVT on the gill might be mediated by a V1a-type receptor, we administered AVT 
      or a V1-type receptor antagonist (Manning compound) intraperitoneally to pupfish 
      before transfer to 0.4 ppt or 35 ppt. Pupfish transferred to 35 ppt exhibited 
      elevated gill mRNA abundance for cystic fibrosis transmembrane conductance 
      regulator (cftr), but that upregulation diminished under V1-receptor inhibition. 
      AVT inhibited the increase in gill Na(+)/Cl(-) cotransporter 2 (ncc2) transcript 
      abundance that occurs following transfer to hypoosmotic environments, whereas 
      V1-type receptor antagonism increased ncc2 mRNAs even without a change in 
      salinity. These findings indicate that AVT acts via a V1-type receptor to 
      regulate gill Cl(-) transport by inhibiting Cl(-) uptake and facilitating Cl(-) 
      secretion during seawater acclimation.
FAU - Lema, Sean C
AU  - Lema SC
AD  - Biological Sciences Department, Center for Coastal Marine Sciences, California 
      Polytechnic State University , San Luis Obispo, California.
FAU - Washburn, Elsie H
AU  - Washburn EH
AD  - Biological Sciences Department, Center for Coastal Marine Sciences, California 
      Polytechnic State University , San Luis Obispo, California.
FAU - Crowley, Mary E
AU  - Crowley ME
AD  - Biological Sciences Department, Center for Coastal Marine Sciences, California 
      Polytechnic State University , San Luis Obispo, California.
FAU - Carvalho, Paul G
AU  - Carvalho PG
AD  - Biological Sciences Department, Center for Coastal Marine Sciences, California 
      Polytechnic State University , San Luis Obispo, California.
FAU - Egelston, Jennifer N
AU  - Egelston JN
AD  - Biological Sciences Department, Center for Coastal Marine Sciences, California 
      Polytechnic State University , San Luis Obispo, California.
FAU - McCormick, Stephen D
AU  - McCormick SD
AD  - United States Geological Survey, Leetown Science Center, Conte Anadromous Fish 
      Research Laboratory, Turners Falls, Massachusetts.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190327
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (Fish Proteins)
RN  - 0 (Receptors, Vasopressin)
RN  - 0 (Solute Carrier Family 12, Member 1)
RN  - 0 (vasotocin receptor)
RN  - 126880-72-6 (Cystic Fibrosis Transmembrane Conductance Regulator)
RN  - 50-56-6 (Oxytocin)
RN  - 550-21-0 (isotocin)
RN  - EC 3.4.11.3 (Cystinyl Aminopeptidase)
RN  - EC 3.4.11.3 (leucyl-cystinyl aminopeptidase)
RN  - W6S6URY8OF (Vasotocin)
SB  - IM
MH  - Animals
MH  - Cystic Fibrosis Transmembrane Conductance Regulator/genetics/metabolism
MH  - Cystinyl Aminopeptidase/genetics/metabolism
MH  - Female
MH  - Fish Proteins/genetics/*metabolism
MH  - Gills/*metabolism
MH  - Killifishes/genetics/*metabolism
MH  - Male
MH  - *Osmoregulation
MH  - Oxytocin/analogs & derivatives/metabolism
MH  - Receptors, Vasopressin/genetics/*metabolism
MH  - *Salinity
MH  - *Salt Tolerance
MH  - Seawater
MH  - Signal Transduction
MH  - Solute Carrier Family 12, Member 1/genetics/metabolism
MH  - Up-Regulation
MH  - Vasotocin/*metabolism
OTO - NOTNLM
OT  - LNPEP
OT  - ion transport
OT  - isotocin
OT  - nonapeptide
OT  - pupfish
OT  - vasopressin
EDAT- 2019/03/28 06:00
MHDA- 2020/02/20 06:00
CRDT- 2019/03/28 06:00
PHST- 2019/03/28 06:00 [pubmed]
PHST- 2020/02/20 06:00 [medline]
PHST- 2019/03/28 06:00 [entrez]
AID - 10.1152/ajpregu.00328.2018 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2019 Jun 1;316(6):R735-R750. doi: 
      10.1152/ajpregu.00328.2018. Epub 2019 Mar 27.