PMID- 30916807
OWN - NLM
STAT- MEDLINE
DCOM- 20190530
LR  - 20190530
IS  - 1600-0463 (Electronic)
IS  - 0903-4641 (Linking)
VI  - 127
IP  - 6
DP  - 2019 Jun
TI  - The synthetic antimicrobial peptide LTX21 induces inflammatory responses in a 
      human whole blood model and a murine peritoneum model.
PG  - 475-483
LID - 10.1111/apm.12946 [doi]
AB  - The global spread of antimicrobial resistance and the increasing number of 
      immune-compromised patients are major challenges in modern medicine. Targeting 
      bacterial virulence or the human host immune system to increase host defence are 
      important strategies in the search for novel antimicrobial drugs. We investigated 
      the inflammatory response of the synthetic short antimicrobial peptide LTX21 in 
      two model systems: a human whole blood ex vivo model and a murine in vivo 
      peritoneum model - both reflecting early innate immune response. In the whole 
      blood model, LTX21 increased the secretion of a range of different cytokines, 
      decreased the level of tumour necrosis factor (TNF) and activated the complement 
      system. In a haemolysis assay, we found 2.5% haemolysis at a LTX21 concentration 
      of 500 mg/L. In the murine model, increased influx of white blood cells (WBCs) 
      and polymorphonuclear neutrophils (PMNs) in the murine peritoneal cavity was 
      observed after treatment with LTX21. In addition, LTX21 increased monocyte 
      chemoattractant protein-1 (MCP-1). In conclusion, LTX21 affected the inflammatory 
      response; the increase in cytokine secretion, complement activation and WBC 
      influx indicates an activated inflammatory response. The present results indicate 
      the impact of LTX21 on the host-pathogen interplay. Whether this will also affect 
      the course of infection has to be investigated.
CI  - (c) 2019 APMIS. Published by John Wiley & Sons Ltd.
FAU - Granslo, Hildegunn Norbakken
AU  - Granslo HN
AD  - Paediatric Research Group, Department of Clinical Medicine, Faculty of Health 
      Sciences, UiT, The Arctic University of Norway, Tromso, Norway.
AD  - Department of Paediatrics, University Hospital of North Norway, Tromso, Norway.
FAU - Aarag Fredheim, Elizabeth G
AU  - Aarag Fredheim EG
AD  - Paediatric Research Group, Department of Clinical Medicine, Faculty of Health 
      Sciences, UiT, The Arctic University of Norway, Tromso, Norway.
AD  - Microbial Pharmacology and Population Ecology, Department of Pharmacy, Faculty of 
      Health Sciences, UiT, The Arctic University of Norway, Tromso, Norway.
FAU - Esaiassen, Eirin
AU  - Esaiassen E
AD  - Paediatric Research Group, Department of Clinical Medicine, Faculty of Health 
      Sciences, UiT, The Arctic University of Norway, Tromso, Norway.
AD  - Department of Paediatrics, University Hospital of North Norway, Tromso, Norway.
FAU - Christophersen, Lars
AU  - Christophersen L
AD  - Department of Clinical Microbiology, Copenhagen University Hospital, 
      Rigshospitalet, Copenhagen, Denmark.
FAU - Jensen, Peter Ostrup
AU  - Jensen PO
AD  - Department of Clinical Microbiology, Copenhagen University Hospital, 
      Rigshospitalet, Copenhagen, Denmark.
FAU - Mollnes, Tom Eirik
AU  - Mollnes TE
AD  - Research Laboratory, Nordland Hospital, Bodo, Norway.
AD  - Department of Immunology, Oslo University Hospital, Norway.
AD  - Centre of Molecular Inflammation Research, Norwegian University of Science and 
      Technology, Trondheim, Norway.
FAU - Moser, Claus
AU  - Moser C
AD  - Department of Clinical Microbiology, Copenhagen University Hospital, 
      Rigshospitalet, Copenhagen, Denmark.
FAU - Flaegstad, Trond
AU  - Flaegstad T
AD  - Paediatric Research Group, Department of Clinical Medicine, Faculty of Health 
      Sciences, UiT, The Arctic University of Norway, Tromso, Norway.
AD  - Department of Paediatrics, University Hospital of North Norway, Tromso, Norway.
FAU - Klingenberg, Claus
AU  - Klingenberg C
AD  - Paediatric Research Group, Department of Clinical Medicine, Faculty of Health 
      Sciences, UiT, The Arctic University of Norway, Tromso, Norway.
AD  - Department of Paediatrics, University Hospital of North Norway, Tromso, Norway.
FAU - Cavanagh, Jorunn Pauline
AU  - Cavanagh JP
AD  - Paediatric Research Group, Department of Clinical Medicine, Faculty of Health 
      Sciences, UiT, The Arctic University of Norway, Tromso, Norway.
AD  - Department of Paediatrics, University Hospital of North Norway, Tromso, Norway.
LA  - eng
GR  - Northern Norway Regional Health Authority/
PT  - Journal Article
DEP - 20190426
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Cytokines)
RN  - 0 (Oligopeptides)
SB  - IM
MH  - Animals
MH  - Anti-Infective Agents/*pharmacology
MH  - Complement Activation/drug effects
MH  - Cytokines/biosynthesis
MH  - Female
MH  - Hemolysis/drug effects
MH  - Humans
MH  - Immunity, Innate/drug effects
MH  - Inflammation/*chemically induced
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oligopeptides/*pharmacology
OTO - NOTNLM
OT  - LTX21
OT  - cationic peptides
OT  - human whole blood model
OT  - murine model
EDAT- 2019/03/28 06:00
MHDA- 2019/05/31 06:00
CRDT- 2019/03/28 06:00
PHST- 2018/09/06 00:00 [received]
PHST- 2019/03/14 00:00 [accepted]
PHST- 2019/03/28 06:00 [pubmed]
PHST- 2019/05/31 06:00 [medline]
PHST- 2019/03/28 06:00 [entrez]
AID - 10.1111/apm.12946 [doi]
PST - ppublish
SO  - APMIS. 2019 Jun;127(6):475-483. doi: 10.1111/apm.12946. Epub 2019 Apr 26.