PMID- 3091782
OWN - NLM
STAT- MEDLINE
DCOM- 19861002
LR  - 20191101
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 6
IP  - 8
DP  - 1986 Aug
TI  - Structural similarities and differences between neurofilament proteins from five 
      different species as revealed using monoclonal antibodies.
PG  - 2179-86
AB  - Neurofilament (NF) proteins isolated from human, rabbit, rat, and chicken spinal 
      cord white matter were immunoblotted with monoclonal antibodies (MAbs) raised to 
      bovine NF immunogens. The aim of these experiments was to test the degree of 
      epitope conservation among NF proteins from different vertebrate species. In so 
      doing, the validity of using antibodies raised to NF from one species (cow) for 
      detecting NF proteins of other species was also tested. The MAbs used for these 
      experiments were characterized using bovine NFs to define polypeptide 
      specificity, the approximate location of epitopes within each NF polypeptide, and 
      the effects on antibody recognition of the presence or absence of phosphorylated 
      residues in these polypeptides (see Carden et al., 1985). Our findings indicate 
      that epitopes located in the alpha-helical core domains of NF-H, NF-M, and NF-L 
      are distinct from each other, yet are strongly conserved among the different 
      species. Epitopes located in the noncore or peripheral domains of the NF 
      polypeptides show variable degrees of cross-species preservation. For example, 
      all epitopes in the peripheral domains of bovine NF-H that require the presence 
      of phosphate groups for recognition (phosphorylation dependent) are widely 
      expressed in all species studied. Many phosphorylation-dependent epitopes of 
      bovine NF-H are also present in bovine NF-M, as well as in NF-H of non-bovine 
      species. In addition, epitopes in the peripheral domain of NF-H that require 
      dephosphorylation of NF polypeptides for recognition (dephosphorylation 
      dependent) are also found in NF-H of other mammalian species but not in NF-H of 
      chicken.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Lee, V M
AU  - Lee VM
FAU - Carden, M J
AU  - Carden MJ
FAU - Schlaepfer, W W
AU  - Schlaepfer WW
LA  - eng
GR  - NS-15572/NS/NINDS NIH HHS/United States
GR  - NS-18616/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Intermediate Filament Proteins)
RN  - 0 (Neurofilament Proteins)
SB  - IM
MH  - Animals
MH  - *Antibodies, Monoclonal
MH  - Antibody Specificity
MH  - Chickens
MH  - Cross Reactions
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - Immunosorbent Techniques
MH  - Intermediate Filament Proteins/*analysis
MH  - Molecular Weight
MH  - Neurofilament Proteins
MH  - Rabbits
MH  - Rats
MH  - Species Specificity
MH  - Spinal Cord/analysis
PMC - PMC6568742
EDAT- 1986/08/01 00:00
MHDA- 1986/08/01 00:01
PMCR- 1987/02/01
CRDT- 1986/08/01 00:00
PHST- 1986/08/01 00:00 [pubmed]
PHST- 1986/08/01 00:01 [medline]
PHST- 1986/08/01 00:00 [entrez]
PHST- 1987/02/01 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.06-08-02179.1986 [doi]
PST - ppublish
SO  - J Neurosci. 1986 Aug;6(8):2179-86. doi: 10.1523/JNEUROSCI.06-08-02179.1986.