PMID- 30918007
OWN - NLM
STAT- MEDLINE
DCOM- 20190903
LR  - 20200501
IS  - 1943-2631 (Electronic)
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 212
IP  - 1
DP  - 2019 May
TI  - Identification of rfk-1, a Meiotic Driver Undergoing RNA Editing in Neurospora.
PG  - 93-110
LID - 10.1534/genetics.119.302122 [doi]
AB  - Sk-2 is a meiotic drive element that was discovered in wild populations of 
      Neurospora fungi over 40 years ago. While early studies quickly determined that 
      Sk-2 transmits itself through sexual reproduction in a biased manner via spore 
      killing, the genetic factors responsible for this phenomenon have remained mostly 
      unknown. Here, we identify and characterize rfk-1, a gene required for Sk-2-based 
      spore killing. The rfk-1 gene contains four exons, three introns, and two stop 
      codons, the first of which undergoes RNA editing to a tryptophan codon during 
      sexual development. Translation of an unedited rfk-1 transcript in vegetative 
      tissue is expected to produce a 102-amino acid protein, whereas translation of an 
      edited rfk-1 transcript in sexual tissue is expected to produce a protein with 
      130 amino acids. These findings indicate that unedited and edited rfk-1 
      transcripts exist and that these transcripts could have different roles with 
      respect to the mechanism of meiotic drive by spore killing. Regardless of RNA 
      editing, spore killing only succeeds if rfk-1 transcripts avoid silencing caused 
      by a genome defense process called meiotic silencing by unpaired DNA (MSUD). We 
      show that rfk-1's MSUD avoidance mechanism is linked to the genomic landscape 
      surrounding the rfk-1 gene, which is located near the Sk-2 border on the right 
      arm of chromosome III. In addition to demonstrating that the location of rfk-1 is 
      critical to spore-killing success, our results add to accumulating evidence that 
      MSUD helps protect Neurospora genomes from complex meiotic drive elements.
CI  - Copyright (c) 2019 by the Genetics Society of America.
FAU - Rhoades, Nicholas A
AU  - Rhoades NA
AD  - School of Biological Sciences, Illinois State University, Normal, Illinois 61790.
FAU - Harvey, Austin M
AU  - Harvey AM
AD  - School of Biological Sciences, Illinois State University, Normal, Illinois 61790.
FAU - Samarajeewa, Dilini A
AU  - Samarajeewa DA
AD  - School of Biological Sciences, Illinois State University, Normal, Illinois 61790.
FAU - Svedberg, Jesper
AU  - Svedberg J
AD  - Department of Organismal Biology, Uppsala University, Norbyvagen 18D, 752 36 
      Uppsala, Sweden.
FAU - Yusifov, Aykhan
AU  - Yusifov A
AD  - School of Biological Sciences, Illinois State University, Normal, Illinois 61790.
FAU - Abusharekh, Anna
AU  - Abusharekh A
AD  - School of Biological Sciences, Illinois State University, Normal, Illinois 61790.
FAU - Manitchotpisit, Pennapa
AU  - Manitchotpisit P
AD  - School of Biological Sciences, Illinois State University, Normal, Illinois 61790.
FAU - Brown, Daren W
AU  - Brown DW
AD  - Mycotoxin Prevention and Applied Microbiology, National Center for Agricultural 
      Utilization Research, U.S. Department of Agriculture, Agricultural Research 
      Service, Peoria, Illinois 61604.
FAU - Sharp, Kevin J
AU  - Sharp KJ
AD  - School of Biological Sciences, Illinois State University, Normal, Illinois 61790.
FAU - Rehard, David G
AU  - Rehard DG
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 
      65211.
AD  - Department of Biology, University of Iowa, Iowa 52242.
FAU - Peters, Joshua
AU  - Peters J
AD  - School of Biological Sciences, Illinois State University, Normal, Illinois 61790.
FAU - Ostolaza-Maldonado, Xavier
AU  - Ostolaza-Maldonado X
AD  - School of Biological Sciences, Illinois State University, Normal, Illinois 61790.
FAU - Stephenson, Jackson
AU  - Stephenson J
AD  - School of Biological Sciences, Illinois State University, Normal, Illinois 61790.
FAU - Shiu, Patrick K T
AU  - Shiu PKT
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 
      65211.
FAU - Johannesson, Hanna
AU  - Johannesson H
AD  - Department of Organismal Biology, Uppsala University, Norbyvagen 18D, 752 36 
      Uppsala, Sweden.
FAU - Hammond, Thomas M
AU  - Hammond TM
AD  - School of Biological Sciences, Illinois State University, Normal, Illinois 61790 
      tmhammo@ilstu.edu.
LA  - eng
SI  - figshare/10.25386/genetics.7836692
GR  - P01 GM068087/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20190327
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (Fungal Proteins)
SB  - IM
MH  - Fungal Proteins/genetics/*metabolism
MH  - Gene Expression Regulation, Fungal
MH  - *Meiosis
MH  - Neurospora/genetics/*metabolism/physiology
MH  - *RNA Editing
MH  - Spores, Fungal/genetics/*metabolism
PMC - PMC6499513
OTO - NOTNLM
OT  - Genetics of Sex
OT  - RNA editing
OT  - meiotic drive
OT  - meiotic silencing
OT  - spore killing
OT  - transmission ratio distortion
EDAT- 2019/03/29 06:00
MHDA- 2019/09/04 06:00
PMCR- 2020/05/01
CRDT- 2019/03/29 06:00
PHST- 2018/07/13 00:00 [received]
PHST- 2019/03/21 00:00 [accepted]
PHST- 2019/03/29 06:00 [pubmed]
PHST- 2019/09/04 06:00 [medline]
PHST- 2019/03/29 06:00 [entrez]
PHST- 2020/05/01 00:00 [pmc-release]
AID - genetics.119.302122 [pii]
AID - 302122 [pii]
AID - 10.1534/genetics.119.302122 [doi]
PST - ppublish
SO  - Genetics. 2019 May;212(1):93-110. doi: 10.1534/genetics.119.302122. Epub 2019 Mar 
      27.