PMID- 30918133
OWN - NLM
STAT- MEDLINE
DCOM- 20191211
LR  - 20191217
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 42
IP  - 6
DP  - 2019 Jun 1
TI  - Ultravist Induces the Expression of MCP-1 and VCAM-1 in IL-4-Stimulated HUVECs.
PG  - 915-922
LID - 10.1248/bpb.b18-00883 [doi]
AB  - The goal of the present study focused on the adverse reaction of contrast medium 
      (CM) via the induction of inflammatory molecules in human umbilical vein 
      endothelial cells (HUVECs). Ultravist-induced monocyte chemoattractant protein-1 
      (MCP-1) and vascular cell adhesion molecule-1 (VCAM-1) gene expression was 
      markedly increased in interleukin-4 (IL-4)-pretreated HUVECs in a time- and 
      dose-dependent manner and was paralleled by concomitant production of MCP-1 and 
      VCAM-1 proteins. MCP-1 and VCAM-1 gene expression by Ultravist in combination 
      with IL-4 was mediated by the c-Jun N-terminal kinases (JNK1/2) signaling 
      pathway. IL-4-pretreated Ultravist-stimulated HUVECs showed greatly increased 
      migration and adhesion of THP-1 cells. Cell migration was decreased by treatment 
      of CCR2 antagonist, and cell adhesion was also decreased by VCAM-1 blocking 
      antibody. Furthermore, when tested in vivo under similar conditions, MCP-1 
      protein was significantly increased in Ultravist combined with IL-4-injected 
      mice. Taken together, our findings suggest that MCP-1 blocking may be crucial in 
      preventing the endothelial dysfunction induced by contrast medium in patients 
      with inflammatory disease and atherosclerosis.
FAU - Kim, Hye-Lim
AU  - Kim HL
AD  - Advanced Research Center for Multiple Myeloma, College of Medicine, Inje 
      University.
FAU - Kim, Nam-Soo
AU  - Kim NS
AD  - Department of Oral and Maxillofacial Surgery, Busan Paik Hospital, Inje 
      University.
FAU - Cho, Hae-Yun
AU  - Cho HY
AD  - Advanced Research Center for Multiple Myeloma, College of Medicine, Inje 
      University.
FAU - Park, Sang-Jun
AU  - Park SJ
AD  - Department of Oral and Maxillofacial Surgery, Busan Paik Hospital, Inje 
      University.
FAU - Lee, Chae Kwan
AU  - Lee CK
AD  - Department of Convergence Biomedical Science, College of Medicine, Inje 
      University.
AD  - Department of Occupational and Environmental Medicine, College of Medical & 
      Institute of Environmental and Occupational Medicine, Inje University.
FAU - Choi, Inhak
AU  - Choi I
AD  - Advanced Research Center for Multiple Myeloma, College of Medicine, Inje 
      University.
AD  - Department of Microbiology and Immunology, Inje University College of Medicine.
FAU - Lee, Soo-Woong
AU  - Lee SW
AD  - Advanced Research Center for Multiple Myeloma, College of Medicine, Inje 
      University.
AD  - Department of Convergence Biomedical Science, College of Medicine, Inje 
      University.
LA  - eng
PT  - Journal Article
DEP - 20190328
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Contrast Media)
RN  - 0 (STAT1 Transcription Factor)
RN  - 0 (STAT1 protein, human)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 207137-56-2 (Interleukin-4)
RN  - 4419T9MX03 (Iohexol)
RN  - 712BAC33MZ (iopromide)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Animals
MH  - Cell Adhesion/drug effects
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/metabolism
MH  - Contrast Media
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Female
MH  - Human Umbilical Vein Endothelial Cells/*drug effects/metabolism
MH  - Humans
MH  - Interleukin-4/*pharmacology
MH  - Iohexol/*analogs & derivatives/pharmacology
MH  - MAP Kinase Signaling System/drug effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - STAT1 Transcription Factor/metabolism
MH  - Signal Transduction
MH  - THP-1 Cells
MH  - Vascular Cell Adhesion Molecule-1/*biosynthesis/metabolism
OTO - NOTNLM
OT  - cell migration
OT  - human umbilical vein endothelial cell (HUVEC)
OT  - interleukin-4 (IL-4)
OT  - monocyte chemoattractant protein-1 (MCP-1)
OT  - ultravist
EDAT- 2019/03/29 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/03/29 06:00
PHST- 2019/03/29 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/03/29 06:00 [entrez]
AID - 10.1248/bpb.b18-00883 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2019 Jun 1;42(6):915-922. doi: 10.1248/bpb.b18-00883. Epub 2019 
      Mar 28.