PMID- 30918216
OWN - NLM
STAT- MEDLINE
DCOM- 20190813
LR  - 20190813
IS  - 1880-4926 (Electronic)
IS  - 1343-4934 (Linking)
VI  - 61
IP  - 1
DP  - 2019
TI  - Antimicrobial efficacy of the supernatant of Streptococcus dentisani against 
      microorganisms implicated in root canal infections.
PG  - 184-194
LID - 10.2334/josnusd.18-0239 [doi]
AB  - The present study aimed to test the antimicrobial activity of Streptococcus 
      dentisani (S. dentisani) supernatant against a collection of microorganisms 
      implicated in dental root infections, and to analyze morphological changes 
      induced in a selection of the tested microorganisms. A total of 22 microbial 
      species were selected, and their growth was monitored by spectrophotometry in the 
      presence and absence of the supernatant of S. dentisani at different assay 
      concentrations (0.2x, 1x, 2x). The generation time and maximum growth rates were 
      evaluated under every tested condition. Scanning electron microscope (SEM) images 
      were obtained to assess the effect on the cell surface following incubation of 
      the pathogens with the concentrated (2x) supernatant of S. dentisani. The 
      supernatant of S. dentisani was found to exert effective inhibitory activity 
      against most of the studied microorganisms implicated in dental root infections 
      (20 out of 22). Total growth inhibition was observed in the case of Streptococcus 
      oralis, Streptococcus sobrinus, Streptococcus salivarius, Prevotella intermedia, 
      and Streptococcus mutans, while the rest of the microorganisms showed an increase 
      in the generation time (between 30 min and 4 h). SEM images revealed structural 
      changes in the membrane consistent with bacteriocin activity, although the 
      effects were heterogeneous among the different species tested.
FAU - Llena, Carmen
AU  - Llena C
AD  - Department of Stomatology, University of Valencia.
FAU - Almarche, Alexandra
AU  - Almarche A
AD  - Department of Stomatology, University of Valencia.
FAU - Mira, Alejandro
AU  - Mira A
AD  - The Foundation for the Promotion of Health and Biomedical Research (FISABIO).
FAU - Lopez, M Arantxa
AU  - Lopez MA
AD  - The Foundation for the Promotion of Health and Biomedical Research (FISABIO).
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Oral Sci
JT  - Journal of oral science
JID - 9808942
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Culture Media)
MH  - Anti-Bacterial Agents/*pharmacology
MH  - *Culture Media
MH  - Dental Pulp Cavity/*microbiology
MH  - Humans
MH  - Infection Control, Dental/*methods
MH  - Microbial Sensitivity Tests
MH  - Microscopy, Electron, Scanning
MH  - Species Specificity
MH  - Streptococcus/*chemistry/*drug effects/growth & development/ultrastructure
OTO - NOTNLM
OT  - S. dentisani
OT  - microbiology
OT  - root infection
EDAT- 2019/03/29 06:00
MHDA- 2019/08/14 06:00
CRDT- 2019/03/29 06:00
PHST- 2019/03/29 06:00 [entrez]
PHST- 2019/03/29 06:00 [pubmed]
PHST- 2019/08/14 06:00 [medline]
AID - 10.2334/josnusd.18-0239 [doi]
PST - ppublish
SO  - J Oral Sci. 2019;61(1):184-194. doi: 10.2334/josnusd.18-0239.