PMID- 30919241
OWN - NLM
STAT- MEDLINE
DCOM- 20211108
LR  - 20211108
IS  - 1993-0402 (Electronic)
IS  - 1672-0415 (Linking)
VI  - 27
IP  - 1
DP  - 2021 Jan
TI  - Humulus lupulus L. Extract Prevents Ovariectomy-Induced Osteoporosis in Mice and 
      Regulates Activities of Osteoblasts and Osteoclasts.
PG  - 31-38
LID - 10.1007/s11655-019-2700-z [doi]
AB  - OBJECTIVE: To systematically evaluate the protective effects of Humulus lupulus 
      L. extract (HLE) on osteoporosis mice. METHODS: In vivo experiment, a total of 35 
      12-week-old female ICR mice were equally divided into 5 groups: the sham control 
      group (sham); the ovariectomy with vehicle group (OVX); the OVX with estradiol 
      valerate [EV, 0.2 mg/(kg*d)] the OVX with low- or high-dose HLE groups [HLE, 1 
      g/(kg*d) and 3 g/(kg*d)], 7 in each group. Treatment began 1 week after the 
      ovariectomized surgery and lasted for 12 weeks. Bone mass and trabecular bone 
      mircoarchitecture were evaluated by micro computed tomography, and bone turnover 
      markers in serum were evaluated using enzyme-linked immunosorbent assay (ELISA) 
      kits. In vitro experiment, osteoblasts and osteoclasts were treated with HLE at 
      doses of 0, 4, 20 and 100 microg/mL. Biomarkers for bone formation in osteoblasts and 
      bone resorption in osteoclasts were analyzed. RESULTS: Compared with the OVX 
      group, HLE exerted bone protective effects by the increase of estradiol (P<0.05), 
      the improvement of cancellous bone structure, bone mineral density (P<0.01) and 
      the reduction of serum alkaline phosphatase (ALP), tartrate resistant acid 
      phosphatase (TRAP), bone gla-protein, c-terminal telopeptides of type I collagen 
      (CTX-I) and deoxypyridinoline levels (P<0.01 for all). In vitro experiment, 
      compared with the control group, HLE at 20 microg/mL promoted the cell proliferation 
      (P<0.01), and increased the expression of bone morphogenetic protein-2 and 
      osteopontin levels in osteoblasts (both P<0.05). HLE at 100 microg/mL increased the 
      osteoblastic ALP activities, and HLE at all dose enhanced the extracellular 
      matrix mineralization (both P<0.01). Furthermore, compared with the control 
      group, HLE at 20 microg/mL and 100 microg/mL inhibited osteoclastic TRAP activity 
      (P<0.01), and reduced the expression of matrix metalloproteinase-9 and cathepsin 
      K (both P<0.05). CONCLUSION: HLE may protect against bone loss, and have 
      potentials in the treatment of osteoporosis.
FAU - Xia, Tian-Shuang
AU  - Xia TS
AD  - Department of Pharmacognosy, School of Pharmacy, Second Military Medical 
      University, Shanghai, (200433), China.
FAU - Lin, Liu-Yue
AU  - Lin LY
AD  - School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, 
      (350108), China.
FAU - Zhang, Qiao-Yan
AU  - Zhang QY
AD  - Department of Pharmacognosy, School of Pharmacy, Second Military Medical 
      University, Shanghai, (200433), China.
FAU - Jiang, Yi-Ping
AU  - Jiang YP
AD  - Department of Pharmacognosy, School of Pharmacy, Second Military Medical 
      University, Shanghai, (200433), China.
FAU - Li, Chang-Hui
AU  - Li CH
AD  - Department of Pharmacognosy, School of Pharmacy, Second Military Medical 
      University, Shanghai, (200433), China.
FAU - Liu, Xiao-Yan
AU  - Liu XY
AD  - Department of Pharmacognosy, School of Pharmacy, Second Military Medical 
      University, Shanghai, (200433), China.
FAU - Qin, Lu-Ping
AU  - Qin LP
AD  - School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, (310053), 
      China.
FAU - Xin, Hai-Liang
AU  - Xin HL
AD  - Department of Pharmacognosy, School of Pharmacy, Second Military Medical 
      University, Shanghai, (200433), China. hailiangxin@163.com.
LA  - eng
PT  - Journal Article
DEP - 20190327
PL  - China
TA  - Chin J Integr Med
JT  - Chinese journal of integrative medicine
JID - 101181180
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Animals
MH  - *Humulus
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Osteoblasts
MH  - Osteoclasts
MH  - *Osteoporosis/drug therapy/prevention & control
MH  - Ovariectomy
MH  - Plant Extracts/pharmacology/therapeutic use
MH  - X-Ray Microtomography
OTO - NOTNLM
OT  - Humulus lupulus L.
OT  - osteoblasts
OT  - osteoclasts
OT  - osteoporosis
OT  - ovariectomy-induced mice
EDAT- 2019/03/29 06:00
MHDA- 2021/11/09 06:00
CRDT- 2019/03/29 06:00
PHST- 2018/08/31 00:00 [accepted]
PHST- 2019/03/29 06:00 [pubmed]
PHST- 2021/11/09 06:00 [medline]
PHST- 2019/03/29 06:00 [entrez]
AID - 10.1007/s11655-019-2700-z [pii]
AID - 10.1007/s11655-019-2700-z [doi]
PST - ppublish
SO  - Chin J Integr Med. 2021 Jan;27(1):31-38. doi: 10.1007/s11655-019-2700-z. Epub 
      2019 Mar 27.