PMID- 30922601
OWN - NLM
STAT- MEDLINE
DCOM- 20190815
LR  - 20190815
IS  - 1877-9603 (Electronic)
IS  - 1877-959X (Linking)
VI  - 10
IP  - 4
DP  - 2019 Jun
TI  - Tissue localization of Coxiella-like endosymbionts in three European tick species 
      through fluorescence in situ hybridization.
PG  - 798-804
LID - S1877-959X(18)30402-3 [pii]
LID - 10.1016/j.ttbdis.2019.03.014 [doi]
AB  - Ticks are commonly infected by Coxiella-like endosymbionts (Coxiella-LE) which 
      are thought to supply missing B vitamin nutrients required for blood 
      digestion.While this nutritional symbiosis is essential for the survival and 
      reproduction of infected tick species, our knowledge of where Coxiella-LE is 
      localized in tick tissues is partial at best since previous studies have focused 
      on a limited number of Asian or American tick species. To fill this gap, we 
      investigated the tissue localization of Coxiella-LE in three European tick 
      species, Ornithodoros maritimus, Dermacentor marginatus and Ixodes hexagonus, 
      using a diagnostic fluorescence in situ hybridization (FISH) assay, combined with 
      PCR-based detection. Specific fluorescent foci were observed in several tick 
      tissues. We visualized a pronounced tissue tropism of Coxiella-LE for tick 
      ovaries and Malpighian tubules, a pattern suggestive of a high degree of 
      lifestyle specialization toward mutualism: infection of the ovaries is indicative 
      of transovarial transmission, whereas infection of the Malpighian tubules 
      suggests a nutritional function. We postulate that Malpighian tubules are key 
      organs for the nutritional symbiosis, notably the synthesis of B vitamins by 
      Coxiella-LE, whereas the infection of the ovaries ensures vertical transmission 
      of the symbionts to future generations. We also detected occasional infections in 
      other organs, such as salivary glands and the midgut. Finally, we discuss the 
      potential significance of the different tissue tropism for tick biology.
CI  - Copyright (c) 2019 Elsevier GmbH. All rights reserved.
FAU - Buysse, Marie
AU  - Buysse M
AD  - MIVEGEC (Maladies Infectieuses et Vecteurs : Ecologie, Genetique, Evolution et 
      Controle), Centre National de la Recherche Scientifique (CNRS) - Institut pour la 
      Recherche et le Developpement (IRD) Universite de Montpellier (UM), Montpellier, 
      France.
FAU - Plantard, Olivier
AU  - Plantard O
AD  - BIOEPAR, INRA, Oniris, 44307, Nantes, France.
FAU - McCoy, Karen D
AU  - McCoy KD
AD  - MIVEGEC (Maladies Infectieuses et Vecteurs : Ecologie, Genetique, Evolution et 
      Controle), Centre National de la Recherche Scientifique (CNRS) - Institut pour la 
      Recherche et le Developpement (IRD) Universite de Montpellier (UM), Montpellier, 
      France.
FAU - Duron, Olivier
AU  - Duron O
AD  - MIVEGEC (Maladies Infectieuses et Vecteurs : Ecologie, Genetique, Evolution et 
      Controle), Centre National de la Recherche Scientifique (CNRS) - Institut pour la 
      Recherche et le Developpement (IRD) Universite de Montpellier (UM), Montpellier, 
      France. Electronic address: olivier.duron@ird.fr.
FAU - Menard, Claudine
AU  - Menard C
AD  - Institut des Biomolecules Max Mousseron (IBMM) - Universite de Montpellier (UM), 
      Montpellier, France. Electronic address: claudine.menard@crbm.cnrs.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190319
PL  - Netherlands
TA  - Ticks Tick Borne Dis
JT  - Ticks and tick-borne diseases
JID - 101522599
RN  - 0 (DNA, Bacterial)
MH  - Animals
MH  - Coxiella/*isolation & purification/physiology
MH  - DNA, Bacterial
MH  - Dermacentor/anatomy & histology/microbiology
MH  - Europe
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Ixodidae/anatomy & histology/microbiology
MH  - Malpighian Tubules/microbiology
MH  - Ovary/microbiology
MH  - Phylogeny
MH  - Salivary Glands/microbiology
MH  - *Symbiosis
MH  - Ticks/anatomy & histology/*microbiology
OTO - NOTNLM
OT  - Coxiella
OT  - Malpighian tubules
OT  - Symbiosis
OT  - Tick
OT  - Tissue tropism
EDAT- 2019/03/30 06:00
MHDA- 2019/08/16 06:00
CRDT- 2019/03/30 06:00
PHST- 2018/09/24 00:00 [received]
PHST- 2019/02/04 00:00 [revised]
PHST- 2019/03/18 00:00 [accepted]
PHST- 2019/03/30 06:00 [pubmed]
PHST- 2019/08/16 06:00 [medline]
PHST- 2019/03/30 06:00 [entrez]
AID - S1877-959X(18)30402-3 [pii]
AID - 10.1016/j.ttbdis.2019.03.014 [doi]
PST - ppublish
SO  - Ticks Tick Borne Dis. 2019 Jun;10(4):798-804. doi: 10.1016/j.ttbdis.2019.03.014. 
      Epub 2019 Mar 19.