PMID- 30925118
OWN - NLM
STAT- MEDLINE
DCOM- 20191202
LR  - 20191202
IS  - 1557-8674 (Electronic)
IS  - 1096-2964 (Linking)
VI  - 20
IP  - 5
DP  - 2019 Jul
TI  - Shenfu Injection Attenuates Bile Duct Injury in Rats with Acute Obstructive 
      Cholangitis.
PG  - 424-430
LID - 10.1089/sur.2018.304 [doi]
AB  - Background: We investigated the effect of Shenfu injection (SFI) in Wistar rats 
      with acute obstructive cholangitis (AOC) and considered the possible molecular 
      mechanisms of the effects. Methods: The 96 rats were divided randomly into three 
      groups. In one group, the common bile duct was subjected to ligation (BDL), and 
      0.2 mL of saline was injected into the proximal bile ducts. To create AOC, again, 
      the common bile duct was ligated, and 0.2 mL of lipopolysaccharide (LPS)) 
      (2 mg/mL) was injected into the proximal ducts. In the Shenfu injection (SFI) 
      group, the material (10 mg/kg) was injected into the tail vein 2 hours before 
      induction of AOC. The hepatic histopathologic changes were observed under a light 
      microscope. The endotoxin, tumor necrosis factor-alpha (TNF-alpha), alanine transaminase 
      (ALT), and total bilirubin (TB) concentrations in the serum were measured at 
      different time points (0, 4, 8, and 16 hours) after ligation. The expression of 
      nuclear transcription factor-kappaB (NF-kappaB) and CD14 in Kupffer cells also was 
      analyzed at different times by Western blotting. Results: The TNF-alpha, ALT, and TB 
      concentrations in the serum and the expression of CD14 and NF-kappaB in Kupffer cells 
      were significantly higher in the SFI group than in the BDL group, but all were 
      significantly lower than in the AOC group. Compared with the AOC group, the edema 
      of cholangiocytes was alleviated in the SFI group, and the infiltration of 
      inflammatory cells around cholangiocytes was reduced. Conclusion: Shenfu 
      injection significantly alleviated bile duct injury. The potential mechanism may 
      be associated with inhibition of CD14 expression and prevention of NF-kappaB 
      activation in Kupffer cells.
FAU - Tan, Hao-Yang
AU  - Tan HY
AD  - Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing 
      Medical University, Chongqing, People's Republic of China.
FAU - Li, Pei-Zhi
AU  - Li PZ
AD  - Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing 
      Medical University, Chongqing, People's Republic of China.
FAU - Gong, Jian-Ping
AU  - Gong JP
AD  - Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing 
      Medical University, Chongqing, People's Republic of China.
FAU - Yang, Kang
AU  - Yang K
AD  - Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing 
      Medical University, Chongqing, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20190329
PL  - United States
TA  - Surg Infect (Larchmt)
JT  - Surgical infections
JID - 9815642
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Immunologic Factors)
RN  - 0 (Shen-Fu)
RN  - EC 2.6.1.2 (Alanine Transaminase)
SB  - IM
MH  - Alanine Transaminase/blood
MH  - Animals
MH  - Cholangitis/*complications
MH  - Cholestasis/*drug therapy
MH  - Disease Models, Animal
MH  - Drugs, Chinese Herbal/*administration & dosage
MH  - Female
MH  - Immunologic Factors/*administration & dosage
MH  - Injections
MH  - Liver/*pathology
MH  - Male
MH  - Rats, Wistar
MH  - Treatment Outcome
OTO - NOTNLM
OT  - CD14
OT  - NF-kappaB
OT  - Shenfu
OT  - acute obstructive cholangitis
OT  - endotoxin-induced bile duct injury
EDAT- 2019/03/30 06:00
MHDA- 2019/12/04 06:00
CRDT- 2019/03/30 06:00
PHST- 2019/03/30 06:00 [pubmed]
PHST- 2019/12/04 06:00 [medline]
PHST- 2019/03/30 06:00 [entrez]
AID - 10.1089/sur.2018.304 [doi]
PST - ppublish
SO  - Surg Infect (Larchmt). 2019 Jul;20(5):424-430. doi: 10.1089/sur.2018.304. Epub 
      2019 Mar 29.