PMID- 30925360
OWN - NLM
STAT- MEDLINE
DCOM- 20190830
LR  - 20190830
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 131
DP  - 2019 Jun
TI  - Dietary supplementation with linseed oil improves semen quality, reproductive 
      hormone, gene and protein expression related to testosterone synthesis in aging 
      layer breeder roosters.
PG  - 9-15
LID - S0093-691X(19)30062-7 [pii]
LID - 10.1016/j.theriogenology.2019.03.016 [doi]
AB  - Omega-3/n-3 polyunsaturated fatty acids (omega-3/n-3 PUFAs) play an important role in 
      male reproductive function. The present study was designed to evaluate the 
      effects of linseed oil (LO) as a source of alpha-linolenic acid (ALA, n-3 PUFA) on 
      semen quality, plasma reproductive hormone and expression of key enzyme and 
      protein related to steroidogenesis in aging layer breeder roosters. Ninety-six 
      57-wk-old Nongda No.3 layer breeder roosters were randomly assigned into one of 
      four dietary treatments. All birds were fed a basal diet for 1wk and then 
      assigned to a corn-soybean meal-based diet containing 0, 1, 2, 4% LO for 4 wk. 
      After feeding trial, the roosters were slaughtered and investigated. The results 
      showed that semen volume was dramatically increased relative to the other 
      treatments in 2% LO group (P < 0.05). With an increase in dietary LO, semen 
      concentration, sperm viability, sperm motility and total sperm count increased 
      linearly (P < 0.05). Plasma follicle-stimulating hormone (FSH) level increased 
      gradually and reached a maximum when 4% LO was fed (P < 0.01). Plasma luteinizing 
      hormone (LH) levels in 1% and 2% LO group were improved significantly (P < 0.05) 
      relative to the control group. Plasma testosterone (T) levels were remarkably 
      improved compared with the control when birds were fed 2% and 4% LO (P < 0.05). A 
      significant increase of steroidogenic acute regulatory protein (StAR) mRNA 
      expression in 2% and 4% LO group was observed relative to the control group 
      (P < 0.05). An increase in dietary LO supplementation from 1% to 4% markedly 
      enhanced (P < 0.05) the mRNA expression of cholesterol side-chain cleavage enzyme 
      (P450scc) compared to the control. A significant increase (P < 0.05) in the 
      Steroidogenic Factor 1 (SF-1) mRNA levels was observed in the 2% and 4% LO-added 
      groups. SF-1 protein expression was markedly increased by adding LO in diets 
      (P < 0.05), and reached a maximum in 2% LO group. In conclusion, the results 
      above suggest that dietary LO may improve semen quality by increasing the T 
      hormone secretion, which may be related to higher StAR and P450scc mRNA 
      expression and SF-1 expression. These findings provide a potential for using LO 
      to attenuate the age-related sub-fertility in commercial layer breeder roosters.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Qi, Xiaolong
AU  - Qi X
AD  - Animal Science and Technology College, Beijing University of Agriculture, 
      Beijing, 102206, China.
FAU - Shang, Mingyu
AU  - Shang M
AD  - Animal Science and Technology College, Beijing University of Agriculture, 
      Beijing, 102206, China.
FAU - Chen, Chen
AU  - Chen C
AD  - Animal Science and Technology College, Beijing University of Agriculture, 
      Beijing, 102206, China.
FAU - Chen, Yu
AU  - Chen Y
AD  - Beijing General Station of Animal Husbandry, Beijing, 100107, China.
FAU - Hua, Jing
AU  - Hua J
AD  - College of Biological Science and Engineering, Beijing University of Agriculture, 
      Beijing, 102206, China.
FAU - Sheng, Xihui
AU  - Sheng X
AD  - Animal Science and Technology College, Beijing University of Agriculture, 
      Beijing, 102206, China.
FAU - Wang, Xiangguo
AU  - Wang X
AD  - Animal Science and Technology College, Beijing University of Agriculture, 
      Beijing, 102206, China.
FAU - Xing, Kai
AU  - Xing K
AD  - Animal Science and Technology College, Beijing University of Agriculture, 
      Beijing, 102206, China.
FAU - Ni, Hemin
AU  - Ni H
AD  - Animal Science and Technology College, Beijing University of Agriculture, 
      Beijing, 102206, China.
FAU - Guo, Yong
AU  - Guo Y
AD  - Animal Science and Technology College, Beijing University of Agriculture, 
      Beijing, 102206, China. Electronic address: y63guo@126.com.
LA  - eng
PT  - Journal Article
DEP - 20190321
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 3XMK78S47O (Testosterone)
RN  - 8001-26-1 (Linseed Oil)
SB  - IM
MH  - Animal Feed
MH  - Animals
MH  - Chickens/*physiology
MH  - *Dietary Supplements
MH  - Linseed Oil/*therapeutic use
MH  - Male
MH  - Random Allocation
MH  - Reproduction/*drug effects
MH  - Semen/*drug effects
MH  - Semen Analysis/*veterinary
MH  - Testosterone/blood
OTO - NOTNLM
OT  - Linseed oil
OT  - Reproductive hormone
OT  - Rooster
OT  - Semen quality
OT  - Testosterone synthesis
EDAT- 2019/03/30 06:00
MHDA- 2019/08/31 06:00
CRDT- 2019/03/30 06:00
PHST- 2018/04/13 00:00 [received]
PHST- 2019/01/04 00:00 [revised]
PHST- 2019/03/20 00:00 [accepted]
PHST- 2019/03/30 06:00 [pubmed]
PHST- 2019/08/31 06:00 [medline]
PHST- 2019/03/30 06:00 [entrez]
AID - S0093-691X(19)30062-7 [pii]
AID - 10.1016/j.theriogenology.2019.03.016 [doi]
PST - ppublish
SO  - Theriogenology. 2019 Jun;131:9-15. doi: 10.1016/j.theriogenology.2019.03.016. 
      Epub 2019 Mar 21.