PMID- 30928369
OWN - NLM
STAT- MEDLINE
DCOM- 20191104
LR  - 20191104
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 132
DP  - 2019 Jul 1
TI  - Tough, hybrid chondroitin sulfate nanofibers as a promising scaffold for skin 
      tissue engineering.
PG  - 63-75
LID - S0141-8130(19)31030-X [pii]
LID - 10.1016/j.ijbiomac.2019.03.208 [doi]
AB  - Fabrication of gelatin/polyvinyl alcohol/chondroitin sulfate (GEL/PVA/CS) hybrid 
      nanofibrous scaffolds using acetic acid and water as an environmentally friendly 
      solvent system via electrospinning for skin tissue engineering was investigated. 
      Modeling and optimization of the nanofibers were performed using response surface 
      methodology (RSM). The influence of CS ratio on mechanical, physical and 
      biological properties of the nanofibers was studied. PVA was used as a carrier 
      and enhancer of mechanical properties. The mechanical properties of hybrid 
      nanofibers were investigated in dry and wet states. The results showed that in 
      the cross-linked dry state the tensile strength was up to 4 MPa. In the wet 
      state, nanofibers exhibited 200% elongation at break, indicating a toughness 
      behavior which enhances the flexibility for clinical applications. Scanning 
      electron microscope (SEM) confirmed the stability of nanofibrous morphology 
      during degradation up to 21 days. Human dermal fibroblast-green fluorescent 
      protein-positive (HDF-GFP+) cells were cultured on the scaffolds and results 
      showed the appropriate biocompatibility. 
      3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium 
      (MTS) assay was employed to study cell proliferation, and the results confirmed 
      the positive effect of CS ratio on HDF cells attachment as well as proliferation 
      on the nanofibers. Considering the results of in vitro assay, nanofibers 
      containing 15% CS ratio suggested as an optimum CS ratio.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Sadeghi, Amin
AU  - Sadeghi A
AD  - Department of Biomaterials, Iran Polymer and Petrochemical Institute, Tehran, 
      Iran; Soft Tissue Engineering Research Center, Tissue Engineering and 
      Regenerative Medicine Institute, Central Tehran Branch, Islamic Azad University, 
      Tehran, Iran.
FAU - Zandi, Mojgan
AU  - Zandi M
AD  - Department of Biomaterials, Iran Polymer and Petrochemical Institute, Tehran, 
      Iran.
FAU - Pezeshki-Modaress, Mohamad
AU  - Pezeshki-Modaress M
AD  - Burn Research Center, Iran University of Medical Sciences, Tehran, Iran. 
      Electronic address: Pezeshkimodaress.m@iums.ac.ir.
FAU - Rajabi, Sarah
AU  - Rajabi S
AD  - Department of Stem Cells and Developmental Biology, Cell Science Research Center, 
      Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20190327
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Biocompatible Materials)
RN  - 9007-28-7 (Chondroitin Sulfates)
SB  - IM
MH  - Biocompatible Materials/chemistry/pharmacology
MH  - Cell Adhesion/drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Chondroitin Sulfates/*chemistry
MH  - Humans
MH  - *Mechanical Phenomena
MH  - Nanofibers/*chemistry
MH  - Porosity
MH  - Skin/*cytology/drug effects
MH  - Surface Properties
MH  - *Tissue Engineering
MH  - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT  - Electrospinning
OT  - Gelatin/polyvinyl alcohol/chondroitin sulfate
OT  - Human dermal fibroblast
OT  - Optimization
OT  - Tissue engineering
EDAT- 2019/04/01 06:00
MHDA- 2019/11/05 06:00
CRDT- 2019/04/01 06:00
PHST- 2019/02/10 00:00 [received]
PHST- 2019/03/19 00:00 [revised]
PHST- 2019/03/26 00:00 [accepted]
PHST- 2019/04/01 06:00 [pubmed]
PHST- 2019/11/05 06:00 [medline]
PHST- 2019/04/01 06:00 [entrez]
AID - S0141-8130(19)31030-X [pii]
AID - 10.1016/j.ijbiomac.2019.03.208 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2019 Jul 1;132:63-75. doi: 10.1016/j.ijbiomac.2019.03.208. 
      Epub 2019 Mar 27.