PMID- 30928462
OWN - NLM
STAT- MEDLINE
DCOM- 20200608
LR  - 20200608
IS  - 1872-7549 (Electronic)
IS  - 0166-4328 (Linking)
VI  - 367
DP  - 2019 Jul 23
TI  - Estrogen receptors alpha and beta in the central amygdala and the ventromedial nucleus 
      of the hypothalamus: Sociosexual behaviors, fear and arousal in female rats 
      during emotionally challenging events.
PG  - 128-142
LID - S0166-4328(19)30235-9 [pii]
LID - 10.1016/j.bbr.2019.03.045 [doi]
AB  - Estrogens receptors (ER) are involved in several sociosexual behaviors and fear 
      responses. In particular, the ERalpha is important for sexual behaviors, whereas ERbeta 
      modulates anxiolytic responses. Using shRNA directed either against the ERalpha or 
      the ERbeta RNAs (or containing luciferase control) encoded within an 
      adeno-associated viral vector, we silenced these receptors in the ventromedial 
      nucleus of the hypothalamus (VMN) and the central amygdala (CeA). We exposed 
      ovariectomized female rats, sequentially treated with estradiol benzoate and 
      progesterone, to five stimuli, previously reported to elicit positive and 
      negative affect. The subjects were housed in groups of 4 females and 3 males in a 
      seminatural environment for several days before hormone treatment. We analyzed 
      the frequency of a large number of behavior patterns. In addition, we performed 
      analyses of co-occurrence in order to detect changes in the structure of behavior 
      after infusion of the vectors. Silencing the ERalpha in the VMN disrupted lordosis 
      and showed some anxiolytic properties in aversive situations, whereas silencing 
      of the ERbeta in this structure had no effect. This was also the case after 
      silencing the ERalpha in the CeA. Silencing of the ERbeta in this structure increased 
      risk assessment, an expression of anxiety, and increased olfactory exploration of 
      the environment. We hypothesize that the ERbeta in the CeA has an important role in 
      the well-established anxiolytic effects of estrogens, and that it may modulate 
      arousal level. Furthermore, it seems that the ERalpha in the VMN is anxiogenic in 
      aversive or threatening situations, in agreement with other studies.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Le Moene, Olivia
AU  - Le Moene O
AD  - Department of Psychology, University of Tromso, Huginbakken 32, 9037, Tromso, 
      Norway. Electronic address: olivia.s.moene@uit.no.
FAU - Stavarache, Mihaela
AU  - Stavarache M
AD  - Laboratory of Molecular Neurosurgery, Department of Neurological Surgery, Weill 
      Cornell Medical College, 1300 York Avenue, NY, 10065, New York, United States.
FAU - Ogawa, Sonoko
AU  - Ogawa S
AD  - Laboratory of Behavioral Neuroendocrinology, University of Tsukuba, 1-1-1 
      Tennodai, Tsukuba, 305-8577, Japan.
FAU - Musatov, Sergei
AU  - Musatov S
AD  - Laboratory of Molecular Neurosurgery, Department of Neurological Surgery, Weill 
      Cornell Medical College, 1300 York Avenue, NY, 10065, New York, United States; 
      Neurologix Inc., Fort Lee, NJ, 07024, United States; Laboratory of Neurobiology 
      and Behavior, The Rockefeller University, New York, NY, 10021, United States.
FAU - Agmo, Anders
AU  - Agmo A
AD  - Department of Psychology, University of Tromso, Huginbakken 32, 9037, Tromso, 
      Norway.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190327
PL  - Netherlands
TA  - Behav Brain Res
JT  - Behavioural brain research
JID - 8004872
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
SB  - IM
MH  - Animals
MH  - Arousal/*physiology
MH  - Behavior, Animal/*physiology
MH  - Central Amygdaloid Nucleus/metabolism/*physiology
MH  - Estrogen Receptor alpha/*physiology
MH  - Estrogen Receptor beta/*physiology
MH  - Fear/*physiology
MH  - Female
MH  - Male
MH  - Rats
MH  - Sexual Behavior, Animal/physiology
MH  - *Social Behavior
MH  - Ventromedial Hypothalamic Nucleus/metabolism/*physiology
OTO - NOTNLM
OT  - Central amygdala
OT  - Estrogen receptors
OT  - Fear
OT  - Recovery
OT  - Seminatural environment
OT  - Ventromedial nucleus of the hypothalamus
EDAT- 2019/04/01 06:00
MHDA- 2020/06/09 06:00
CRDT- 2019/04/01 06:00
PHST- 2019/02/12 00:00 [received]
PHST- 2019/03/25 00:00 [revised]
PHST- 2019/03/25 00:00 [accepted]
PHST- 2019/04/01 06:00 [pubmed]
PHST- 2020/06/09 06:00 [medline]
PHST- 2019/04/01 06:00 [entrez]
AID - S0166-4328(19)30235-9 [pii]
AID - 10.1016/j.bbr.2019.03.045 [doi]
PST - ppublish
SO  - Behav Brain Res. 2019 Jul 23;367:128-142. doi: 10.1016/j.bbr.2019.03.045. Epub 
      2019 Mar 27.