PMID- 30930419
OWN - NLM
STAT- MEDLINE
DCOM- 20190801
LR  - 20190801
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 42
IP  - 4
DP  - 2019
TI  - Comparison of the Allergenic Potency of House Dust Extract and House Dust Mite 
      Allergen Extract for Subcutaneous Allergen Immunotherapy.
PG  - 601-606
LID - 10.1248/bpb.b18-00710 [doi]
AB  - Subcutaneous allergen immunotherapy (SCIT) with non-standardized house dust (HD) 
      extracts has been used in Japan since 1963 for house dust mite (HDM)-allergic 
      patients. Since the potencies of HD extracts are unknown, the allergenic potency 
      of HD extracts was examined by comparing with a standardized HDM allergen 
      extracts. The major allergen content of HDM in the extracts was measured using a 
      sandwich enzyme-linked immunosorbent assay (ELISA). The immunoglobulin E (IgE) 
      inhibitory activities of the extracts were measured by a competitive ELISA. The 
      extract concentrations giving 50% inhibition of IgE binding (log(10) IC(50)) were 
      determined from dose-response curves and defined as inhibitory activities. A 
      linear regression line was constructed from the log(10) IC(50) values of the 
      standardized HDM extract to interpolate the relative potency of the HD extract 
      with strength of 1 : 10 w/v (HD 1 : 10). The amounts of major allergens (Der f 1, 
      Der p 1 and Der 2) were 116.3 microg/mL in the HDM allergen extract (100000 Japanese 
      Allergy Units [JAU]/mL) and 0.77 microg/mL in the HD 1 : 10. The inhibitory activity 
      (log(10) IC(50) values) of HD 1 : 10 was 2.389 +/- 0.078, indicating the allergenic 
      potency was between 200 and 2000 JAU/mL. Based on regression analysis (R(2) 
      >0.99), the allergenic potency of HD 1 : 10 was estimated to be 842 +/- 128 JAU/mL. 
      The present study determined the major allergen content of HD extract, which 
      contributes to its allergenic potency. The allergenic potency of HD 1 : 10 was 
      ca. 100-fold less than that of HDM allergen extract.
FAU - Du, Weibin
AU  - Du W
AD  - Research Laboratory, Torii Pharmaceutical Co., Ltd.
FAU - Fukano, Chiharu
AU  - Fukano C
AD  - Research Laboratory, Torii Pharmaceutical Co., Ltd.
FAU - Yonemoto, Mari
AU  - Yonemoto M
AD  - Research Laboratory, Torii Pharmaceutical Co., Ltd.
FAU - Matsuoka, Tomokazu
AU  - Matsuoka T
AD  - Department of Otolaryngology, Head & Neck Surgery, Facility of Medicine, 
      University of Yamanashi.
FAU - Masuyama, Keisuke
AU  - Masuyama K
AD  - Department of Otolaryngology, Head & Neck Surgery, Facility of Medicine, 
      University of Yamanashi.
FAU - Ohashi-Doi, Katsuyo
AU  - Ohashi-Doi K
AD  - Research Laboratory, Torii Pharmaceutical Co., Ltd.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Allergens)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Arthropod Proteins)
RN  - 0 (Complex Mixtures)
RN  - 0 (Dermatophagoides pteronyssinus antigen p 2)
RN  - 0 (Dust)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.- (Dermatophagoides farinae antigen f 1)
RN  - EC 3.4.22.- (Dermatophagoides pteronyssinus antigen p 1)
MH  - Allergens/analysis/*immunology
MH  - Animals
MH  - Antigens, Dermatophagoides/analysis/*immunology
MH  - Arthropod Proteins/analysis/*immunology
MH  - Complex Mixtures/analysis/pharmacology
MH  - Cysteine Endopeptidases/analysis/*immunology
MH  - *Desensitization, Immunologic
MH  - *Dust
MH  - Housing
MH  - Immunoglobulin E/immunology
MH  - Injections, Subcutaneous
MH  - Pyroglyphidae/*immunology
OTO - NOTNLM
OT  - allergen immunotherapy
OT  - allergenic potency
OT  - house dust mite
OT  - standardized allergen
EDAT- 2019/04/02 06:00
MHDA- 2019/08/02 06:00
CRDT- 2019/04/02 06:00
PHST- 2019/04/02 06:00 [entrez]
PHST- 2019/04/02 06:00 [pubmed]
PHST- 2019/08/02 06:00 [medline]
AID - 10.1248/bpb.b18-00710 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2019;42(4):601-606. doi: 10.1248/bpb.b18-00710.